Bacterium for inhibiting growth of Gambierdiscus sp. and application thereof

A technology of algal growth and sub-algae, applied in the application, bacteria, plant growth regulators and other directions, can solve the problems of difficult treatment, difficult metabolic clearance and other problems, and achieve the effect of reducing the secretion of ciguatoxin

Pending Publication Date: 2017-10-24
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since ciguatoxin is a fat-soluble toxin, it is difficult to metabolize and remove it in the body, so it is very difficult to treat

Method used

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  • Bacterium for inhibiting growth of Gambierdiscus sp. and application thereof
  • Bacterium for inhibiting growth of Gambierdiscus sp. and application thereof
  • Bacterium for inhibiting growth of Gambierdiscus sp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1, isolation and identification of bacterial strain XZX-S5

[0066] 1. Isolation, cultivation and screening of algae cell symbiotic bacteria

[0067] Aseptically take out Gambierdiscus sp.1112M1M03, shake it and let it stand for a while (3-5 minutes), take the supernatant and dilute it in multiples, and spread it on a clean bench to obtain cultureable bacterial single clones. After the selected monoclonal strains were cultured, they were co-cultured with Gambierdiscus sp.1112M1M03. After cultivation, the number of algal cells, chlorophyll content and photosynthetic efficiency were detected, and the strains that inhibited the growth of Gambierdiscus were marked as potential inhibitors. Algae strains, and select the algae-inhibiting strain with the most obvious inhibitory effect, and name it XZX-S5.

[0068] 2. Identification of XZX-S5 strain

[0069] 1. Morphological identification

[0070] Describe the single colony state of the XZX-S5 strain isolated in t...

Embodiment 2

[0080] Embodiment 2, gamma proteobacterium Gamma proteobacterium XZX-S5 inhibits the growth of algae gambiae

[0081] 1. Preparation of experimental materials and experimental methods

[0082] 1. Preparation of experimental materials

[0083] The present invention selects Gambierdiscus sp.1112M1M03, which produces strong ciguatera toxin, as an experimental object, and studies the inhibitory effect of Gamma proteobacterium XZX-S5 on the growth of Gambierdiscus.

[0084] (1) Cultivation of Gambierdiscus sp.1112M1M03

[0085] Use a micropipette to draw 10 μL of Gambierdiscus sp.1112M1M03 algae liquid onto a glass slide, and observe the individual conditions of Gambierdiscus sp.1112M1M03 under a 40X optical microscope. After confirming that the growth state is good, transfer 20ml of Gambiae algae liquid to 100mL of fresh K medium medium for expansion. Algal cell concentrations were counted every two days. The counting method is as follows: Gently shake the Erlenmeyer flask of ...

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Abstract

The invention discloses a bacterium for inhibiting growth of Gambierdiscus sp. and application thereof. The bacterial strain provided herein is Gamma proteobacterium XZX-S5 collected under GDMCC No. 60157. The bacterium has the advantages that the Gamma proteobacterium XZX-S5 is screened from the environment, is a symbiotic bacterium to Gambierdiscus sp., and can be used without causing environmental burden or secondary pollution to ecology; the Gamma proteobacterium XZX-S5 not only can inhibit the biomass of Gambierdiscus sp., but has the potential of decreasing secretion of ciguatoxin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a bacterial strain related to the growth inhibition of algae, in particular to a Gamma proteobacterium XZX-S5 which inhibits the growth of Alga gambiae and its application. Background technique [0002] Ciguatera fish poisoning (CFP) is a fat-soluble, highly ether-like substance with very strong toxicity. It is one of the most harmful red tide biotoxins known. It is colorless, odorless, fat-soluble, and insoluble in water. , heat-resistant, not easy to be destroyed by gastric acid, mainly produced by benthic Gambierdiscus sp. The toxin has a food chain cumulative effect. After the toxin is produced by gambiae, it is ingested by fish and enriched in the fish, and finally threatens human health through the food chain. With the expansion of the scale of global fishery trade, poisoning incidents are increasing year by year. Because ciguatoxin is a fat-soluble toxin, it is difficult to me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P13/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 周进谢紫欣尹三军马振华谢思思王洪娟
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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