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Biological preparation for remedying oily soil and remediation method and application thereof

A biological agent and soil technology, applied in the field of environmental bioremediation, can solve the problems of inability to meet the needs of efficient degradation of oil-contaminated soil, complex components, etc., and achieve the effect of low cost, simple equipment, and simple process

Active Publication Date: 2017-10-17
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of oil-contaminated soil, a single strain cannot meet the high-efficiency degradation requirements of oil-contaminated soil. Therefore, selecting efficient degradation strains for different components and developing composite bacterial agents are effective ways to improve the degradation efficiency of oil-contaminated soil.

Method used

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  • Biological preparation for remedying oily soil and remediation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method of remediation of oily soil with biological agents is as follows:

[0034] (1) The bacterial agent used in this method is a mixed bacterial agent, which is made by mixing strains a, b, and c according to the ratio of 1:1:1. It is diluted with water, and the liquid medicine ratio is 1 / 10, 1 / 100, 1 / 1000, 1 / 5000 respectively.

[0035] (2) The 10% oily soils used in this method are seaside soil + Gangdong Dazhan oil, Kongdian area soil + Yangsanmu Dazhan oil, Wangguantun soil + Guandazhan crude oil.

[0036] (3) The pH of the 10% oil-bearing soil used in this method is the original pH: the original pH of the border area soil + Gangdong Dazhan oil is 8.31, the original pH of the Kongdian area soil + Yangsanmu Dazhan oil is 9.22, and the Wangguantun soil + The original pH of the crude oil at Guanda Station is 8.91; the three oil-bearing soils were adjusted to pH 6, 7, and 8, respectively.

[0037] (4) The temperature used in this method is normal temperature and ...

Embodiment 2

[0042] The method of remediation of oily soil with biological agents is as follows:

[0043] (1) The bacterial agent used in this method is a mixed bacterial agent, which is made by mixing strains a, b, and c according to the ratio of 1:1:1. It is diluted with water, and the liquid medicine ratio is 1 / 10, 1 / 100, 1 / 1000, 1 / 5000 respectively.

[0044] (2) The 10% oily soils used in this method are seaside soil + Gangdong Dazhan oil, Kongdian area soil + Yangsanmu Dazhan oil, Wangguantun soil + Guandazhan crude oil.

[0045] (3) The pH of the 10% oil-bearing soil used in this method is the original pH: the original pH of the border area soil + Gangdong Dazhan oil is 8.31, the original pH of the Kongdian area soil + Yangsanmu Dazhan oil is 9.22, and the Wangguantun soil + The original pH of the crude oil at Guanda Station is 8.91; the three oil-bearing soils were adjusted to pH 6, 7, and 8, respectively.

[0046] (4) The temperature used in this method is normal temperature and ...

Embodiment 3

[0051] The method of remediation of oily soil with biological agents is as follows:

[0052] (1) The bacterial agent used in this method is a mixed bacterial agent, which is made by mixing strains a, b, and c according to the ratio of 1:1:1. It is diluted with water, and the liquid medicine ratio is 1 / 10, 1 / 100, 1 / 1000, 1 / 5000 respectively.

[0053] (2) The 10% oily soils used in this method are seaside soil + Gangdong Dazhan oil, Kongdian area soil + Yangsanmu Dazhan oil, Wangguantun soil + Guandazhan crude oil.

[0054] (3) The pH of the 10% oil-bearing soil used in this method is the original pH: the original pH of the border area soil + Gangdong Dazhan oil is 8.31, the original pH of the Kongdian area soil + Yangsanmu Dazhan oil is 9.22, and the Wangguantun soil + The original pH of the crude oil at Guanda Station is 8.91; the three oil-bearing soils were adjusted to pH 6, 7, and 8, respectively.

[0055] (4) The temperature used in this method is normal temperature and ...

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Abstract

The invention discloses a biological preparation for remedying oily soil and a remediation method and application thereof. Soil from three different regions is mixed with three kinds of oil to form oily soil with the oil content of 10%, soil pH is detected, and if the soil pH is alkaline, the soil pH is adjusted to 6-8. The biological preparation is prepared by mixing microbial strains a, b and c according to a bacterial count ratio of 1: 1: 1. The biological preparation is diluted by using water according to different proportions, and at a room temperature and at the temperature of 40 DEG C, the oily soil with the pH value of 6-8 and the original pH is treated. After treatment for 48 hours to 4 weeks, the oil content of a soil sample is detected. The remediation method is simple in process, simple in required equipment, low in cost and environment-friendly; the biological preparation is high in oily soil remedying efficiency and can achieve efficient oil-lowering rate within a relatively short time. The remediation method is an efficient method for remedying the oily soil by using the biological preparation, and has a very good application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental bioremediation, and relates to a method and application for remediating oily soil with biological preparations. Background technique [0002] In the process of oil extraction, refining, storage and transportation, a large amount of oil-contaminated soil is produced, including drilling mud, landed oil sludge, and tank cleaning sludge. Oil-polluted soil has very complex components, which contain a large amount of toxic and carcinogenic substances such as benzene, phenol, anthracene, pyrene, etc., which will seriously pollute the environment and endanger human health. Therefore, it is necessary to develop effective and harmless treatment methods. [0003] At present, the technologies for treating oil-contaminated soil can be roughly divided into three categories: physical treatment methods, chemical treatment methods, and biological treatment methods. In the actual processing process, the com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/20
Inventor 王威张丽丽郭姝怡
Owner NANKAI UNIV
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