Method for preparing antioxidative peptide by using cod to process by-product

A technology of antioxidant peptides and by-products, which is applied in the field of antioxidant peptide preparation, can solve the problems of preparation of antioxidant peptides that have not been reported, and achieve the effects of abundant raw materials, avoiding pollution, and low cost

Inactive Publication Date: 2017-09-15
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Domestic research on the preparation of antioxidant peptides by hydrolyzing cod fillet with protease has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A) Wash the cod steak by-product of cod processing to remove the residual viscera and fish skin, cook under high temperature and high pressure at 120°C for 30 minutes, homogenize with a homogenizer, add water to the solid matter and water at a ratio of 1:4 to prepare cod steak homogenate;

[0022] B) Adjust the pH of the cod steak homogenate to 8.00, then add protease 3000u / g, hydrolyze in a 40°C water bath for 5 hours, carry out the hydrolysis in a shaking state, and keep the temperature and pH constant to obtain the cod steak protein hydrolyzate;

[0023] C) Inactivate protease in cod fillet protein hydrolyzate in 100°C water bath for 10 minutes, cool to room temperature, centrifuge at 4000r / min for 15 minutes, take supernatant, and perform ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 5000Da , to obtain the cod steak polypeptide ultrafiltrate;

[0024] D) Evaporating the cod fillet protein polypeptide ultrafiltrate under reduced ...

Embodiment 2

[0034] Wash the cod steak by-product of cod processing to remove the residual viscera and fish skin, cook it under high temperature and high pressure for 20 minutes at 100°C, homogenize it with a homogenizer, add water to the solid matter and water at a ratio of 1:4 to prepare cod steak homogenate;

[0035] Adjust the pH of the cod steak homogenate to 9.0, then add protease 4000u / g, hydrolyze in a water bath at 50°C for 3 hours, carry out the hydrolysis under shaking, and keep the temperature and pH constant to obtain the cod steak protein hydrolyzate;

[0036] The cod steak protein hydrolyzate was inactivated in a water bath at 85°C for 15 minutes. After cooling to room temperature, it was centrifuged at 6000r / min for 10 minutes. The supernatant was taken and ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 5000Da to obtain Peptide ultrafiltrate of cod steak;

[0037] The cod steak protein polypeptide ultrafiltrate liquid is subjected to vacuum ...

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Abstract

The invention discloses a method for preparing antioxidative peptide by using cod to process a by-product. A cod processed by-product cod fillet is taken as a raw material, the cod fillet having rich protein is subjected to high- temperature and high pressure boiling and crushing, water is added for preparing a cod fillet solution, the pH value is adjusted, pancreatin is added and is hydrolyzed under constant temperature, enzyme deactivation is carried out after the reaction is completed, refrigerated centrifugation is carried out, a supernatant is taken, and then is subjected to the steps of ultrafiltration, concentration and freeze drying to obtain the cod fillet antioxidative peptide powder. The obtained peptide powder has good removing rate on DPPH free radical, hydroxyl radical and superoxide anion, and is an excellent anti-oxidant. The peptide powder has the characteristics of light yellow, no bitter taste, and slight fishiness, the molecular weight can be controlled to less than or equal to 1000 Da, and the polypeptide total protein is 89.56%. The used cod fillet is the cod processed by-product, the cost is low, the prepared antioxidative peptide has the advantages of good antioxidation effect, high security, and no toxic and side effect, and high-quality antioxidative peptide is provided for food, cosmetic and medicine industries.

Description

technical field [0001] The invention belongs to the technical field of preparation of antioxidant peptides, and in particular relates to a method for preparing antioxidant peptides by using cod processing by-products. Background technique [0002] Antioxidants have developed rapidly at home and abroad in recent years, and their uses are becoming wider and wider. Since Harman put forward the theory of free radicals, people have realized that the free radicals produced by oxidation in the human body are related to human aging and many diseases, so antioxidants are not only used for antioxidation of fatty foods, but also as functional factors for health food and cosmetics and other development. [0003] There are many kinds of antioxidants. However, chemically synthesized antioxidants such as BHA and BHT have toxicity and side effects. Governments of various countries have imposed ADI values ​​on them to control their excessive addition. Antioxidant active peptides, due to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 马晓明刘楚怡李八方车帅王长伟冯晓梅
Owner OCEAN UNIV OF CHINA
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