Application of a remotely regulated gene circuit system in the treatment of diabetes
A remote control and diabetes technology, applied in multidisciplinary fields, can solve the problems of incurable diabetes and low blood sugar risk.
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[0183] (4) Preparation of Escherichia coli Competent Cells
[0184] All solutions and consumables used for the preparation of competent cells are sterilized by high temperature and high pressure.
[0185] 1. Streak Escherichia coli DH5α strain on a plate without antibiotics, and culture it upside down at 37°C for 12-16 hours;
[0186] Pick a single colony and place it in a 2mL LB shaker tube without any antibiotics, shake it at 180rpm at 37°C overnight;
[0187] 2. Take 1mL of the bacterial liquid and transfer it to a 250mL Erlenmeyer flask with LB medium for expansion culture (according to the ratio of 1:100 to expand the culture), shake the culture at 180rpm at 37°C for 2-3 hours until the OD600 is between 0.4-0.5;
[0188] 3. Transfer the culture medium into a centrifuge tube, place it on ice for 10 minutes, then centrifuge at 3000 rpm for 10 minutes at 4°C (the centrifuge needs to be pre-cooled in advance), and carefully discard the supernatant;
[0189] 4. Add pre-coole...
Embodiment 1
[0239] Embodiment 1, the production of an intelligent control system for ultra-remotely controlling a far-red light source by a smart phone through a local area network WiFi or a 2G / 3G / 4G network
[0240] In the present invention, taking the intelligent controller made by the smart home studio and its matching APP, 6 multi-channel output switching power supplies, and 24 far-infrared LED lights as the far-red light source as an example, it is illustrated that the smart phone passes through the local area network WiFi or 2G / 3G / 4G network ultra-remote control of the intelligent control system of the far-red light source, but does not limit the protection scope of the present invention.
[0241] The first step is the implementation of the APP client. The matching APP provided by the purchased smart controller manufacturer (see Experimental Materials and Methods for details), and its specific settings and usage methods are detailed in the manufacturer's instruction manual. For th...
Embodiment 2
[0247] Example 2, construction of remote control system elements for gene loops regulating transgene expression by far-red light
[0248] This example includes the construction method of representative elements in the gene loop remote regulation system for regulating the expression of transgenes by far-red light, but it does not limit the protection scope of the present invention. The detailed design scheme and steps are shown in Table 1.
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