An In Vivo Test Method for Mitochondrial Membrane Fluidity in the Liver of Osprey carp
A testing method, mitochondrial membrane technology, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve problems such as poor biological function, membrane damage, lack of drug absorption, distribution, metabolism and excretion process, and avoid short-term effect, predictable effect
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Embodiment 1
[0049] A method for measuring the fluidity of mitochondrial membranes in the liver of wheat ear carp in vivo, characterized in that it comprises the following steps:
[0050] S1. Fish exposure experiment
[0051] The water body poisoning method was adopted in the exposure experiment of the wheat ear fish body: the wheat ear fish was domesticated for 14 days indoors, and the mortality rate was stabilized below 5%, and it was randomly exposed to the glass tank of the drug solution treatment group, and the drug solution was replaced with the same concentration of drug for 24 hours. In order to keep the concentration of the drug solution as constant as possible, the poison exposure was carried out; respectively, 4 μg / L triazophos, 20 μg / L triazophos, 14 μg / L fipronil and 71 μg / L fipronil were used for poisoning 12 days, and the solvent-treated larvae were used as a parallel control, and samples were taken at the end of the exposure time, and 5 fish were taken each time as a treate...
Embodiment 2
[0074] A method for measuring the fluidity of mitochondrial membranes in the liver of wheat ear carp in vivo, characterized in that it comprises the following steps:
[0075] S1. Fish exposure experiment
[0076] The water body poisoning method was adopted for the exposure experiment of the wheat ear fish body: the wheat ear fish was domesticated for 10 days indoors, and the mortality rate was stabilized below 5%, and it was randomly exposed to the glass tank of the drug solution treatment group, and the drug solution was replaced with the same concentration of drug for 12 hours. In order to keep the concentration of the drug solution as constant as possible, exposure was carried out; 8 μg / L triazophos, 30 μg / L triazophos, 10 μg / L fipronil and 50 μg / L fipronil were used for poisoning respectively. 12 days, and the solvent-treated larvae were used as a parallel control, and samples were taken at the end of the exposure time, and 5 fish were taken each time as a treated sample. ...
Embodiment 3
[0097] A method for measuring the fluidity of mitochondrial membranes in the liver of wheat ear carp in vivo, characterized in that it comprises the following steps:
[0098] S1. Fish exposure experiment
[0099] The water body poisoning method was adopted in the exposure experiment of the wheat ear fish body: the wheat ear fish was domesticated for 16 days indoors, and the mortality rate was stabilized below 5%, and it was randomly exposed to the glass tank of the drug solution treatment group, and the drug solution was replaced with the same concentration of drug for 20 hours. In order to keep the concentration of the drug solution as constant as possible, the poison exposure was carried out; respectively, 4 μg / L triazophos, 20 μg / L triazophos, 14 μg / L fipronil and 71 μg / L fipronil were used for poisoning 12 days, and the solvent-treated larvae were used as a parallel control, and samples were taken at the end of the exposure time, and 5 fish were taken each time as a treate...
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