Plant endophyte capable of degrading PAEs and application of plant endophyte in PAEs polluted soil repair

A plant endophyte and phytoremediation technology, which is applied in the field of plant endophytes to achieve the effects of avoiding crop pollution risks, avoiding pollution risks, and simple operation.

Active Publication Date: 2017-11-21
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as far as we know, there are no patents and literature reports on the application of plant endophytes alone or in combination with plants to remediate PAEs-contaminated soil and reduce crop absorption and accumulation of PAEs, so as to efficiently use contaminated soil to produce safe agricultural products and realize "side production" , while repairing"

Method used

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  • Plant endophyte capable of degrading PAEs and application of plant endophyte in PAEs polluted soil repair
  • Plant endophyte capable of degrading PAEs and application of plant endophyte in PAEs polluted soil repair
  • Plant endophyte capable of degrading PAEs and application of plant endophyte in PAEs polluted soil repair

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Plant samples were collected from the constructed wetland, and the endophyte isolation and screening test was carried out immediately. Conventional endophyte isolation methods were used. After cleaning the collected fresh plant material samples with sterile water, cut them into 1cm-long segments with sterile scissors, and perform surface sterilization in the ultra-clean bench according to the following procedures, 3% H 2 o 2 Rinse for 2.5 minutes, rinse with sterile water for 4 times, rinse with 70% alcohol for 3 times, rinse with sterile water for 3 times, rinse with 3% NaClO solution for 5 minutes, rinse with sterile water for 3 times, and take the sterile water used for the last rinse of plant materials respectively 100 μL and surface-sterilized plant samples were transferred to LB solid medium (peptone 10g, yeast extract 5g, NaCl 10g, agar powder 1.8%, constant volume 1000mL, pH 7.0, sterilized at 121°C) plates and cultured at 30°C for 24h, No microorganisms grow ...

Embodiment 2

[0039] Plant samples were collected from the constructed wetland, and the endophyte isolation and screening test was carried out immediately. After cleaning the collected fresh plant material samples with sterile water, cut them into 1cm-long pieces with sterile scissors, completely immerse the plant material in a stoppered Erlenmeyer flask or a beaker with a cover filled with 150mL-200mL sterile water, and ultrasonically Sterilize for 25 minutes, with a working frequency of 20KHz and a power of 150W; put the plant material in a sterile mortar, add 2g of sterile quartz sand and 10mL of sterile phosphate buffer (pH7.2), grind and pulverize until homogenized, and take Supernatant 0.5mL, inoculated in 100mL inorganic salt liquid medium containing DBP as the only carbon source, 30 ℃, 150rpm, dark shaker culture, every 7 days as a cycle, gradually increase the initial concentration of DBP, 100mg / Gradient acclimatization from L to 800mg / L; -6 Spread it on a plate containing 1000mg...

Embodiment 3

[0041] (1) Identification of strain morphological characteristics: Streak inoculation of strain YJB3 in LB solid medium, place the plate upside down in a constant temperature incubator, and incubate at a constant temperature of 30°C for 12-18 hours, observe the colony shape, the colony is smooth, light yellow, with neat edges, easy to pick Gram staining of the strain, Gram-positive bacteria, using scanning electron microscope observation, the strain is a long bacillus, 1.5-2.0 μm wide, 5.2-7.8 μm long, see figure 1 .

[0042] (2) Physiological and biochemical tests related to bacterial strains

[0043] Methyl red experiment, urease experiment, catalase experiment, sugar fermentation experiment, starch hydrolysis experiment, citrate experiment, H 2 S test, Voges-Proskauer test, indole test, gelatin hydrolysis test and a series of related physiological and biochemical tests, detailed results are shown in Table 1.

[0044] Table 1 The results of physiological and biochemical te...

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Abstract

The invention discloses a plant endophyte capable of degrading PAEs and application of the plant endophyte in PAEs polluted soil repair. The strain of plant endophyte is Bacillus megaterium YJB3 and is deposited in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, on June 28, 2017 with the preservation number CCTCC NO: M 2017389. A YJB3 bacterial liquid is utilized for colonizing the endophyte in the plant rhizosphere or in the body of a plant by means of rhizosphere soil inoculation, spray treatment or seed soaking, or the endophyte is directly added into the PAEs polluted soil, and both above can increase the soil phthalate acid ester pollution repairing efficiency. The plant endophyte is easy to operate, safe and economic, free from introducing secondary pollution to soil environment and capable of avoiding the risk of agricultural product pollution, and is of great significance for production of safe agricultural products by efficiently utilizing regional medium- and low-concentration polluted soil and realization of production while repair.

Description

technical field [0001] The invention belongs to the field of remediation of organic polluted soil, and in particular relates to a plant endophytic bacterium degrading phthalates (PAEs), and an application method capable of remediating PAEs polluted soil individually or in combination with plants. Background technique [0002] The unreasonable use of agricultural materials such as pesticides, chemical fertilizers and agricultural film, and the discharge of industrial "three wastes" continue to release a large amount of PAEs organic pollutants, resulting in increasingly serious farmland soil pollution. PAEs in contaminated soil are absorbed and accumulated by crops, seriously threatening the quality and safety of agricultural products. PAEs are a class of environmental hormone pollutants, which have endocrine disruption, reproductive and developmental toxicity, liver toxicity and "three-induced" effects. Daily dietary intake of agricultural products containing PAEs leads to l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/11
CPCB09C1/10B09C1/105C12N1/205C12R2001/11
Inventor 莫测辉冯乃宪喻娇李彦文赵海明向垒蔡全英李慧吴冰霄
Owner JINAN UNIVERSITY
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