A method for improving the activity of glutamic acid decarboxylase with d101 macroporous adsorption resin

Abstract

The invention relates to a method for increasing the activity of enterococcus faecium Glutamate Decarboxylase by using D101 macroporous adsorption resin, and belongs to the technical field of biology. According to the method disclosed by the invention, the D101 microporous adsorption resin is used as an enzyme activity accelerator of enterococcus faecium GAD, and a D101 microporous adsorption resin-GAD composite catalytic system is constructed according to a proportion that the mass of the D101 macroporous adsorption resin to the volume of a substrate solution to the volume of an enterococcus faecium bacterial suspension solution or a GAD free enzyme solution is 1 to 1 to 1; when the D101 microporous adsorption resin-GAD composite catalytic system is subjected to reaction in a water bath oscillator of which the temperature is 37 to 43 DEG C at 80r / min or stirring reaction in a stirring tank at a low speed for 24 to 36 hours, the yield of GABA (Gamma-Aminobutyric Acid) can be increased by 18.54 to 149.11 percent; the D101 macroporous adsorption resin is capable of remarkably increasing the activity of the GAD, an exchange adsorption function of the D101 microporous adsorption resin on the GABA is also a purifying process of the GABA, a downstream extracting and purifying technology is simplified, and the production cost is reduced; the method is simple, green and environment-friendly.

Description

technical field [0001] The invention relates to a method for improving glutamic acid decarboxylase activity by using D101 macroporous adsorption resin, which belongs to the field of biotechnology. Background technique [0002] γ-Aminobutyric acid (γ-Aminobutyric acid, GABA) is a non-protein amino acid with 4 carbon atoms. It is the main inhibitory neurotransmitter in the mammalian central nervous system. Antioxidant, anti-inflammatory, anti-cancer, blood pressure lowering, sedative and sleep improving effects have become the ingredients of pharmaceuticals and health products that have attracted much attention. GABA is also an important raw material for the production of bioplastic polyamide 4 due to the formation of 2-pyrrolidone by lactamization. [0003] Microbes grow and multiply quickly, and their production of GABA is not limited by time and space. Therefore, the production of GABA by microbial glutamate decarboxylase (glutamate decarboxylase, GAD, EC4.1.1.15) has attr...

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Problems solved by technology

[0029] The object of the present invention is to provide a method for improving the conversion activity of Enterococcus faecium glutamic acid decarboxylase by D101 macroporous adsorption resin, by using D101 macroporous adsorption resin as auxiliary catalyst, D101 macroporous adsorption resin, containing glutamic acid Decarboxylase Enterococcus faecium cells, buffer and reaction substrate L-glutamic acid or L-glutamic acid monosodium salt constitute a co-catalytic system, and finally realize the improvement of the conversion activity of Enterococcus faecium cell glutamate decarboxylase and increase the γ - The purpose of GABA output is to solve the problem of high cost of biosynthesis of GABA

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