An In Vivo Test Method for Mitochondrial Membrane Fluidity in the Liver of Osprey carp

Abstract

The invention relates to testing of fluidity of fluidity of a mitochondrial membrane of the liver of fish, in particular to an in-vivo test method for the fluidity of the mitochondrial membrane of the liver of pseudorasbora parva. The method comprises steps as follows: S1, a fish body exposure experiment; S2, preparation of the mitochondrial membrane of the liver of the pseudorasbora parva; S3, preparation of a DPH solution; S4, marking of a DPH fluorescent probe of the mitochondrial membrane; S5, determination of the fluidity of the membrane; S6, determination of the content of protein in mitochondria. With the adoption of the in-vivo test method for the fluidity of the mitochondrial membrane of the liver of pseudorasbora parva, influence of an agent on the fluidity of the mitochondrial membrane of the fish is determined accurately and conveniently.

Description

technical field

[0001] The invention relates to the test of the fluidity of the mitochondrial membrane of the fish liver, in particular to a method for testing the fluidity of the mitochondrial membrane of the wheat ear fish liver in vivo. Background technique

[0002] In recent years, with the continuous development of modern biotechnology, the research methods of the toxicological mechanism of insecticides are also developing towards the molecular level. One of these aspects is the study of biofilm effects. The fluidity of biomembrane phospholipid bilayer is one of the basic characteristics of biomembrane. A large number of studies have shown that the proper fluidity of biofilm is a necessary condition for the normal function of biofilm. For example, material transport, energy conversion, cell recognition and differentiation, cellular immunity, hormone action, membrane-bound enzyme activity, etc., are all closely related to membrane fluidity. An appropriate degree of me...

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