Application of brown algae oligosaccharide

A fucoid oligosaccharide and fucoidobiose technology, which is applied in the field of biomedicine, can solve the problems of unsuitable treatment of AKI, complicated pathogenesis, etc., and achieve the effects of preventing the transformation of chronic kidney disease, maintaining kidney function, and reducing expression.

Active Publication Date: 2021-09-28
HAITANG (JIANGSU) BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current treatment of hypertensive nephropathy is mainly based on blood pressure control therapy, however, this therapy is not suitable for the treatment of AKI
Given the complex etiology of AKI, identifying a single therapy that would benefit all AKI patients is challenging

Method used

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  • Application of brown algae oligosaccharide
  • Application of brown algae oligosaccharide
  • Application of brown algae oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1 Preparation and Structure Identification of Fucobiose with Uniform Polymerization Degree

[0080] Dissolve 100 g of purchased alginate (purchased from Qingdao Mingyue Seaweed Group Co., Ltd.) in water, add fucose lyase (obtained from Ocean University of China) at a certain temperature, and centrifuge in a high-speed centrifuge after a certain period of lysis. Take the supernatant. The supernatant was purified with a gel column to remove a small amount of impurity oligosaccharides, polysaccharides and non-sugar impurities to obtain 60 g of fucobiose sodium salt with a purity of more than 95%. Gained fucobiose sodium salt is detected by high performance liquid chromatography (HPLC, 230nm), and is detected by proton nuclear magnetic spectrum ( 1 HNMR) and high-resolution mass spectrometry (HRMS-ESI) for structure identification.

[0081] HPLC: purity 99.06%, RT=13.6min (see the relevant spectrogram figure 1 );

[0082] 1 HNMR spectrum see figure 2 ;

[...

Embodiment 2

[0085] Example 2 Preparation and structure identification of fucoidan with uniform polymerization degree

[0086] Dissolve 100 g of purchased alginate in water, add fucose lyase (obtained from Ocean University of China) at a certain temperature, centrifuge in a high-speed centrifuge after a certain period of time, and take the supernatant. The supernatant was purified with a gel column to remove a small amount of impurity oligosaccharides, polysaccharides and non-sugar impurities to obtain 70 g of fucotriose sodium salt with a purity of more than 95%. Gained fucotriose sodium salt is detected by high performance liquid chromatography (HPLC, 230nm), and is detected by proton nuclear magnetic spectrum ( 1 HNMR) and high-resolution mass spectrometry (HRMS-ESI) for structure identification.

[0087] HPLC: 100% purity, RT=17.43min (see the relevant spectrum Figure 4 );

[0088] 1 HNMR spectrum see Figure 5 ;

[0089] HRMS(ESI)m / z:C 18 h 23 o 18 {(M-H) -}, the calculat...

Embodiment 3

[0091] Example 3 Preparation and Structure Identification of Fucotetraose with Uniform Polymerization Degree

[0092] Dissolve 100 g of purchased alginate in water, add fucose lyase (obtained from Ocean University of China) at a certain temperature, centrifuge in a high-speed centrifuge after a certain period of time, and take the supernatant. The supernatant was purified with a gel column to remove a small amount of impurity oligosaccharides, polysaccharides and non-sugar impurities to obtain 55 g of fucotetraose sodium salt with a purity of more than 95%. Gained fucotetraose sodium salt is detected by high performance liquid chromatography (HPLC, 230nm), and is detected by proton nuclear magnetic spectrum ( 1 HNMR) and high-resolution mass spectrometry (HRMS-ESI) for structure identification.

[0093] HPLC: purity 99.71%, RT=18.71min (see the relevant spectrogram Figure 7 );

[0094] 1 HNMR spectrum see Figure 8 ;

[0095] HRMS (ESI) m / z: C 24 h 31 o 24 {(M-H) ...

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Abstract

The invention provides application of brown algae oligosaccharide or pharmaceutically acceptable salt thereof in preparation of a medicine for treating acute kidney injury, wherein the brown algae oligosaccharide is brown algae disaccharide, brown algae triose and / or brown algae tetraose. The invention also provides application of the brown algae oligosaccharide or the pharmaceutically acceptable salt thereof in preparation of medicines for preventing acute kidney injury from being converted into chronic kidney diseases. After the brown algae oligosaccharide is used for treating an animal with acute kidney injury, the serum creatinine level is obviously reduced, the urine concentration function of the kidney is obviously recovered, the levels of renal tubular injury factors (KIM-1 and NGAL) are obviously reduced, the expression of inflammatory factors is obviously reduced, the pathological change of the kidney is obviously improved, and the treatment effect is enhanced along with the increase of the dosage. Therefore, the brown algae oligosaccharide disclosed by the invention has a relatively strong effect of treating the acute kidney injury. In addition, when acute kidney injury occurs, the brown algae oligosaccharide disclosed by the invention can be used for protecting kidney functions and preventing the acute kidney injury from being converted into chronic kidney diseases.

Description

technical field [0001] The invention relates to an application of fucoidan oligosaccharide, which belongs to the technical field of biomedicine. Background technique [0002] Carbohydrates, together with nucleic acids and proteins, are called the three major living substances. Alginate is mainly found in the cell walls of kelp, sargassum and macroalgae, and is a kind of linear, unbranched, negatively charged polysaccharide compound. Alginate is a binary line composed of β-D-(1,4)-mannuronic acid (M) and α-L-(1,4)-guluronic acid (G) type block compounds. There are mainly three structural fragments in its molecule: Polymannuronate (PM), which is composed of β-D-(1,4)-mannuronic acid interconnected; α-L-(1,4)-ancient Polyguluronic acid (Polyguluronate, PG) formed by interconnecting glucuronic acid; PMG fragment formed by alternating copolymerization of M and G. [0003] The high viscosity and gel-forming properties of alginate make it widely used in food, chemical, pharmace...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7016A61K31/702A61P13/12
CPCA61K31/7016A61K31/702A61P13/12
Inventor 刘振德高河勇
Owner HAITANG (JIANGSU) BIOTECHNOLOGY CO LTD
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