Method of degrading petroleum hydrocarbon

A technology for petroleum hydrocarbons and petroleum, which is applied in the field of anaerobic degradation of petroleum hydrocarbons for the screening of methanogenic bacteria, achieving the effects of wide application range, high efficiency, and enhanced processing capacity

Pending Publication Date: 2017-07-07
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there is no simple and effec

Method used

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Examples

Experimental program
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Embodiment 1

[0038] A method for degrading petroleum hydrocarbons, comprising the steps of:

[0039] S1. Sampling: collect oilfield production fluid and oily sludge from different areas of the oilfield as samples;

[0040] In this step, six oil well production fluids in an oil field block were collected for research and sampling, and several plastic barrels (about 5L) were filled, and nitrogen was sealed before sampling, and the samples were quickly transported back to the laboratory, stored at low temperature, and numbered They are YH1-YH6; take 4 parts of oily sludge polluted by oil with an oil content of 5.8-11.3% below 30cm from the ground, and store them anaerobically at -5°C after sampling, and they are respectively numbered TH1-TH6; among them, YH1- The physical and chemical properties of YH6 are shown in Table 1.

[0041] Table 1: Physicochemical properties of YH1-YH6

[0042]

[0043] Note 1: YH stands for anaerobic culture oilfield production fluid, and the number behind it ...

Embodiment 2

[0080] On the basis of Example 1, the added concentrations of rhamnolipid, SPAN 20, sodium bicarbonate, sodium acetate, sodium molybdate, zinc chloride, sodium sulfate and sodium nitrate described in S6 were changed to be 80mg / L , 3mg / L, 0.8%, 0.9%, 5mg / L, 50mg / L, 600mg / L and 2600mg / L. Other culture conditions remained unchanged. The final methane gas production using different growth activators is shown in Table 4.

Embodiment 3

[0082] On the basis of Example 1, the added concentration of rhamnolipid, SPAN 20, sodium bicarbonate, sodium acetate, sodium molybdate, zinc chloride, sodium sulfate and sodium nitrate described in S6 was changed to be 40 mg / L , 1mg / L, 1%, 0.8%, 4mg / L, 40mg / L, 900mg / L and 4000mg / L. Other culture conditions remained unchanged. The final methane gas production using different growth activators is shown in Table 4.

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Abstract

The invention provides a method of degrading petroleum hydrocarbon. The method comprises the steps that firstly, petroleum hydrocarbon degradation mixed bacteria flora is extracted in petroleum hydrocarbon, after the petroleum hydrocarbon mixed bacteria flora which can be degraded efficiently is obtained, and a growth activator is added into culture solution of the bacteria flora so that processing ability of the bacteria flora can be strengthened; according to the culture solution used for processing petroleum hydrocarbon, the method has the advantages that the application range is wide and the efficiency of oil degrading is high.

Description

technical field [0001] The invention belongs to the technical field of residual petroleum utilization, and in particular relates to a screening method for anaerobically degrading petroleum hydrocarbon methanogenic bacteria. Background technique [0002] Deep development of old oilfields and enhanced oil recovery have become the central task of the development of old oilfields. The main problem of the existing EOR technology is that the range of enhancement is limited, and a large amount of residual oil remains underground. And how to use a large amount of oil remaining in the oil reservoir has become an important issue. It has become an effective way to utilize residual oil through microbial degradation of oil to produce methane. The process of microbial anaerobic degradation of petroleum to produce methane is a multi-step reaction involving the participation of various bacterial groups. During the entire degradation process, there are many factors that affect the reaction...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12N1/38C12P5/02
CPCC12N1/02C12N1/38C12P5/023Y02E50/30
Inventor 胡恒宇
Owner LINYI UNIVERSITY
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