A kind of breeding method of high-protein peanut variety
A high-protein, peanut technology, applied in the field of plant breeding, can solve the problems of poor seed selection effect, weakened output traits of backcrossing times, etc., and achieve the effect of less blindness, simple method, and clear breeding goals
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Embodiment 1
[0029] Example 1. Obtaining two molecular markers highly correlated with high-protein traits by association analysis method
[0030] 1. 6 high-protein varieties of peanuts (Yingde Baisha chickpeas, Damaling, July beans, Qiongshan beans, Ding'an Dahua, Meihua soybeans), and 6 ordinary protein varieties (AH 66, 80-28②, Huihong 40, G201, ICGV95358, PI381622), provided by the Crop Research Institute of Guangdong Academy of Agricultural Sciences. 80 pairs of primers with good amplification effect and high polymorphism were selected from the SSR primers developed by Knapp et al., and the primers were synthesized.
[0031] 2. DNA extraction. Take the young peanut leaves of the above-mentioned 12 varieties, carry out according to the operating instructions of the Plant Genomic DNA Extraction Kit (spin column type) of Beijing Biotek Biotechnology Co., Ltd., detect by 1% agarose gel electrophoresis, and dilute the sample to 25ngμL- 1. Store at -20°C for later use.
[0032] 3. PCR amp...
Embodiment 2
[0038] Example 2: Using molecular markers GM672 and GM1990 to screen high-protein plants as the female parent for one backcross breeding to obtain high-protein peanut varieties.
[0039] 1. Select a high-yield and disease-resistant peanut variety as the female parent, select a high-protein peanut variety as the male parent, and cross to obtain F1 generation spare seeds.
[0040] 2. Sow a single seed of the F1 generation spare seeds. After the peanut leaves grow, take the young peanut leaves, extract DNA, and amplify with markers GM672 and GM1990. Originally, a high-protein peanut variety was used as the male parent for backcrossing to obtain BC1F1 generation seeds.
[0041] 3. Sow a single seed of BC1F1 spare seeds. After the peanut leaves grow, take the young peanut leaves, extract DNA, amplify with markers GM672 and GM1990, and select peanut plants with high protein content according to the banding results, and self-cross , harvested from a single plant to obtain F2 generatio...
Embodiment 3
[0047] 1. Select the high-yield and disease-resistant peanut variety Yueyou 13 as the female parent, which has the following characteristics: high-yield and disease-resistant. The high-protein peanut variety July Bean was selected as the male parent, which has the following characteristics: the protein content is 40.11%. F1 generation seeds were obtained by crossing.
[0048] 2. F1 generation spare seeds were sown with a single seed, and 21 plants survived. After the peanut leaves grew, the young peanut leaves were taken, DNA was extracted, and the markers GM672 and GM1990 were used to amplify. According to the results of the banding (such as image 3 , Figure 4 ), marker GM672 amplified F1 plants 1, 2, 4, 11, 12, 13, 15, and 17 had bands at 298bp, marker GM1990 amplification was used to select 8 peanut plants with high protein content as female parents, high The protein peanut variety "Jiyuedou" was used as the male parent for backcrossing to obtain BC1F1 generation seeds....
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