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A kind of tissue culture rapid propagation method of Scutellaria baicalensis

A technology of tissue culture and Scutellaria baicalensis, which is applied in the field of rapid propagation of Scutellaria baicalensis tissue culture, can solve the problems of low germination rate of scutellaria baicalensis seeds, destruction of wild resources, shortage of market supply, etc., and achieve the effect of improving reproduction, overcoming low germination rate, and simple method

Active Publication Date: 2019-07-12
WUXI MISHO ECOLOGY LANDSCAPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Scutellaria baicalensis has pharmacological effects such as clearing heat and detoxifying, analgesic and anti-inflammatory. In recent years, studies have shown that scutellaria baicalensis also has strong anti-cancer, anti-oxidation, anti-virus and anti-HIV effects, and has high biological safety. The attention of the world, the market demand is increasing year by year, wild resources are being plundered and destroyed. Although there are cultivations, the market is still in short supply. In addition, the germination rate of Scutellaria baicalensis seeds is low and the reproduction speed is slow. In recent years, Scutellaria baicalensis is gradually used as a perennial flower In terms of landscaping, how to quickly and effectively propagate Scutellaria baicalensis has become one of the important factors restricting its development.

Method used

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  • A kind of tissue culture rapid propagation method of Scutellaria baicalensis
  • A kind of tissue culture rapid propagation method of Scutellaria baicalensis
  • A kind of tissue culture rapid propagation method of Scutellaria baicalensis

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Embodiment 1

[0023] A method for rapid propagation of Scutellaria baicalensis through tissue culture, comprising the steps of:

[0024] (1) Acquisition of sterile explants: completely immerse the robust and fresh stems of Scutellaria baicalensis collected in the field, and rinse them with tap water for 2 hours; after taking them out, place them on the ultra-clean workbench, and put the seedlings in a sterilized beaker. After rinsing with 75% ethanol for 15s, rinsing with sterile water for 4 times, sterilizing with 0.1% mercuric chloride for 4 minutes, and then rinsing with sterile water for 4 times, dry the excess water with sterile filter paper to obtain sterile explants inoculate the body;

[0025] (2) Induction culture: with the treated aseptic explants, get the stem segment, remove the blade, cut the stem segment into 1cm segment, keep a node, each stem segment has axillary buds; the cut The stem segments were inoculated on the induction medium of adventitious buds; the induction medi...

Embodiment 2

[0030] A method for rapid propagation of Scutellaria baicalensis through tissue culture, comprising the steps of:

[0031] (1) Acquisition of sterile explants: completely immerse the stems of the robust and fresh Scutellaria baicalensis collected in the field in water, rinse them with tap water for 3 hours; Put the seedlings in, rinse with 70% ethanol for 30 seconds, rinse with sterile water for 5 times, disinfect with 0.1% mercuric chloride for 6 minutes, rinse with sterile water for 5 times, and dry the excess water with sterile filter paper to obtain sterile The explants were inoculated.

[0032] (2) induction culture: with the aseptic explant that handles, get stem segment, excise blade, stem segment is cut into 2cm segment, retains a node, has axillary bud on each stem segment; The stem segments were inoculated on the induction medium of adventitious buds; the induction medium was: MS+2mg / L BA+0.1mg / L NAA+30g / L sucrose+8g / L agar powder, pH5.8, and the induction condition...

Embodiment 3

[0037] A method for rapid propagation of Scutellaria baicalensis through tissue culture, comprising the steps of:

[0038](1) Acquisition of sterile explants: The robust and fresh stems of Scutellaria baicalensis collected in the field were completely immersed in water, and rinsed with tap water for 2 hours; Put the seedlings in, rinse with 75% ethanol for 45 seconds, rinse with sterile water for 6 times, disinfect with 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 6 times, and dry the excess water with sterile filter paper to obtain sterile The explants were inoculated.

[0039] (2) induction culture: with the aseptic explant that handles, get stem segment, excise blade, stem segment is cut into 2cm segment, retains a node, has axillary bud on each stem segment; The stem segments were inoculated on the induction medium of adventitious buds; the induction medium was: MS+3mg / L BA+0.5mg / L NAA+30g / L sucrose+8g / L agar powder, pH5.8, and the induction conditio...

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Abstract

The invention relates to a radix scutellariae tissue culture fast breeding method, which comprises the following steps of firstly, rinsing the stems of healthy and strong fresh radix scutellariae collected in the wild for many times; then, putting the stems of the radix scutellariae onto an ultra-clean work table to be subjected to irradiation treatment for 10 to 25 min by an ultraviolet lamp to obtain aseptic explants; then, sequentially performing induced culture, multiplication culture and rooting culture to obtain the rootless aseptic seedlings; finally, performing domestication and transplanting on the healthy and strong aseptic seedlings with many leaves and with the root system lengths being greater than 2cm. The method provided by the invention is simple; the operation of the steps is easy; the defects of low sprouting rate and low breeding speed of the radix scutellariae can be overcome. By a tissue culture method, the radix scutellariae tissue culture system is built; the breeding coefficient is effectively improved; the growth is promoted; the breeding intensity of the radix scutellariae is improved.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method of Scutellaria baicalensis, belonging to the technical field of biological propagation. Background technique [0002] Scutellaria baicalensis has pharmacological effects such as clearing heat and detoxifying, analgesic and anti-inflammatory. In recent years, studies have shown that scutellaria baicalensis also has strong anti-cancer, anti-oxidation, anti-virus and anti-HIV effects, and has high biological safety. The attention of the world, the market demand is increasing year by year, wild resources are being plundered and destroyed, although there is cultivation, the market is still in short supply, and the germination rate of Scutellaria baicalensis is low, and the reproduction speed is slow. In recent years, Scutellaria baicalensis is gradually used as a perennial flower. In terms of landscaping, how to quickly and effectively propagate Scutellaria baicalensis has become one of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 田海丽李懿玮王莹徐静王茂
Owner WUXI MISHO ECOLOGY LANDSCAPE