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Process for supercritical carbon dioxide fluid extraction of total flavonoids from spina gleditsia

A carbon dioxide and saponin thorn technology, which is applied in the directions of medical preparations containing active ingredients, antidote, plant raw materials, etc., can solve the problems that the extraction technology of total flavonoids cannot be satisfied, and achieves obvious antioxidant properties, simple operation, and extraction rate. high effect

Inactive Publication Date: 2017-12-08
江苏省徐州医药高等职业学校
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction technology of total flavonoids cannot meet the above requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A process for using supercritical carbon dioxide fluid to extract total flavonoids in saponaria spurs specifically includes the following steps:

[0026] 1) Drying: Put the traditional Chinese medicine saponin spines in a vacuum, and dry them at 35°C for 60 hours;

[0027] 2) Crushing: Crush through a 60-mesh sieve and add to the supercritical extraction tank;

[0028] 3) Supercritical extraction: According to the carbon dioxide flow rate per 100g of saponaria thorns is 10L / h, the entraining agent is a mixed solution composed of absolute ethanol and ethyl acetate in a volume ratio of 7:3, the extraction time is 4h, and the extraction temperature is 40℃, the pressure is 15MPa;

[0029] 4) Total flavonoid extract: The extract of step 3) is vacuum-rotated to evaporate the residual ethanol and ethyl acetate to obtain the total flavonoid extract of Saponaria japonicus. The extraction rate of total flavonoids reached 80.7%.

[0030] Take the total flavonoids extract of Saponaria sapo...

Embodiment 2

[0032] A process for using supercritical carbon dioxide fluid to extract total flavonoids in saponaria spurs specifically includes the following steps:

[0033] 1) Drying: Put the traditional Chinese medicine saponin thorns in a vacuum, and dry for 48 hours at 40℃;

[0034] 2) Crushing: crushed through 80 mesh sieve and added to the supercritical extraction tank;

[0035] 3) Supercritical extraction: The flow rate of carbon dioxide used for every 100g of saponin spines is 15L / h, the entrainer is a mixed solution composed of absolute ethanol and ethyl acetate in a volume ratio of 7:3, the extraction time is 6h, and the extraction temperature is 37℃, the pressure is 18MPa;

[0036] 4) Total flavonoid extract: The extract of step 3) is vacuum-rotated to evaporate the residual ethanol and ethyl acetate to obtain the total flavonoid extract of Saponaria japonicus. The extraction rate of total flavonoids is 86.2%.

[0037] Take the total flavonoid extract of Saponaria serrata prepared by th...

Embodiment 3

[0039] A process for using supercritical carbon dioxide fluid to extract total flavonoids in saponaria spurs specifically includes the following steps:

[0040] 1) Drying: Put the traditional Chinese medicine saponin spines in a vacuum, and dry them for 40 hours at 45°C;

[0041] 2) Crushing: crushed through a 100-mesh sieve and added to the supercritical extraction tank;

[0042] 3) Supercritical extraction: According to the carbon dioxide flow rate per 100g of saponaria thorns is 20L / h, the entrainer is a mixed solution composed of absolute ethanol and ethyl acetate in a volume ratio of 7:3, the extraction time is 10h, and the extraction temperature is 30℃, the pressure is 25MPa;

[0043] 4) Total flavonoid extract: The extract of step 3) is vacuum-rotated to evaporate the residual ethanol and ethyl acetate to obtain the total flavonoid extract of Saponaria japonicus. The extraction rate of total flavonoids reached 85.4%.

[0044] Take the total flavonoid extract of Saponaria serrat...

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Abstract

The invention discloses a process for supercritical carbon dioxide fluid extraction of total flavonoids from spina gleditsia, and belongs to the technical field of extraction of total flavonoids. The process comprises the following steps: 1) drying: drying the spina gleditsia, as a traditional Chinese medicine, in a vacuum environment at low temperature; 2) crushing: crushing and sieving the dried spina gleditsia, and adding the processed spina gleditsia to a supercritical extraction tank; 3) implementing supercritical extraction: extracting the spina gleditsia at an extraction temperature of 30-40 DEG C and under a pressure of 15-25MPa for 4-10h by taking a mixed solution that the volume ratio of absolute ethyl alcohol to ethyl acetate is at 7 to 3 as an entrainer, wherein for each 100g of the spina gleditsia, a carbon dioxide flow rate is 10-20L / h; and 4) obtaining a total flavonoid extract: implementing vacuum rotary evaporation on an extract obtained in the step 3) so as to remove residual ethanol and ethyl acetate, so that the total flavonoid extract of the spina gleditsia is obtained. The process provided by the invention, which applies a supercritical CO2 fluid extraction technology to the extraction of the total flavonoids of the spina gleditsia, has the advantages of being simple to operate, free from solvent residue in the finished product (the total flavonoids), high in extraction rate, free from toxins and harm ad the like.

Description

Technical field [0001] The invention relates to a process for extracting the total flavonoids in the saponaria spinosa by using supercritical carbon dioxide fluid, and belongs to the technical field of extraction of total flavonoids. Background technique [0002] Ginsenoside thorn is the dried spines of the leguminous acacia plant. As a traditional Chinese medicine, whether it is a single medicine or a compound medicine, it is very effective and widely used in clinical applications. The 2010 edition of the Pharmacopoeia of the People's Republic of China records that the saponaria thorn has the effects of reducing swelling and supporting toxins, draining pus, and killing insects; it is used for the initial onset of ulcer or pus is not ulcerated; external treatment of scabies and leprosy. Modern pharmacological studies have shown that Saponaria saponaria mainly contains flavonoids, phenols, amino acids, etc., and has antibacterial, immune regulation, and anti-cancer effects. [0003...

Claims

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Application Information

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IPC IPC(8): A61K36/483A61P39/06
CPCA61K36/483A61K2236/37
Inventor 刘晓娟高侠李琼金杰孙蓓曹美玉郭庆宇张新蕊
Owner 江苏省徐州医药高等职业学校
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