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Method for extracting SOD (super oxide dismutase) from bovine blood

A bovine blood and membrane-dissolving technology, applied in biochemical equipment and methods, enzymes, oxidoreductases, etc., can solve the problems of long time, complex extraction process, high cost, and achieve improved extraction rate, simple process and stable structure. Effect

Inactive Publication Date: 2017-11-07
NANNING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for extracting SOD from animal blood, among which patent CN201010241815.0 discloses a large-scale industrialized method for extracting superoxide dismutase from cattle and sheep blood. Coagulation, hypotonic hemolysis, ultrafiltration, rapid heating and cooling through a mixer with heat source / cold source, precipitation, 3-level temperature treatment: first level: 45-55°C; second level: 55-60°C; third level : 60-65 ℃, salting out, ultrafiltration and concentration, freeze-drying and other production steps to obtain SOD with high purity, but the extraction process is too complicated, time-consuming and costly

Method used

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  • Method for extracting SOD (super oxide dismutase) from bovine blood
  • Method for extracting SOD (super oxide dismutase) from bovine blood

Examples

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Effect test

Embodiment 1

[0026] A method for extracting SOD from bovine blood, comprising the following steps:

[0027] (1) Pretreatment: take fresh bovine blood, add anticoagulant, stir and break, and centrifuge to obtain red blood cells;

[0028] (2) Cleaning: wash the red blood cells with 0.1% normal saline, and wash once;

[0029] (3) Hemolysis: After washing, add a film-dissolving agent, adjust the temperature and stir, and then put it into ultrasonic treatment for 20 seconds to obtain hemolysis;

[0030] (4) Heating: Add copper chloride and zinc chloride to the hemolysis, stir evenly, and obtain a crude extract after centrifugation;

[0031] (5) One-time centrifugation: add 0.3 times volume of chloroform-ethanol to the crude extract, mix and stir, and centrifuge to discard the precipitate to obtain the crude enzyme solution;

[0032] (6) Secondary centrifugation: add 0.6 times the volume of solvent to the crude enzyme solution, and centrifuge to get the supernatant;

[0033] (7) Freeze-drying...

Embodiment 2

[0042] A method for extracting SOD from bovine blood, comprising the following steps:

[0043] (1) Pretreatment: take fresh bovine blood, add anticoagulant, stir and break, and centrifuge to obtain red blood cells;

[0044] (2) Cleaning: the erythrocytes were washed with 0.5% normal saline for 3 times;

[0045] (3) Hemolysis: After washing, add a film-dissolving agent, adjust the temperature and stir, and then put it into ultrasonic treatment for 30 seconds to obtain hemolysis;

[0046] (4) Heating: Add copper chloride and zinc chloride to the hemolysis, stir evenly, and obtain a crude extract after centrifugation;

[0047] (5) Primary centrifugation: add 0.4 times the volume of chloroform-ethanol to the crude extract, mix and stir, and centrifuge to discard the precipitate to obtain a crude enzyme solution;

[0048] (6) Secondary centrifugation: add 0.8 times the volume of solvent to the crude enzyme solution, and centrifuge to get the supernatant;

[0049] (7) The above s...

Embodiment 3

[0058] A method for extracting SOD from bovine blood, comprising the following steps:

[0059] (1) Pretreatment: take fresh bovine blood, add anticoagulant, stir and break, and centrifuge to obtain red blood cells;

[0060] (2) Cleaning: the erythrocytes were washed with 0.4% normal saline for 3 times;

[0061] (3) Hemolysis: After washing, add a film-dissolving agent, adjust the temperature and stir, and then put it into ultrasonic treatment for 25 seconds to obtain hemolysis;

[0062] (4) Heating: Add copper chloride and zinc chloride to the hemolysis, stir evenly, and obtain a crude extract after centrifugation;

[0063] (5) One-time centrifugation: add 0.3 times volume of chloroform-ethanol to the crude extract, mix and stir, and centrifuge to discard the precipitate to obtain the crude enzyme solution;

[0064] (6) Secondary centrifugation: add 0.7 times the volume of solvent to the crude enzyme solution, and centrifuge to get the supernatant;

[0065] (7) The above su...

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Abstract

The invention belongs to the technical field of natural medicine extraction and particularly relates to a method for extracting SOD (super oxide dismutase) from bovine blood. The SOD of the bovine blood is extracted through six steps including pretreatment, cleaning, blood dissolution, primary centrifugation, secondary centrifugation and freeze drying. The process is simple, the extraction effect is significant and the product purity is high.

Description

technical field [0001] The invention belongs to the technical field of natural medicine extraction, in particular to a method for extracting SOD from bovine blood. Background technique [0002] SOD is the abbreviation of Super Oxide Dismutase, the Chinese name is superoxide dismutase, which is an important antioxidant enzyme in organisms, widely distributed in various organisms, such as animals, plants, microorganisms, etc.; SOD has special physiological activities and is The primary substance for scavenging free radicals, the level of SOD in the body means an intuitive indicator of aging and death; it has been confirmed that there are more than 60 diseases caused by oxygen free radicals, and it can resist and block oxygen free radicals Damage to cells, repair damaged cells in time, and restore cell damage caused by free radicals. Due to the pressure of modern life, environmental pollution, various radiation and excessive exercise will cause a large number of oxygen free ra...

Claims

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Application Information

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IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 谭春萍
Owner NANNING UNIV
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