MD2 pineapple rooting culture medium and tissue culture seedling rapid propagation method thereof

Abstract

The invention discloses a MD2 pineapple rooting culture medium and a tissue culture seedling rapid propagation method thereof. The MD2 pineapple rooting culture medium contains paclobutrazol. The MD2 pineapple tissue culture seedling rapid propagation method comprises the following steps of: firstly, selecting a MD2 pineapple water sprout and disinfecting the same; and then performing pre-culture, multiplication culture, rooting culture and transplanting thereon, wherein the rooting culture medium is 0.5-1.0mg / L 1 / 2MS+1BA+1.0-2.0mg / L paclobutrazol+20g / L saccharose+6.0-7.0g of agar, and the pH value is 5.5-6.0. The MD2 pineapple seedling is rapidly propagated by way of tissue culture; in the MD2 pineapple seedling rooting culture, after the paclobutrazol is added, the roots are more orderly and more robust, and are better to absorb nutrients; photosynthesis of blades is enhanced, the lobus cardiacus thickness and hardness are increased, water of the blades can be better kept after transplanting, invasion of bacteria and fungi is resisted, the stress resistance of the tissue culture seedling is enhanced, and the transplanting survival rate is also increased to a relatively great extent; rooting culture can be performed by using natural light, so that electric energy is saved; and the rooting seedling cultured under natural lighting can be directly transplanted to a nursery for temporary planting, so that the manual cost is lowered.

Description

technical field [0001] The invention relates to the technical field of pineapple seedling cultivation, in particular to an MD2 pineapple rooting medium and a method for rapid propagation of tissue culture seedlings thereof. Background technique [0002] The weight of MD2 golden pineapple is generally 1-2 kg, cylindrical, with no thorns on the leaf edge, slender leaves with smooth surface, and the number of leaves is 10% more than other varieties, with thin peel and shallow flower cavity. The pulp is golden yellow, fine in texture, medium in fiber, dense in taste, melts in the mouth, rich in flavor and good in taste. In particular, the skin is thin and there is no need to cut the skin, and the sweet taste does not need to be soaked in salt water. It can be eaten directly after peeling, which is very convenient to eat. MD2 golden pineapple is fragrant and juicy, and has a long storage period. If it is stored at 7.5 degrees Celsius, it can be stored for up to 25 days. Therefor...

AI Technical Summary

Problems solved by technology

There are some reports on tissue culture of other pineapple varieties, but due to the large differences in genotypes between different varieties, there are large differences in tissue culture methods between different varieties

MD2 pineapple relies on the traditional vegetative propagation method. Its growth rate is slow and its reproduction coefficient is low. It cannot meet the needs of large-scale planting seedlings in a short period of time. Mother plant pests and diseases passed on to the next generation

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