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Composite natural bacteriostatic agent as well as preparation method and application thereof
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A natural antibacterial agent, antibacterial agent technology, applied in the direction of application, preservation of meat/fish with chemicals, food ingredients as antioxidants, etc., can solve problems such as poor solubility
Inactive Publication Date: 2017-12-01
WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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Embodiment 1
[0028] The preparation method of oligochitosan-nisin provided by the present embodiment comprises the following steps:
[0029] (1) Oligochitosan and nisin were dissolved in distilled water at a mass ratio of 5:1, the total mass concentration was controlled at 2%, and the pH was adjusted to 2.5 with food-grade HCL.
[0034] The preparation method of oligochitosan-nisin provided by the present embodiment comprises the following steps:
[0035] (1) Oligochitosan and nisin were dissolved in distilled water at a mass ratio of 5:1, the total mass concentration was controlled at 2%, and the pH was adjusted to 2.7 with food-grade HCL.
[0036] (2) Stir the mixed solution at room temperature for 2 hours to ensure full dissolution, and then heat the mixed solution through gradient heating method. The specific process is: heat at 40°C for 8h, then heat up to 50°C for 12h, and continue to heat up to 60°C Heated for 16 hours, then heated to 70°C for 4 hours.
[0037] (3) After heating, the sample was cooled rapidly, then freeze-dried into powder, and stored at -18°C.
[0040] The preparation method of oligochitosan-nisin provided by the present embodiment comprises the following steps:
[0041] (1) Oligochitosan and nisin were dissolved in distilled water at a mass ratio of 5:1, the total mass concentration was controlled at 2%, and the pH was adjusted to 2.3 with food-grade HCL.
[0042] (2) Stir the mixed solution at room temperature for 2 hours to ensure full dissolution, then add biotin, the ratio of the weight of added biotin to nisin is 2:1, and then heat the mixed solution at 60°C for 48 hours.
[0043] (3) After heating, the sample was cooled rapidly, then freeze-dried into powder, and stored at -18°C.
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Abstract
The invention discloses a composite natural bacteriostatic agent prepared through a Maillard reaction, as well as a preparation method and application of the composite natural bacteriostatic agent. The composite natural bacteriostatic agent provided by the invention is a derivative prepared by chitooligosaccharide-nisin through a Maillard reaction. The bacteriostatic agent provided by the invention can be used as an antibacterial agent, a fresh keeping agent and an antioxidant in food service industry. The novel composite natural bacteriostatic agent is synthetized by chitooligosaccharide and nisin through the Maillard reaction. Bacteriostasis rate contrast tests prove that the bacteriostasis capacity of a synthesized derivative after a moderate reaction to escherichia coli is increased by 10 times or above. Fresh keeping tests on small miscellaneous fish prove that a moderate synthetical reactant can effectively prolong the fresh keeping period of the small miscellaneous fish, and can effectively delay the growth of TVB-N and TBA.
Description
technical field [0001] The invention relates to a composite natural bacteriostatic agent, which is a derivative prepared from chitosan-nisin by Maillard reaction, and belongs to the field of biotechnology. Background technique [0002] Nisin, also known as nisin, is a bacteriocin with strong antibacterial effect synthesized and secreted by certain nisin bacteria during the metabolic process, and can be degraded by chymotrypsin in the human body. The harmless amino acid formed in the intestinal tract is a natural biological food preservative and antibacterial agent recognized as safe in the world. However, due to the poor solubility, easy hydrolysis, and reaction with food components (such as proteins and lipids) of nisin when it is used in food, the antibacterial activity of Nisin is reduced and its antibacterial time is shortened. [0003] Chitooligosaccharide (COS) is a low molecular weight basic amino oligosaccharide with a polymerization degree of 2-20 obtained after th...
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