Preparation method of a novel biological material based on lactoferrin activity

Abstract

The invention discloses a preparation method of a new biomaterial based on lactoferrin activity. The lactoferrin having osteogenic activity is applied to the bone tissue engineering field as a new bone growth factor, so the problems of difficult obtaining and high prices of various bone growth factor are solved. Lactoferrin is loaded on a hydroxyapatite / collagen implant material, and the interaction between the lactoferrin and hydroxyapatite / collagen composite material is used to realize the slow release of the lactoferrin and solve the problem of short half-life of the supported growth factor. The osteogenic activity of the hydroxyapatite / collagen implant material is improved to a certain degree, and the problem of low bioactivity of a single biomaterial is solved.

Description

technical field [0001] The invention relates to a biological material, in particular to a method for preparing a novel biological material based on lactoferrin activity. Background technique [0002] With the intensification of population aging in modern society, the incidence of bone trauma, bone tumors and other bone diseases remains high, which directly leads to a large number of patients with fractures and bone defects. According to statistics, there are more than 5 million patients with bone defects in my country every year, and the number of patients with fractures of the spine and extremities caused by osteoporosis is more than 2 million. About 400,000 patients underwent bone graft surgery, and most of these patients faced bone graft treatment. [0003] At present, traditional bone grafting treatments mainly include autologous bone grafting and allogeneic bone grafting. Autologous bone transplantation has always been known as the "gold standard" in the industry, but ...

AI Technical Summary

Problems solved by technology

However, these endogenous bone growth factors are mainly obtained through two methods, one is to separate and extract from tissues such as body fluids or blood, but the growth factors in the body belong to trace peptides or proteins, and it is difficult to extract them.

The other is obtained through technical means such as genetic engineering and molecular biology. Not only the production equipment is complicated, but also the preparation process is quite complicated, so the production cost is high, the price is expensive, and it is difficult to produce on a large scale, which limits its application in the field. Wide application in experiments and clinics

Moreover, the half-life of bone growth factor is short, and it is easy to degrade in the body and be diluted by body fluids, and the ideal effect concentration cannot be reached. However, if the first large dose is administered, it is easy to cause local toxicity and hemangioma-like lesions, which also limits its application. scope

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