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Application of AMD3100 to preparation of medicine for treating and/preventing dyscrasia

A cachexia and drug technology, applied in the field of medicine, can solve problems such as undiscovered AMD3100

Active Publication Date: 2017-10-13
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no reports on AMD3100 in the prevention and treatment of dyscrasia have been found

Method used

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  • Application of AMD3100 to preparation of medicine for treating and/preventing dyscrasia
  • Application of AMD3100 to preparation of medicine for treating and/preventing dyscrasia
  • Application of AMD3100 to preparation of medicine for treating and/preventing dyscrasia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 The cultivation of Lewis lung cancer LLC, and the isolation and cultivation of mouse Nestin+ cells

[0045] 1.1 LLC cell culture: LLC cells (ATCC #CRL-1642) were cultured with DMEM basal medium (containing 10% fetal bovine serum) for later use.

[0046] 1.2 Isolation of mouse muscle Nestin+ cells

[0047] Take adult Nestin-GFP transgenic male mice (provided by Dr Masahiro Yamaguchi, and can also be obtained through commercial channels), under sterile conditions, completely excise bilateral gastrocnemius muscles, and quickly place them in a petri dish filled with PBS. Wash the muscle block with PBS 3 times, cut into small pieces of muscle tissue, and remove blood vessels, nerves, and connective tissue as much as possible. Use type I collagenase to digest and separate muscle cells: transfer the muscle tissue to a centrifuge tube, add type I collagenase, and place in a 37°C constant temperature shaking water bath for digestion for 30 minutes. Serum was added...

Embodiment 2

[0052] Example 2 Breeding of mice and construction of tumor cachexia model

[0053] 2.1 Raising method for mice: feed mice with conventional mouse feed (protein content 20-25wt%, fat content 5%-10wt%, crude fiber content 3-5wt%).

[0054] 2.2 Construction of mouse tumor cachexia model

[0055] Male C57BL / 6 mice (purchased from the Institute of Model Animals, Nanjing University) or Nestin-GFP transgenic mice with a weight difference of no more than 2 g at the age of 8 weeks were taken, and the mice were randomly divided into a control group and a dyscrasia group. About 4*10^6 Lewis lung cancer LLC cells were subcutaneously injected into the groin of each cachexia group mouse, and the experiment process was carried out in an ultra-clean bench, paying attention to aseptic operation. The day of inoculation is counted as day 0, then day 1, day 2, and so on. On the 7th day of tumor inoculation, it was detected that the body weight of the tumor-bearing mice began to drop, and tumor...

Embodiment 3

[0056] Example 3 Evaluation of Mice Tumor Cachexia

[0057] The mice in the dyscrasia group in Example 2 were observed at the same time every day after tumor inoculation, coat color, activity, body weight, food intake and tumor size, and compared with those in the control group. Every 2 days to 21 days after tumor inoculation, or on the 7th, 14th, and 21st days, the mice were killed by excessive anesthesia, the tumor tissues were removed, and the tumor-free weights of all animals were weighed; Muscle (QUAD), gastrocnemius (GAST), tibialis anterior (TA), epididymal adipose tissue (eWAT), inguinal adipose tissue (iWAT), and dorsal interscapular brown adipose tissue (iBAT) were weighed.

[0058] figure 1 Middle panel A shows the daily food intake test results of C57BL / 6 mice in the control group and dyscrasia group, panel B is the weight monitoring results of mice in the control group and dyscrasia group; panel C and panel D are adipose tissue and Weight detection of muscle tis...

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PUM

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Abstract

The invention relates to application of AMD3100 or a pharmaceutically acceptable salt of the AMD3100 to preparation of a medicine for treating and / or preventing dyscrasia, and the medicine used for treating and / or preventing dyscrasia.

Description

technical field [0001] The present invention relates to the field of medicine, in particular to the application of AMD3100 or a pharmaceutically acceptable salt thereof in the preparation of medicines for treating and / or preventing tumor dyscrasias, and the medicines for treating and / or preventing tumor dyscrasias. Background technique [0002] Tumor cachexia is a common concomitant symptom of cancer patients, mainly manifested as unintentional weight loss and anorexia. At least 30% of cancer patients died of dyscrasia, and at least 50% of cancer patients died with dyscrasia. The definition of tumor cachexia drafted by Professor Fearon of the School of Clinical Sciences and Community Health, University of Edinburgh, UK, has basically reached a consensus: cachexia is a multifactorial syndrome, manifested by patients who are losing skeletal muscle mass (with or without Loss of fat mass), while traditional nutritional support cannot be completely reversed, which in turn leads ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/395A61P35/00A61P3/00
CPCA61K31/395
Inventor 项鹏汪建成
Owner SUN YAT SEN UNIV
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