Kit for screening blood type irregular antibody

Abstract

The invention provides a kit for screening a blood type irregular antibody, and belongs to the technical field of blood type antibody detection. The kit contains six parts of erythrocytes which are packaged separately and divided into three groups; the three groups of erythrocytes come from three persons respectively; at least two of D, C, c, E, e, s, Le, Fy and K antigens in the erythrocytes of each person are positive, and at least one of M, N, S, Le, JK, JK, Fy and P1 antigens is positive; antigen components of the three persons are complementary; each group of erythrocytes consists of two parts of independent erythrocytes packaged in the same specification; one part of erythrocytes is treated with bromelain, and the other part of erythrocytes is not treated with the bromelain; the concentrations of the six parts of erythrocytes in the kit are the same, and can be 1 to 5 percent. The kit is suitable for various blood type antibody detection methods in the prior art. By adopting the kit, the omission ratio of irregular antibodies is reduced, and the clinical blood transfusion risk is lowered.

Description

technical field [0001] The invention relates to the technical field of blood group antibody detection, in particular to a kit for screening blood group irregular antibodies which is applicable to various blood group antibody detection methods. Background technique [0002] In order to prevent the occurrence of various types of transfusion reactions or hemolytic disease of the newborn, it is very necessary to screen relevant blood donors and patients for irregular antibodies. Article 17 of the "Technical Standards for Clinical Blood Transfusion" of the Ministry of Health of my country stipulates that in case of any of the following situations, an antibody screening test must be carried out according to the relevant provisions of the "National Clinical Laboratory Operation Regulations": when the cross-matching blood is not suitable; for those with a history of blood transfusion, pregnancy history or Those who need to receive multiple blood transfusions in a short period of time...

AI Technical Summary

Problems solved by technology

[0005] Since the raw material of the irregular antibody screening kit is human red blood cells, its source is limited, so the antigen spectrum of irregular antibody screening red blood cells produced by different manufacturers is generally flawed, such as Rh, Kidd, Lewis and other blood group systems, most of which are not homozygous , but heterozygous, the homozygous gene encoding produces a double dose of the heterozygous gene, so using heterozygous red blood cells of these blood group systems to detect irregular antibodies may cause weak antibodies against these blood group systems due to dose effects Missed detection; and irregular antibody screening Kidd blood group system and Lewis blood group system on red blood cells have poor affinity with corresponding antibodies, and the agglutination strength is weak, which may also cause irregular antibody detection against Kidd blood group system and Lewis blood group system.

Antibodies against Rh, Kidd, Lewis and other blood group systems account for a relatively large proportion of all irregular antibodies, and most of them have clinical significance. If missed, it may cause more serious immediate or delayed transfusion reactions, endangering the patient's illness

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