Preparation method of fish skin antifreeze peptide microcapsules and application of fish skin antifreeze peptide microcapsules in surimi products
A technology of surimi products and microcapsules, applied in surimi products, the field of preparation of fish skin antifreeze peptide microcapsules, can solve the problem of inactivation of antifreeze peptides, formation of larger ice crystals, and reduction of gel strength of surimi products and other problems to achieve the effect of solving frost cracking
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Embodiment 1
[0032] (1) Material selection: choose the skin of Spanish mackerel near the coast as the raw material for extracting antifreeze peptides.
[0033] (2) Preparation of antifreeze peptide: use alkaline protease with an enzyme activity of 2.4AU / g to enzymatically hydrolyze for 120 min at pH 8.5, 42°C, and enzyme to substrate mass ratio 1:50. After the enzymatic solution is ultrafiltered and concentrated, the antifreeze peptide extract with antifreeze activity is obtained.
[0034] (3) Adsorption of antifreeze peptide by porous starch: Weigh an appropriate amount of porous starch and the extract of antifreeze peptide in step (2), mix the two according to the wall-core material ratio (mass ratio) of 10:1, add 20mL of distilled water, and stopper Shake at 20°C for 60 minutes, pour out, vacuum filter until there is no water dripping, quickly wash the residual antifreeze peptide on the surface of the porous starch with double distilled water, freeze-dry for 20 hours, crush and sieve to...
Embodiment 2
[0039](1) Material selection: choose the skin of golden thread fish near the coast as the raw material for antifreeze peptide extraction.
[0040] (2) Preparation of antifreeze peptide: use alkaline protease with an enzyme activity of 2.4AU / g to enzymatically hydrolyze for 120 min at pH 8.5, 42°C, and enzyme to substrate mass ratio 1:50. After the enzymatic solution is ultrafiltered and concentrated, the antifreeze peptide extract with antifreeze activity is obtained.
[0041] (3) Adsorption of antifreeze peptide by porous starch: Weigh an appropriate amount of porous starch and the extract of antifreeze peptide in step (2), mix the two according to the wall-core material ratio (mass ratio) of 30:1, add 20mL of distilled water, and stopper Shake at 40°C for 110 minutes, pour out, vacuum filter until there is no water dripping, quickly wash the residual antifreeze peptide on the surface of the porous starch with double distilled water, freeze-dry for 22 hours, crush and sieve t...
Embodiment 3
[0046] (1) Material selection: choose the skin of Spanish mackerel near the coast as the raw material for extracting antifreeze peptides.
[0047] (2) Preparation of antifreeze peptide: use alkaline protease with an enzyme activity of 2.4AU / g to enzymatically hydrolyze for 120 min at pH 8.5, 42°C, and enzyme to substrate mass ratio 1:50. After the enzymatic solution is ultrafiltered and concentrated, the antifreeze peptide extract with antifreeze activity is obtained.
[0048] (3) Adsorption of antifreeze peptide by porous starch: Weigh an appropriate amount of porous starch and step (2) antifreeze peptide extract, mix the two according to the wall-core material ratio (mass ratio) of 50:1, add 20mL of distilled water, and stopper Shake at 60°C for 160 minutes, pour it out, vacuum filter until there is no water dripping, quickly wash the residual antifreeze peptide on the surface of the porous starch with double distilled water, freeze-dry for 24 hours, crush and sieve to obtai...
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