High-solubility cod protein

A technology for cod protein and cod, which is applied in the field of highly soluble cod protein, can solve the problems of difficulty in diversified development of protein products, unfavorable absorption, reduced nutrient utilization rate and application value, etc., and achieves reasonable amino acid ratio and improved solubility. , good foaming effect

Inactive Publication Date: 2017-09-12
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the problem of insoluble fish protein in water has always been an important factor restricting its protein processing and utilization. The hydrophobicity of these proteins not only ...

Method used

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  • High-solubility cod protein
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  • High-solubility cod protein

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Embodiment 1

[0017] A preparation method of highly soluble cod protein, characterized in that the method steps are as follows:

[0018] A) Preparation of cod protein isolate: use cod as raw material, remove the head, peel off the skin, clean the internal organs, wash with ice water, remove the cod meat, add water and homogenize it, adjust the pH to 11.0 with sodium hydroxide solution, and collect the supernatant by centrifugation , adjusting the pH to 5.0 with hydrochloric acid solution, centrifuging, collecting the precipitate, redissolving with water, adjusting the pH to neutral with sodium hydroxide solution, vacuum freeze-drying and freeze-drying to obtain cod protein isolate.

[0019] B) enzymatic modification: cod protein isolate is dissolved in water according to the ratio of 4% (w / v) to make cod protein dispersion, adjust pH to 7.8 with sodium hydroxide solution, then add neutral protease 3100U / g, in Shaking and hydrolyzing in a constant temperature water bath at 50°C for 5 hou...

Embodiment 2

[0040] A preparation method of highly soluble cod protein, characterized in that the method steps are as follows:

[0041] A) Preparation of cod protein isolate: use cod as raw material, remove the head, peel off the skin, clean the internal organs, wash with ice water, remove the cod meat, add water and homogenize it, adjust the pH to 11.0 with sodium hydroxide solution, and collect the supernatant by centrifugation , adjusting the pH to 5.0 with hydrochloric acid solution, centrifuging, collecting the precipitate, redissolving with water, adjusting the pH to neutral with sodium hydroxide solution, vacuum freeze-drying and freeze-drying to obtain cod protein isolate.

[0042] B) enzymatic modification: cod protein isolate is dissolved in water according to the ratio of 4% (w / v) to make cod protein dispersion, adjust pH to 8.0 with sodium hydroxide solution, then add neutral protease 3000U / g, in Shaking and hydrolyzing in a constant temperature water bath at 50°C for 4.5 h...

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Abstract

The invention provides a high-solubility cod protein. The high-solubility cod protein is prepared by steps: taking cod meat as a raw material, extracting cod protein isolate by means of isoelectric precipitation, and performing vacuum freeze-drying to obtain cod protein isolate powder; dissolving the cod protein isolate powder into water to obtain cod protein dispersion liquid, adopting alkaline solution for adjusting pH to 7.0-10.0, adding proteinase, and hydrolyzing to obtain cod protein isolate hydrolysis liquid; subjecting the cod protein isolate hydrolysis liquid to water bath at 85-100 DEG C to inactivate the proteinase for 5-15min, cooling to the room temperature, centrifuging for 5-15min at a rotating speed of 2000-5000r/min, taking supernatant, and performing reduced-pressure evaporation concentration and vacuum freeze drying to obtain the high-solubility cod protein. Solubility of the cod protein obtained through the process is improved from 11.22% to 80.35%, and the high-solubility cod protein is excellent in emulsification and foaming performances.

Description

technical field [0001] The invention belongs to the technical field of food protein modification in the food field, and in particular relates to highly soluble cod protein. Background technique [0002] Cod is an important economic fish in the world, with an annual catch of 10 million tons, accounting for about 12% of the total marine fishery output. Cod is rich in protein, with a content as high as 18.2%. It contains all the amino acids needed by the human body, and the ratio of amino acids is very close to the ratio of human needs. It is recognized as an important source of high-quality dietary protein in the world. However, the problem of insoluble fish protein in water has always been an important factor restricting its protein processing and utilization. The hydrophobicity of these proteins not only brings great difficulties to the diversified development of protein products, but also is not conducive to human body absorption, reducing its Utilization and application v...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/12
CPCC07K1/12C12P21/06
Inventor 刘楚怡李八方王长伟马晓明车帅
Owner OCEAN UNIV OF CHINA
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