Refirming additive and application thereof
An additive and skin-firming technology, applied in skin care preparations, cosmetic preparations, medical preparations containing active ingredients, etc. Skin firming, softness, and adhesion-promoting effect
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[0024] 1. The preparation method of fish skin collagen peptide is as follows:
[0025] Take the washed fish skin and place it in 10 times the amount of pure water, put it in a water bath at 80°C for 30 minutes, filter, and take the filtrate; centrifuge at 4°C for 15 minutes, take the supernatant; dialyze with double distilled water for 4 hours , repeated 3 times, and then placed in a pre-cooled vacuum freeze dryer, freeze-dried to obtain fish skin collagen; according to the ratio of enzyme to substrate 1:10 (ml / mg), the crude enzyme solution and fish skin collagen Mix and place in a 37°C, 100r / min constant temperature horizontal shaker for enzymolysis for 5 hours; heat the enzymolysis solution in a 95°C water bath for 10 minutes to inactivate the enzyme, cool to room temperature, centrifuge to remove denatured macromolecular proteins, and collect The supernatant liquid was chromatographed with ultrapure water at a flow rate of 1 mlmin, and then freeze-dried to obtain fish skin...
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