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Method for extracting protopanoxadiol and metabolic products thereof in biological sample

A protopanaxadiol and biological sample technology, applied in the field of analytical chemistry, can solve the problems of high toxicity of reagents, narrow extraction range, inability to remove small molecular compounds, etc., to achieve the effect of wide applicability and improved detection sensitivity

Inactive Publication Date: 2017-10-31
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the protein precipitation method has the advantages of simple operation and short time consumption, but the disadvantage is that it cannot remove small molecular compounds such as inorganic salts in the sample, and greatly reduces the sample concentration; the advantage of the liquid-liquid extraction method is that the reagents and devices used are cheap and easy to use. Therefore, the impurity removal effect is remarkable, but the disadvantages are that the reagents used are highly toxic and the extraction range is narrow; the advantages of solid phase extraction are that the impurity removal effect is obvious, and it is friendly to the environment, but there are disadvantages such as expensive consumables and many steps

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  • Method for extracting protopanoxadiol and metabolic products thereof in biological sample
  • Method for extracting protopanoxadiol and metabolic products thereof in biological sample

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Embodiment 1

[0020] 1. Experimental drug regimen

[0021] 1.1 Preparation of PPD suspension

[0022] Take 0.5 g of sodium carboxymethylcellulose and add 100 mL of deionized water, heat and stir in a water bath at 40°C until it is completely dissolved, take 500 mg of PPD raw material, add 100 mL of the above 0.5% sodium carboxymethylcellulose solution, Mix well and store at 4°C until use;

[0023] 1.2 Administration and sample collection

[0024] Take 10 male S.D. rats, each with a body weight of 250-300 g. After one week of adaptive feeding, 100 mg / kg body weight was given by intragastric administration at 10:00 the next morning, and venous blood was collected at 13:00 on the same day. , centrifuge to take plasma for later use;

[0025] 2. Sample preparation and analysis

[0026] 2.1 Sample pretreatment

[0027] Take 100 μL of the above rat plasma into a 1.5 mL centrifuge tube, add 1 mg of C8 alkyl-modified magnetic mesoporous material, vortex for 10 min, and use a magnet to attract C...

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Abstract

The invention belongs to the field of analytical chemistry, relates to a method for extracting protopanoxadiol and in-vivo metabolic products thereof in a biological sample, particularly discloses a method for extracting protopanoxadiol and metabolic products thereof in rat plasma based on a C8 alkyl modified magnetic mesoporous material. The method comprises the following steps: by taking the C8 alkyl modified magnetic mesoporous material as a carrier, extracting the protopanoxadiol and in-vivo metabolic products thereof in the biological sample, and detecting the extract by subsequent liquid chromatography-mass spectrometry. The method is simple, convenient, environment-friendly, economic and applicable to the pretreatment process of liquid chromatography-mass spectrometry for the protopanoxadiol and metabolic products thereof in the rat plasma.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for extracting compounds, in particular to a method for extracting the original panaxadiol and its metabolites in a biological sample. In particular, it relates to a method for extracting protopanaxadiol and its metabolites in rat plasma based on a C8 alkyl-modified magnetic mesoporous material. Background technique [0002] As is well known in the art, liquid mass spectrometry is one of the main means for detecting drugs and their metabolites in vivo. When using liquid chromatography-mass spectrometry to detect biological samples, the sample must first be pre-treated to remove proteins, inorganic salts and other impurities that interfere with separation and detection. Currently commonly used pretreatment methods include: protein precipitation, liquid-liquid extraction, and solid-phase extraction. Among them, the protein precipitation method has the advantages of simp...

Claims

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Application Information

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IPC IPC(8): G01N30/08G01N30/02
CPCG01N30/08G01N30/02G01N2030/027
Inventor 段更利李嫣郁颖佳凌今冯嘉楠
Owner FUDAN UNIV