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Method of preparing bioactive feed by step-by-step fermentation

A biologically active, step-by-step fermentation technology, applied in animal feed, animal feed, application, etc., can solve the problems of not being able to achieve the best fermentation effect and differences in fermentation environment requirements, and solve the problem of waste utilization, low cost, and denaturation low degree of effect

Inactive Publication Date: 2017-11-14
山东中阳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, inoculation and fermentation at the same time, the requirements for the best fermentation environment of each strain are different, and the best fermentation effect cannot be achieved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for preparing biologically active feed by step-by-step fermentation comprises the following steps:

[0027] 1) Pretreatment: clean the low-denatured puffed soybean meal produced by the high-temperature soybean meal production line, and then crush the soybean meal to 30 meshes with a grinder;

[0028] 2) Ingredients: Add distilled water to the crushed soybean meal according to the material-to-water ratio of 0.7:1, and soak for 30 minutes;

[0029] 3) Sterilization: Soybean meal after soaking is sterilized by high-pressure steam at 110°C for 15 minutes, and then used after cooling; the soybean molasses is diluted to a soluble solid content of 5% and then sterilized, and then the diluted soybean molasses is divided into culture Base A, medium B and medium C are cooled for later use;

[0030] 4) Strain activation: Inoculate Bacillus subtilis, yeast and lactic acid bacteria into culture medium A, culture medium B and culture medium C respectively, culture Bacillu...

Embodiment 2

[0036] The method for preparing biologically active feed by step-by-step fermentation comprises the following steps:

[0037] 1) Pretreatment: clean the low-denatured puffed soybean meal produced by the high-temperature soybean meal production line, and then crush the soybean meal to 40 meshes with a grinder;

[0038] 2) Ingredients: Add distilled water to the crushed soybean meal according to the material-to-water ratio of 1.2:1, and soak for 40 minutes;

[0039] 3) Sterilization: Soybean meal after soaking is sterilized by high-pressure steam at 130°C for 30 minutes, and then used after cooling; the soybean molasses is diluted to a soluble solid content of 15% and then sterilized, and then the diluted soybean molasses is divided into culture Base A, medium B and medium C are cooled for later use;

[0040] 4) Strain activation: Inoculate Bacillus subtilis, yeast and lactic acid bacteria into culture medium A, culture medium B and culture medium C respectively, culture Bacill...

Embodiment 3

[0046] The method for preparing biologically active feed by step-by-step fermentation comprises the following steps:

[0047] 1) Pretreatment: clean the low-denatured puffed soybean meal produced by the high-temperature soybean meal production line, and then crush the soybean meal to 35 meshes with a grinder;

[0048] 2) Ingredients: Add distilled water to the crushed soybean meal according to the material-to-water ratio of 1.1:1, and soak for 38 minutes;

[0049] 3) Sterilization: Soybean meal after soaking is sterilized by high-pressure steam at 120°C for 20 minutes, and cooled for later use; the soybean molasses is diluted to a soluble solid content of 11% and then sterilized, and then the diluted soybean molasses is divided into culture Base A, medium B and medium C are cooled for later use;

[0050] 4) Strain activation: Inoculate Bacillus subtilis, yeast and lactic acid bacteria into culture medium A, culture medium B and culture medium C respectively, culture Bacillus ...

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Abstract

The present invention discloses a method of preparing a bioactive feed by a step-by-step fermentation and belongs to the technical field of microbial fermentation. The method solves problems that existing bioactive feeds are high in content of anti-nutritional factors, low in protein content and high in production costs. The method comprises the following steps: impurities of soybean meal are removed and the impurity removed soybean meal is crushed; the crushed soybean meal is soaked using distilled water; the soybean meal and a soybean molasses dilution solution are respectively sterilized; bacillus subtilis, yeasts and lactic acid bacteria are respectively inoculated into soybean molasses dilution solution mediums respectively; the bacillus subtilis is inoculated into the sterilized soybean meal and a preliminary anaerobic fermentation is conducted; after the yeasts and lactic acid bacteria are inoculated into the preliminarily fermented soybean meal, an anaerobic fermentation is conducted; and after the fermentation, the soybean meal is subjected to a low temperature drying to be a moisture content of less than or equal to 12%, and the bioactive feed is obtained. The method is suitable for the preparation of the bioactive feed by the step-by-step fermentation.

Description

technical field [0001] The invention relates to a method for preparing biologically active feed through step-by-step fermentation, which belongs to the technical field of microbial fermentation. Background technique [0002] At present, the main source of high-quality feed protein raw materials is fishmeal. Fishmeal resources are scarce. Affected by various factors, the price continues to rise, resulting in an increase in breeding costs. Therefore, it is necessary to seek low-cost, reliable source of plant protein instead. Soybean meal is a by-product of soybean oil extraction. It has high crude protein content, balanced amino acid composition, and rich in various mineral nutrients. It is a high-quality protein source that is widely used in animal husbandry. However, soybean meal contains various anti-nutritional factors such as trypsin inhibitor and soybean antigen protein, which seriously affect the digestion and absorption of soybean meal protein by animals, and easily ca...

Claims

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Application Information

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IPC IPC(8): A23K10/37A23K10/18A23K10/12
CPCA23K10/12A23K10/18A23K10/37Y02P60/87
Inventor 管勇佳李凤盈姜自强杜现祥安玉辉张玉琛
Owner 山东中阳生物科技有限公司
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