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Compound collagen tissue regeneration membrane and preparation method thereof

A tissue regeneration membrane and collagen technology, applied in prostheses, bandages, surgery, etc., can solve problems such as poor mechanical properties, large deformation, and short degradation time, and achieve the effects of improving strength, reducing foreign body sensation, and avoiding tissue swelling

Active Publication Date: 2017-11-10
BEIJING PAISHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its mechanical properties are poor, the degradation time is too short, and the deformation after water absorption is large

Method used

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  • Compound collagen tissue regeneration membrane and preparation method thereof
  • Compound collagen tissue regeneration membrane and preparation method thereof
  • Compound collagen tissue regeneration membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A double cross-linked collagen tissue regeneration membrane, with collagen as a matrix and cross-linked collagen sponge as a reinforcement. After the two are compounded, another cross-linking is carried out. Its preparation method is:

[0053] Step S1: Preparation of collagen gel:

[0054] Step S1-1: Take pigskin, remove hair and fascia, slice, add delipase, and then rinse in purified water for 1 hour to remove grease and inactivate;

[0055] Step S1-2: adding the degreasing solution obtained in step S1-1 to a citric acid solution with a pH value of 4, adding a mixture of pepsin and papain, and enzymatically hydrolyzing at 25°C for 20 hours;

[0056] Step S1-3: centrifuge the enzymolysis solution obtained in step S1-2, take the supernatant, use sodium hydroxide solution to adjust the pH value to 6, and use potassium sulfate solution to salt out soluble atelocollagen;

[0057] Step S1-4: Put the atelocollagen solution obtained in Step S1-3 into a dialysis bag, seal it...

Embodiment 2

[0067] A composite collagen tissue regeneration membrane, with collagen as a matrix and a cross-linked collagen sponge as a reinforcement. Its preparation method is:

[0068] Step S1: Preparation of collagen gel:

[0069] Step S1-1: take pigskin, remove hair and fascia, slice, add delipase, then rinse in purified water for 24 hours, remove grease, and inactivate;

[0070] Step S1-2: adding the degreasing solution obtained in step S1-1 into a citric acid solution with a pH value of 5, adding a mixture of pepsin and papain, and enzymatically hydrolyzing at 25°C for 20 hours;

[0071] Step S1-3: centrifuge the enzymolysis solution obtained in step S1-2, take the supernatant, use sodium hydroxide solution to adjust the pH value to 6, and use potassium sulfate solution to salt out soluble atelocollagen;

[0072] Step S1-4: Put the atelocollagen solution obtained in Step S1-3 into a dialysis bag, seal it and perform three-layer gradient dialysis. The dialysis time is 3 days, the t...

Embodiment 3

[0081] A double cross-linked collagen tissue regeneration membrane, with collagen as a matrix and cross-linked collagen sponge as a reinforcement. After the two are compounded, another cross-linking is carried out. Its preparation method is:.

[0082] Step S1: Preparation of collagen gel:

[0083] Step S1-1: take cowhide, remove hair and fascia, slice, add delipase, then rinse in purified water for 12 hours, remove grease, and inactivate;

[0084] Step S1-2: adding the degreasing solution obtained in step S1-1 into a citric acid solution with a pH value of 5, adding subtilisin, and enzymatically hydrolyzing at 25° C. for 96 hours;

[0085]Step S1-3: centrifuge the enzymolysis solution obtained in step S1-2, take the supernatant, use a mixed solution of sodium hydroxide and potassium hydroxide to adjust the pH value to 8, and use a mixture of potassium sulfate and potassium nitrate The solution salts out the soluble atelocollagen;

[0086] Step S1-4: Put the atelocollagen s...

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Abstract

The invention relates to a compound collagen tissue regeneration membrane and a preparation method thereof, and belongs to the technical field of medical materials. The compound collagen tissue regeneration membrane is mainly used for guiding tissue regeneration and preventing tissue adhesion. The compound collagen tissue regeneration membrane is a medical membrane material and can be used for medical science departments such as the stomatology department or the neurosurgery department. Collagen or a collagen compound material is used as a matrix, and a cross-linked collagen sponge or a cross-linked collagen compound sponge is used as a reinforcement body. After compounding of the matrix mentioned above and the reinforcement body mentioned above is completed, one time of physical or chemical cross-linking processing can be conducted once, and the obtained product is the dual cross-linked collagen tissue regeneration membrane. The preparation steps of the compound collagen tissue regeneration membrane include the step S1 of preparation of collagens polypeptide gel, the step S2 of preparation of the collagen sponge and the step S3 of preparation of the compound tissue regeneration membrane. The membrane has an excellent anti-rejection immune property and an excellent mechanical property, expansion of the membrane is very small after water absorption, it is avoided that tissue swelling is caused, the membrane can load medicine, and the degradation speed of the membrane is matched with the speed of tissue growth.

Description

technical field [0001] The invention belongs to the technical field of medical materials and is mainly used for guiding tissue regeneration and preventing tissue adhesion. It is a medical film material, which can be used in medical departments such as stomatology or neurosurgery. Background technique [0002] The guided tissue regeneration technique (Guided Tissue Regeneration, GTR), which emerged in the 1980s, refers to the use of surgical methods to place biofilms in the wound area, isolate the surrounding soft tissues to grow towards the bone surface, and form a certain space to induce the membranous cells to grow towards the bone surface. Move and grow and differentiate to achieve regeneration. [0003] During the process of osteogenesis, cells with osteogenic potential will grow to the defect area and migrate slower than the surrounding connective tissue, resulting in the failure of wound healing. GBR (Guided Bone Regeneration) guided bone regeneration technology uses...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L31/14A61L15/32A61L15/64A61L27/24A61L27/58
CPCA61L15/325A61L15/64A61L27/24A61L27/58A61L31/044A61L31/148
Inventor 张自强徐荣荣李欢郭慧敏
Owner BEIJING PAISHENG BIOTECH CO LTD
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