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An analytical method for distinguishing chattia honey and syrup adulterated chattia honey

An analysis method, the technology of Vitex honey, is applied in the field of food adulteration identification, which can solve the problems of high modeling cost, inapplicability to daily monitoring, and increased use limitations, and achieve low operational technical requirements and simple pretreatment methods for Vitex honey samples Fast, accurate and reliable results

Active Publication Date: 2020-04-17
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The sensitivity of nuclear magnetic resonance (NMR) is low, and the metabolites we are concerned about in honey may be ignored, and NMR equipment is expensive and not suitable for daily monitoring
Near-infrared spectroscopy (NIRS) also has its fatal shortcomings: 1. It requires a large number of representative samples with known chemical values ​​to build models
Thus, the analysis of small batches of samples using near-infrared becomes impractical
2. The model needs to be updated continuously. Due to the change of the instrument state or the change of the standard sample, the model will also change accordingly.
3. The model is not universal, and the model of each instrument is different, which increases the limitation of use
4. The cost of modeling is high and the cost of testing is high
The various syrups currently adulterated with honey are very similar to honey in terms of physical and chemical properties, composition and content, and flavor. Therefore, the above-mentioned methods for measuring the quality of honey cannot detect honey adulterated with syrup
[0006] To sum up, the existing technology lacks a fast, effective and intuitive method for identifying the adulteration of Vitex twig honey, and there is no combination of ultra-high performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry combined with metabolomics. Studies on Determination of Marker Metabolites in Vitex Honey by Scientific Method

Method used

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  • An analytical method for distinguishing chattia honey and syrup adulterated chattia honey
  • An analytical method for distinguishing chattia honey and syrup adulterated chattia honey
  • An analytical method for distinguishing chattia honey and syrup adulterated chattia honey

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Embodiment 1

[0075] (1) method principle of the present invention:

[0076] After the real Vitex honey sample and the syrup-adulterated Vitex honey sample were treated with the same pretreatment method, UHPLC was used in series with Thermo Fisher Q Exactive Mass Spectrometer to realize the separation and determination of the chemical components in the sample, and the Compounds Discoverer software was used to obtain the original The data were extracted from chromatographic peaks, peak alignment and library search analysis of unknown compounds. Multivariate statistical analysis PCA analysis was used to distinguish real Vitex honey from syrup-adulterated Vitex honey.

[0077] (2) Sample pretreatment

[0078] Weigh 1g of Netia twig honey sample (the number of repetitions for one sample is 6 times) into a 50mL centrifuge tube, add 20mL of extractant (the extractant is mixed with equal volumes of methanol containing 1% formic acid and ultrapure water), and vortex to mix until The honey sample ...

Embodiment 2

[0093] The method for screening the marker metabolites of Vitex twig honey based on UHPLC tandem Thermo Fisher Q Exactive Mass Spectrometer combined with metabolomics method comprises the following steps:

[0094] (1) Sample pretreatment

[0095] Put 1g of Netia twig honey sample in a 50mL centrifuge tube, add 20mL of extractant (the extractant is methanol containing 1% formic acid mixed with ultrapure water in equal volumes), vortex and mix until the Vitex twig honey sample is completely dissolved, and place the centrifuge tube in ultrasonic Sonicate in a washing machine for 25 minutes, centrifuge at 1000 rpm for 5 minutes, and filter the supernatant with a 0.22 μm organic filter membrane for sample loading.

[0096] Put 1g of syrup sample in a 50mL centrifuge tube, add 20mL of extractant (the extractant is mixed with equal volumes of methanol containing 1% formic acid and ultrapure water), vortex and mix until the syrup sample is completely dissolved, and place the centrifug...

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Abstract

The invention discloses an analysis method for identifying vitex chinensis mill honey and syrup adulterated vitex chinensis mill honey, and particularly relates to a method combining the ultra-high performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrum technology with metabonomics. The method comprises the steps that pretreatment is conducted on a true vitex chinensis mill honey sample and a syrup adulterated vitex chinensis mill honey sample through organic solvents respectively, and the ultra-high performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrum technology is used for achieving separation and determination of chemical components in the pretreated samples; then obtained UHPLC-MS original data of the true vitex chinensis mill honey sample and the syrup adulterated vitex chinensis mill honey sample is preprocessed; finally, the multivariate statistical analysis method is used for analyzing and distinguishing the true vitex chinensis mill honey from the syrup adulterated vitex chinensis mill honey. According to the method, metabolite information of the true vitex chinensis mill honey and the syrup adulterated vitex chinensis mill honey is comprehensively obtained, multivariate statistical analysis is used, the vitex chinensis mill honey and the syrup adulterated vitex chinensis mill honey are comprehensively analyzed, and detection of the syrup adulterated vitex chinensis mill honey is completed.

Description

technical field [0001] The invention belongs to the technical field of identification of food adulteration, and in particular relates to an analysis method for identifying vitex honey and syrup adulterated vitex honey by using UHPLC-Q Exactive combined metabonomics technology. Background technique [0002] Honey is the nectar, secretion or honeydew collected by bees from plants, mixed with their own secretions, and fully brewed as a natural sweet substance. Honey has high nutritional value. Among them, Vitex honey is the abbreviation of Vitex flower honey, also called Vitex flower honey. nectar. It is one of the four famous honeys; it is also one of the most stable honey products in my country's bulk honey sources every year. It is not only an ideal nourishing and nutritious food, but also can relieve cough and moisten lungs, invigorate stomach and laxative, prevent heatstroke and clear heart, and has the effect of nourishing body and Qi, and prolonging life. [0003] Howe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14G01N2030/146
Inventor 王骏耿越宿书芳江瑶祝建华王凯利
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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