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Apio americana medikus leaf flavone extract, preparation method and applications thereof

The technology of leaf flavonoids and extracts is applied in the field of soybean taro leaf flavonoids extracts and preparation thereof, and can solve the problems of not finding hypoglycemic activity, failure to develop and utilize resources, waste of resources, etc. The effect of improving hypoglycemic activity

Active Publication Date: 2017-10-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the extraction method and hypoglycemic activity of flavonoids from the leaves of American bean taro
In addition, American bean taro leaves are basically in a waste state at present, and have not been developed and utilized, resulting in a waste of resources

Method used

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  • Apio americana medikus leaf flavone extract, preparation method and applications thereof
  • Apio americana medikus leaf flavone extract, preparation method and applications thereof
  • Apio americana medikus leaf flavone extract, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of bean taro leaf flavonoids extract, the specific steps are as follows:

[0046] (1) Get the American taro leaf, dry, pulverize, and cross a 20-mesh sieve to obtain the taro leaf powder;

[0047] (2) Get 10g of the American bean taro leaf powder obtained in step (1), add 300mL of food-grade ethanol aqueous solution with a volume fraction of 60% to it, and ultrasonically treat it at 30°C and 180W frequency for 30min; Set aside for 18h; after standing still, refrigerate and centrifuge at 4°C, 4000rpm for 30min, and collect the supernatant;

[0048] (3) Take the supernatant of step (2), concentrate under reduced pressure to 50 mL at 40°C, reclaim ethanol, and obtain a concentrated solution;

[0049] (4) The concentrated solution obtained in step (3) is placed in a macroporous resin (AB-8 macroporous resin) for separation, and is eluted with an aqueous ethanol solution with a food grade volume fraction of 60%, and the yellow liquid is collected and st...

Embodiment 2

[0062] This embodiment is except that the following content is different from embodiment 1, and all the other contents are identical to embodiment 1; specifically:

[0063] In step (2), the volume fraction of ethanol aqueous solution is changed into 55% by 60%; The frequency of ultrasonic treatment is changed into 150W by 180W, and the time is changed into 20min by 30min; for 25min.

[0064] The quality of the flavonoids extract of the obtained American beetroot leaf was 138mg.

[0065] The content of total flavonoids in the flavonoids extract of the American taro leaves prepared in this example was measured, and the result was that every 1 g of the flavonoid extracts of the American taro leaves contained 769 mg equivalent of rutin.

[0066] According to HPLC detection, the mass percentages of vitexin and schaftoside in the flavonoids extract of American bean taro leaves prepared in the example are 45% and 19%, respectively.

Embodiment 3

[0068] This embodiment is except that the following content is different from embodiment 1, and all the other contents are identical to embodiment 1; specifically:

[0069] In step (2), the volume fraction of aqueous ethanol is changed from 60% to 65%, and the volume is changed from 300mL to 250mL; the time of ultrasonic treatment is changed from 30min to 35min; in step (3), the concentrated volume is changed from 50mL to 40mL.

[0070] The quality of the flavonoids extract of the obtained American bean jelly leaf was 143mg.

[0071] The content of total flavonoids in the flavonoids extract of American bean leaf flavonoids prepared in this example was measured, and the result was: every 1 g of American bean taro leaf flavonoid extracts contained 782 mg equivalent of rutin.

[0072] As detected by HPLC, the mass percentages of vitexin and schafutasides in the flavonoid extract of American bean taro leaves prepared in the example are 44% and 20%, respectively.

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Abstract

The invention discloses an Apio americana medikus leaf flavone extract, a preparation method and applications thereof, wherein the Apio americana medikus leaf flavone extract contains 78-87% by mass of total flavonoids, the total flavonoids contain vitexin and schaftoside, and by using the Apio americana medikus leaf flavone extract as the reference, the mass fraction of the vitexin is 45-50%, and the mass fraction of the schaftoside is 19-24%. According to the present invention, by using the Apio americana medikus leaf as the object to extract the flavone substances, the obtained Apio americana medikus leaf flavone extract is rich in flavones, especially vitexin and schaftoside, can effectively inhibit the activity of alpha-glucosidase, can promote the carbohydrate metabolism of cells, and has blood glucose lowering activity.

Description

technical field [0001] The invention relates to the technical field of functional foods, in particular to an extract of flavonoids from bean and taro leaves, a preparation method and application thereof. Background technique [0002] American taro (Apio americana Medikus) is a perennial leguminous plant, native to the western United States, and its edible part is underground tubers. It is a natural high-protein, high-calcium food. As early as the 1980s, Japan successfully developed American bean taro as a health product and promoted it to the market. my country also introduced American bean taro into planting in 2009, and it has been well cultivated and promoted in some areas of Zhejiang Province. Many studies have shown that American yam can significantly improve chronic diseases such as hypertension, diabetes and hyperlipidemia. [0003] American taro is mostly used in the development of health food as the tuber part, and there are few reports on its aerial parts such as ...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61K31/352A61K31/7048A61P3/10
CPCA61K31/352A61K31/7048A61K36/48A61K2236/333A61K2236/55A61K2300/00
Inventor 郑晓冬阎芙洁俞露霜杨芸芸
Owner ZHEJIANG UNIV
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