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Beauveria bassiana solid medium and preparation method thereof

A technology of solid medium and culture method, applied in the field of Beauveria bassiana solid medium and its preparation, can solve the problems of conidia with long storage period, short survival time, short fermentation time, etc., achieve low price and reduce production cost , The effect of simple operation process

Active Publication Date: 2017-09-15
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The liquid fermentation time of Beauveria bassiana is short, and the main products are blastospore and mycelium, but the survival time of these two products is relatively short, and it is not suitable to be processed into a preparation form; the solid fermentation and liquid-solid two-phase fermentation take a long time, but both can produce conidia with long shelf life

Method used

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  • Beauveria bassiana solid medium and preparation method thereof

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Effect test

Embodiment 1

[0029] The solid culture method of embodiment 1 inoculation Beauveria bassiana spore suspension

[0030] 1 Preparation of spore suspension

[0031] 1.1 Strain activation: The Beauveria bassiana strain JCF (preservation number: CGMCC No. 11338) preserved at low temperature was activated and cultivated on the inclined surface of the test tube. It can be used after being overgrown with spores.

[0032] 1.2 Preparation of spore suspension: transfer the conidia on the slant in 1.1 to the SDAY plate medium, rinse the spores with 0.1% Tween-80 sterile water after sporulation, shake and suspend to make a spore suspension, and adjust the concentration to 1×10 7 Spores / mL.

[0033] 2 solid culture

[0034] 2.1 Preparation of solid medium: grind corn into granules with a grinder, the size is about 2-4mm, soak in water for 4 hours, the average water content is 25wt%, remove excess water, add 0.2% fish meal as nitrogen source, add 0.5 % salad oil in order to prevent adhesion, after st...

Embodiment 2

[0038] The solid culture method of embodiment 2 inoculating Beauveria bassiana mycelia

[0039] 1 Mycelium seed liquid preparation:

[0040] 1.1 Strain activation: the method is the same as in Example 1.

[0041] 1.2 Preparation of bacterial suspension: the method is the same as in Example 1, and the concentration of the spore suspension is 5×10 7 Spores / mL.

[0042] 1.3 Shake flask culture: put 100ml of SDY medium in a 500ml Erlenmeyer flask, sterilize with high-temperature steam at 121°C for 30min, after cooling, inoculate the spore suspension with 1% inoculation amount in a sterile operating table, at 26±1°C , 200rpm constant temperature shaking culture, after 20 hours of culture, the mycelium formed can be used as the seed liquid of Beauveria bassiana solid culture.

[0043] 2 solid culture

[0044] 2.1 Preparation of solid medium: the method is the same as in Example 1, and the nitrogen source is 0.8% peanut powder.

[0045] 2.2 Inoculation and cultivation: Pour the un...

Embodiment 3

[0048] Embodiment 3 Inoculate the solid culture method of Beauveria bassiana blastospore

[0049] 1 Preparation of blastospore seed solution:

[0050] 1.1 Strain activation: the method is the same as in Example 1.

[0051] 1.2 Preparation of bacterial suspension: the method is the same as in Example 1, and the concentration of the spore suspension is 5×10 6 Spores / mL.

[0052] 1.3 Shake flask culture: put 100ml of SDY medium in a 500ml Erlenmeyer flask, sterilize with high-temperature steam at 121°C for 30min, after cooling, inoculate the spore suspension with 1% inoculation amount in a sterile operating table, at 26±1°C , 200rpm constant temperature shaking culture, after 28 hours of culture, a large number of blastospores are formed, which can be used as seed liquid for solid-phase fermentation.

[0053] 2 solid culture

[0054] 2.1 Production of solid medium: same as in Example 1, nitrogen source is 0.5% soybean flour.

[0055] 2.2 Inoculation and cultivation: Same as ...

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Abstract

The invention provides a Beauveria bassiana solid medium adopting corn granules as a matrix. The invention also provides a preparation method of the Beauveria bassiana solid medium and a culture method of Beauveria bassiana. The Beauveria bassiana solid medium adopts the corn granules as a matrix, and raw materials can be easily obtained, have low prices and can be locally obtained in most regions, so the transportation cost is reduced, the production cost is greatly reduced, the solid medium is suitable for different enterprises and research units to research and produce the Beauveria bassiana, and the solid medium has obvious economic values and a good application prospect. The solid medium can be used in a Beauveria bassiana solid fermentation and liquid-solid two-phase fermentation technology. The culture method has the advantages of easiness in operation, short fermentation period and high sporulation quantity.

Description

technical field [0001] The invention belongs to the technical field of Beauveria bassiana culture, in particular to a Beauveria bassiana solid culture medium and a preparation method thereof. Background technique [0002] Beauveria bassiana is a kind of entomopathogenic fungi with a wide range of hosts for agricultural and forestry pests and strong pathogenicity. The most widely used pest biocontrol pathogenic fungi have been successfully used for large-scale control of various important agricultural and forestry pests such as pine caterpillars, corn borers, grubs, and soybean borers. [0003] At present, the main methods of mass cultivation of Beauveria bassiana include liquid fermentation, solid fermentation and liquid-solid two-phase fermentation. The liquid fermentation time of Beauveria bassiana is short, and the main products are blastospore and mycelium, but the survival time of these two products is relatively short, and it is not suitable to be processed into a pre...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 杜立新曹伟平宋健冯书亮
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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