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A kind of osteoinductive composite silk fibroin scaffold for MRI imaging and preparation method thereof

A protein scaffold and silk fibroin technology, which is applied in the field of biomedical engineering materials to achieve the effects of improved resolution, clear magnetic resonance imaging maps, and easy availability of raw materials

Active Publication Date: 2020-07-24
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Based on this, in order to overcome the defects of the above-mentioned existing tissue engineering scaffolds, the present invention provides an osteoinductive composite silk fibroin scaffold for MRI imaging and its preparation method and application

Method used

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  • A kind of osteoinductive composite silk fibroin scaffold for MRI imaging and preparation method thereof
  • A kind of osteoinductive composite silk fibroin scaffold for MRI imaging and preparation method thereof
  • A kind of osteoinductive composite silk fibroin scaffold for MRI imaging and preparation method thereof

Examples

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Embodiment 1

[0035] Example 1 Osteoinductive composite silk fibroin scaffold for MRI imaging and preparation method thereof

[0036] The preparation method of the osteoinductive composite silk fibroin scaffold for MRI imaging of the present embodiment comprises the following steps:

[0037] (1), take 8g silkworm cocoons and place them in a large beaker, add 400ml deionized water, and add 2gNaHCO at the same time 3 Sodium acid, boiled at 100°C for 30 minutes, taken out, washed several times with deionized water until neutral, boiled again and taken out, washed and dried in an oven at 50°C to obtain silk fibroin for use;

[0038](2), take 2.5g silk fibroin and add 25ml 9.3mol / L lithium bromide solution, after heating in a water bath at 60°C for 5h, dialyze with a dialysis bag (the molecular weight cut-off of the dialysis bag is 8kD, dialyze for 3 days, change the water 3 times a day ), and then at 25°C, centrifuged at 5000r / min for 10min, the obtained silk fibroin solution was reverse dialy...

Embodiment 2

[0042] Example 2 Osteoinductive composite silk fibroin scaffold for MRI imaging and its preparation method

[0043] The osteoinductive composite silk fibroin scaffold for MRI imaging of the present embodiment and the preparation method thereof comprise the following steps:

[0044] (1), take 8g silkworm cocoons and place them in a large beaker, add 400ml deionized water, and add 2g NaHCO at the same time 3 , the temperature reached 100°C, boiled for 30 minutes, took it out, washed it several times with deionized water until it was neutral, boiled it again and took it out, washed it and put it in an oven at 50°C to dry to obtain silk fibroin for use;

[0045] (2) Add 2.5g of silk fibroin to 25ml of 9.3mol / L lithium bromide solution, heat it in a water bath at 60°C for 5 hours, then dialyze with a dialysis bag (the molecular weight cut-off of the dialysis bag is 8kD and dialyze for 3 days, change the water 3 times a day) , and then at 25°C, centrifuged at 5000r / min for 10min, t...

Embodiment 3

[0049] Example 3 Osteoinductive composite silk fibroin scaffold for MRI imaging and its preparation method

[0050] The osteoinductive composite silk fibroin scaffold for MRI imaging of the present embodiment and the preparation method thereof comprise the following steps:

[0051] (1), take 8g silkworm cocoons and place them in a large beaker, add 400ml deionized water, and add 2g NaHCO at the same time 3 , the temperature reached 100°C, boiled for 30 minutes, took it out, washed it several times with deionized water until it was neutral, boiled it again and took it out, washed it and put it in an oven at 50°C to dry to obtain silk fibroin for use;

[0052] (2) Add 2.5g of silk fibroin to 25ml of 9.3mol / L lithium bromide solution, heat it in a water bath at 60°C for 5 hours, then dialyze with a dialysis bag (the molecular weight cut-off of the dialysis bag is 8kD and dialyze for 3 days, change the water 3 times a day) , and then at 25°C, centrifuged at 5000r / min for 10min, t...

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Abstract

The invention discloses an osteoinductive composite silk fibroin scaffold for MRI imaging and a preparation method thereof. The volume of the composite silk fibroin scaffold is 1.5-2.0mm 3 , the composite silk fibroin scaffold is loaded with 3×10 5 ~7×10 5 A dental pulp stem cell, the composite silk fibroin scaffold is prepared from a 2-10% silk fibroin solution and a dispersion formed of hydroxyapatite and superparamagnetic iron tetroxide as raw materials. The composite silk fibroin scaffold of the present invention has a suitable pore size and porosity, which is beneficial to the adhesion and proliferation of dental pulp stem cells, overcomes some problems of lack of existing scaffold materials, multiple substances are compounded, and the advantages of the original substance are maintained. It solves the deficiency of the performance of a single original material, the preparation process is simple, and the required raw materials are easy to obtain. The prepared composite scaffold is expected to be widely used in the field of biomaterials; as a marker for tissue engineering scaffolds, superparamagnetic iron tetroxide It is expected to be applied in clinical medicine.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials. Specifically, the invention relates to an osteoinductive composite silk fibroin scaffold for MRI imaging and a preparation method thereof. Background technique [0002] With the development of imaging technology, various functional imaging studies have emerged. Magnetic resonance imaging (MRI) has been effectively used to monitor tissue engineering scaffolds because of its non-invasiveness, safety, and rich diagnostic information. The use of contrast agents can effectively improve the sensitivity and specificity of magnetic resonance diagnosis, enhance the signal contrast and improve the resolution of soft tissue images. Superparamagnetic iron tetroxide is a kind of nanoparticles with paramagnetism and significant negative effect, which has become a research hotspot in the field of magnetic resonance imaging. [0003] Dental pulp stem cells (DPSCs) have stem cell characteristics ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/38A61L27/12A61L27/02A61L27/50A61K49/08
CPCA61K49/08A61L27/025A61L27/12A61L27/227A61L27/3834A61L27/50A61L2300/112A61L2300/252A61L2300/412
Inventor 郭瑞李丹蓝咏
Owner BEOGENE BIOTECH GUANGZHOU
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