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Preparation method for selenium-rich astaxanthin chlorella powder

A technology of chlorella powder and astaxanthin, applied in the direction of microorganism-based methods, biochemical equipment and methods, mutant preparation, etc., can solve the problems of under-prominent nutritional components and health effects

Inactive Publication Date: 2017-11-24
JINGCHU UNIV OF TECH
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Problems solved by technology

At present, the chlorella products sold in the market mainly include tablets, capsules, oral liquid of chlorella extract, etc., which have certain nutritional functions, but the disadvantage is that the health effects of the nutritional ingredients of the products are not outstanding.

Method used

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Embodiment

[0030] A preparation method of selenium-enriched astaxanthin chlorella powder, comprising the steps of:

[0031] S1, use 60 Co gamma ray repeated irradiation and super mutagen nitrosoguanidine (NTG) were used to select and breed Chlorella algae, and obtained excellent new strains;

[0032] Specific steps: algae mutagenesis procedure: starting algae → 60 Coγ-ray irradiation→NTG mutagenesis treatment→γ-ray repeated irradiation→very good mutagenic algal species.

[0033] γ-ray irradiation treatment: From the perspective of mutation breeding technology, 60 As a kind of ionizing ray, Coγ-ray has high energy, which can cause gene mutation and chromosomal aberration of algae species, and give algae species 60 Coγ-ray treatment is likely to produce mutants that are beneficial to production, so as to select and breed excellent algal strains.

[0034] Filter the algae species in the logarithmic growth phase to obtain algae mud, wash with fresh BG11 culture solution, and then inoculate...

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Abstract

The invention discloses a preparation method for selenium-rich astaxanthin chlorella powder. The method comprises the following steps: selecting fine chlorella strain bred with ultraviolet ray and performing irradiation mutagenic treatment, thereby acquiring a fine new strain; culturing in a creative optimized BG11 culture medium; using a series of parallel 40.0W red light tubes and 1.2m long 28.0W blue light tubes for irradiating the chlorella liquid, continuously culturing till reaching the logarithmic phase and determining D600nm; inducing the chlorella to generate selenium-rich protein; adopting 1.2m long blue light tubes, high luminous intensity, nitrogen starvation, 51.8g / L glucose and 20.0g / L xylose for performing biomass fermentation and inducing a part of astaxanthin; increasing the illumination intensity in steps in a wide inducing stage; adopting jasmonic acid methyl ester, salicylic acid and ethephon for inducing, thereby acquiring astaxanthin selenium chlorella strain; lastly, adopting a creative combined enzymic method and the optimized high-pressure homogeneous wall breaking for acquiring the chlorella powder and adopting novel shrimp head endogenous enzyme for assisting in extracting astaxanthin.

Description

technical field [0001] The invention relates to the field of preparation of astaxanthin chlorella powder, in particular to a preparation method of selenium-enriched astaxanthin chlorella powder. Background technique [0002] Chlorella is a class of unicellular algae belonging to Chlorophyta, Chlorophyceae, and Chlorellaceae. It is widely distributed in nature. The common species mainly include Chlorella vulgaris, Chlorella pyrenoidosa, and Chlorella ellipsoides. The reported astaxanthin-producing chlorella is Chlorella zofingiensis, and the new (new) astaxanthin-producing chlorella strains collected in the field are also included in this study, which is also the originality of this study. Sufficient vitamin content, high protein content, relatively balanced essential amino acids, chlorella extract, DHA, and EPA are its main features. 107.5g of chlorella dry powder is enough to provide a person's daily requirement for all vitamins except vitamin C. [0003] Some provinces an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N15/01C12P21/00C12P23/00C12R1/89
CPCC12N13/00C12N15/01C12P21/00C12P23/00
Inventor 凌善锋孙永剑陆士水陈晗韩佳祥
Owner JINGCHU UNIV OF TECH
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