A strain of Bacillus Velez gt11 and its application

A technology of Bacillus velesi and Bacillus, applied in the application, bacteria, fungicides and other directions, can solve the problems of inability to obtain disease control effect, soil structure damage, etc., and achieve high-efficiency control effect, good comprehensive properties, and good growth promotion. effect of action

Inactive Publication Date: 2020-07-24
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term application or excessive application of quicklime, in addition to causing damage to the soil structure, in many cases cannot achieve good disease control effects

Method used

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  • A strain of Bacillus Velez gt11 and its application
  • A strain of Bacillus Velez gt11 and its application
  • A strain of Bacillus Velez gt11 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Bacillus velezensis GT11 Aqua

[0029] (1) Bacteria culture

[0030] a. test tube culture (below are weight percentages)

[0031] Put NA medium (5g of peptone, 10g of sucrose, 1g of yeast powder, 3g of beef extract, 17-20g of agar) into a test tube, make up to 1000mL with distilled water, sterilize, make a plate, and inoculate Bacillus velei GT11 Strains, cultivated at 28°C for 48h.

[0032] b. Bacteria Shaker Expansion Cultivation

[0033] The plate seeds were inoculated on the NA medium of the eggplant bottle slant (the medium formula is the same as the above-mentioned step a), and cultured at 26° C. for 48 hours to obtain the eggplant bottle seeds.

[0034] (2) fermentation

[0035] The specific fermentation production process is as follows:

[0036] a. Preparation of seed suspension: wash the colony on the slope of the eggplant bottle with sterilized water to obtain the seed suspension;

[0037]b. Inoculation: the seed suspension is passed throu...

Embodiment 2

[0042] Preparation of Wettable Powder of Bacillus velezensis GT11

[0043] (1) prepare liquid biocontrol agent: with embodiment 1, obtain fermented liquid;

[0044] (2) Add filler: Put the fermentation hydraulic pressure into the storage tank, add diatomite (light calcium carbonate) as filler according to the following calculation formula, and stir for 30 minutes;

[0045] Adding amount of filler (kg)=[number of fermentation broth bacteria (100 million / ml) X tank volume (liter) X yield] / number of finished product bacteria-residue in fermentation broth (kg). Then, it is filtered by plate and frame, beaten, dried, crushed, and made into wettable powder for field use.

[0046] (3) Plate and frame filtration: use 2kg / cm 2 Press the material into the plate frame from the storage tank, filter through No. 7 filter cloth, adjust the pressure at any time, so that the bacteria content in the filtrate is not more than 0.2 billion / ml, and the filter cake and filtrate are obtained;

[0...

Embodiment 3

[0052] In Vitro Antibacterial Test of Bacillus velezensis GT11 on Inchi Fruit Root Rot

[0053] The media used are as follows:

[0054] KB medium (peptone 20g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate heptahydrate 1.5g, glycerol 10mL, distilled water 1000mL; pH7.0);

[0055] PDA medium (potato 200g, agar 15g, glucose 15g, water 1000mL);

[0056] NA medium (peptone 5g, beef extract 3g, yeast extract 1g, sucrose 10g, agar 17g, water 1000mL, pH7.0);

[0057] 1. Bacillus velezensis (Bacillus velezensis) GT11 in vitro antibacterial test on Inki fruit root rot and other pathogenic bacteria

[0058] 1.1 Pathogens tested

[0059] Including Fusarium oxysporum, Fusarium solani, Botrytis cinerea, Cylindrocarpon destructans, Phytophthora parasitica var.nicotianae, etc., the above pathogens Provided by the Plant Pathology Laboratory of Plant Protection College, Yunnan Agricultural University.

[0060] 1.2 Test method

[0061] Activation of pathogenic bacteria: move the ...

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Abstract

The invention discloses a strain of Bacillus Velez GT11 and its application. The Bacillus Velez GT11 has been preserved in the General Microorganism Center of China Committee for the Preservation of Microorganisms on December 13, 2016, and the preservation number is CGMCCNo.13446 The Bacillus Velez GT11 can be made into water, wettable powder or coating agent for the prevention and treatment of Panax notoginseng, Indian root rot and cabbage clubroot. , non-toxic, safe, non-residue, and easy to use biocontrol fungicides for the prevention and treatment of root rot and clubroot. , can degrade the cell wall of pathogenic bacteria, and has a good growth-promoting effect on crops such as Inchi fruit, Panax notoginseng and Chinese cabbage. It has good antibacterial effect on rice bacterial streak disease and so on.

Description

technical field [0001] The invention relates to the technical field of biocontrol agents, in particular to a strain of Bacillus Velez GT11 and its application. Background technique [0002] Sacha inchi (Plukenetia Volubilis., sacha inchi), also known as Sacha inchi, South American oil vine, and American vine fruit, is a woody vine and perennial oil crop belonging to the family Euphorbiaceae. Native to the Amazon River Basin in Peru, South America, this plant has been eaten in the Inca region of South America for thousands of years. Inchi fruit has many health functions such as regulating blood lipids, preventing cardiovascular diseases, and anti-aging. Inchi oil, the content of three kinds of unsaturated fatty acids (α-linolenic acid, linoleic acid and oleic acid) is more than 92%, and the content of α-linolenic acid (43.83%-48.6%) is significantly higher than that of olive oil and tea Seed oil and other edible oils; Sacha Inchi oil also contains lipid active substances (t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 姬广海魏兰芳朱书生付丽娜杨俊汪娅婷王星
Owner YUNNAN AGRICULTURAL UNIVERSITY
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