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Method for extracting phycoerythrin from dried porphyra yezoensis

A technology of laver and phycoerythrin, applied in chemical instruments and methods, algae/moss peptides, peptide sources, etc., can solve problems such as less research on phycoerythrin extraction

Inactive Publication Date: 2017-11-03
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current research focuses on the extraction of phycoerythrin from fresh laver, and there are few studies on the extraction of phycoerythrin from dried laver.

Method used

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  • Method for extracting phycoerythrin from dried porphyra yezoensis
  • Method for extracting phycoerythrin from dried porphyra yezoensis
  • Method for extracting phycoerythrin from dried porphyra yezoensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Take 10g of dried seaweed, pulverize with a pulverizer, pass through an 80-mesh sieve, and set aside.

[0030] 2) Take 0.5 g of Porphyra lavera powder, add 15 mL of 0.1M phosphate buffer (pH 7.0) to suspend, freeze at -20°C overnight, and then thaw at room temperature as a freeze-thaw process, repeated three times. Then, under the ice bath, the cells were disrupted with a power of 600 W, and the cells were treated by ultrasonic for 10 s and intermittently for 10 s for 2 min. The obtained algal body broken liquid was centrifuged at 8000g for 15min, the centrifugation was repeated twice, and the supernatant was collected;

[0031] 3) The crude phycobiliprotein extract was precipitated with 50% (w / v) ammonium sulfate and allowed to stand overnight. Centrifuge at 8000g for 15 minutes at 4°C, and discard the supernatant. Add water to dissolve the precipitate, dialyze (cutoff 3500Da), remove ammonium sulfate, dialyze freeze-drying, and obtain phycoerythrin.

[0032] The...

Embodiment 2

[0051] 1) Take 10g of dried seaweed, pulverize with a pulverizer, pass through an 80-mesh sieve, and set aside.

[0052]2) Take 0.5 g of Porphyra lavera powder, add 15 mL of 0.1M phosphate buffer (pH 7.0) to suspend, freeze at -20°C overnight, and then thaw at room temperature as a freeze-thaw process, repeated three times. Under the ice bath, the cells were disrupted with a power of 600 W, and the cells were treated by ultrasonic for 10 s and 10 s intermittently for 2 min. The obtained algal body broken liquid was centrifuged at 8000g for 15min, the centrifugation was repeated twice, and the supernatant was collected;

[0053] 3) The crude phycobiliprotein extract was precipitated with 50% (w / v) ammonium sulfate and allowed to stand overnight. Centrifuge at 8000g for 15 minutes at 4°C, and discard the supernatant. Add water to dissolve the precipitate, dialyze (cutoff 3500Da), remove ammonium sulfate, dialyze freeze-drying, and obtain phycoerythrin.

[0054] The obtained p...

Embodiment 3

[0056] 1) Take 10g of dried seaweed, pulverize with a pulverizer, pass through an 80-mesh sieve, and set aside.

[0057] 2) Take 0.5 g of Porphyra lavera powder, add 15 mL of 0.1M phosphate buffer (pH 7.0) to suspend, freeze at -20°C overnight, and then thaw at room temperature as a freeze-thaw process, repeated three times. Under the ice bath, the cells were disrupted with a power of 600 W, and the cells were treated by ultrasonic for 10 s and 10 s intermittently for 2 min. The obtained algal body broken liquid was centrifuged at 8000g for 15min, the centrifugation was repeated twice, and the supernatant was collected;

[0058] 3) The crude phycobiliprotein extract was precipitated with 50% (w / v) ammonium sulfate and allowed to stand overnight. Centrifuge at 8000g for 15 minutes at 4°C, and discard the supernatant. Add water to dissolve the precipitate, dialyze (cutoff 3500Da), remove ammonium sulfate, dialyze freeze-drying, and obtain phycoerythrin.

[0059] The obtained ...

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Abstract

The invention belongs to the technical field of marine bioactive substancepreparation and specifically relates to a method for extracting phycoerythrin from dried porphyra yezoensis. The method specifically comprises the steps of smashing the dried porphyra yezoensis, screening by using a 80-mesh screen; mixing porphyra yezoensis powder with a phosphate buffer according to a certain ratio, and breaking the cells of the porphyra yezoensis by using a repetitive freeze-thaw method and an ultrasonic method; centrifuging a crude extract, collecting liquid supernatant, adding ammonium sulfate for sedimentation, and centrifuging again to obtain the phycoerythrin. The extracting method provided by the invention has the advantages of simple operation, mild preparation condition, environment friendliness, and high purity and good stability of the obtained phycoerythrin.

Description

technical field [0001] The invention belongs to the technical field of preparation of marine biological active substances, and in particular relates to a method for extracting phycoerythrin from a dried product of laver. Background technique [0002] Phycoerythrin is an important light-harvesting pigment protein of many seaweeds, mainly found in red algae and some cyanobacteria. The subunits of oligomeric proteins are α, β, γ, etc. Usually, the phycoerythrin in red algae is (αβ) 6 It exists in the form of γ, and the pigment molecules bound to the cysteine ​​residue of the subunit are phycoerythrin and phycourobilin. Phycoerythrin has strong absorption in the wavelength range of 480-570 nm in the visible light region, and can produce strong fluorescence. [0003] After extraction and purification, phycoerythrin becomes a product with high added value, which can be widely used in the fields of food, cosmetics, biomedicine, and detection agents. Studies have shown that phycoe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C07K1/34
CPCC07K14/405
Inventor 邢荣娥张恩惠王金霞杨皓月徐超杰刘松李鹏程
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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