Method for preparing manno-oligosaccharide from palm kernel meal by microwave-assisted low alkali

A technology of mannooligosaccharides and palm kernel meal, which is applied in the field of functional oligosaccharides, can solve the problems of less than 1% extraction rate of mannooligosaccharides, high cost, and long industrialization distance, etc., so as to be beneficial to environmental protection and reduce The effect of dosage and high economic value

Inactive Publication Date: 2017-12-12
SHANGHAI LAMP BIOLOGICAL SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, our research shows that the extraction rate of mannan oligosaccharides in palm kernel meal treated with mannanase is less than 1%, which is not enough for the industrial production of manno oligosaccharides by this method.
At present, there are only reports on enzymatic degradation of palm kernel meal to extract manno-oligosaccharides. The extraction rate of manno-oligosaccharides is less than 1%, the cost is high, and the industrialization distance is far away.

Method used

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  • Method for preparing manno-oligosaccharide from palm kernel meal by microwave-assisted low alkali
  • Method for preparing manno-oligosaccharide from palm kernel meal by microwave-assisted low alkali
  • Method for preparing manno-oligosaccharide from palm kernel meal by microwave-assisted low alkali

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Experimental program
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Effect test

Embodiment 1

[0027] A microwave-assisted low-alkali method for preparing mannooligosaccharides from palm kernel dregs:

[0028] Step 1): palm kernel meal pretreatment: crushing the palm kernel meal to a fineness of 50-60, 80-100, 200, 400 meshes and sieving respectively;

[0029] Step 2): Mix the pulverized palm kernel meal according to palm kernel meal: water=1:5, add water and stir evenly, then microwave-assisted extraction, the frequency of microwave is 2450mHz, adjust the pH value to 10 with lye, and process for more than 10 minutes ;

[0030] Step 3): After hydraulically filtering or centrifuging the mixture obtained in step 2), take the supernatant, adjust its pH value to 7, enzymatically hydrolyze with mannanase for 7-12 hours, and then concentrate in vacuo;

[0031] Step 4): The concentrated solution obtained in step 3) is centrifuged after alcohol precipitation, and the obtained precipitate is freeze-dried to obtain the crude product of mannooligosaccharides. The alcohol used for...

Embodiment 2

[0036] A microwave-assisted low-alkali method for preparing mannooligosaccharides from palm kernel dregs:

[0037] Step 1): palm kernel meal pretreatment: crushing the palm kernel meal to a fineness of 200 mesh and sieving;

[0038] Step 2): Mix the pulverized palm kernel meal according to palm kernel meal: water = 1:5, add water and stir evenly, then microwave-assisted extraction, the frequency of microwave is 2450mHz, and use saturated sodium hydroxide solution to adjust the pH value to 8, 9, 10, 11, 12, for more than 10 minutes;

[0039] Step 3): After hydraulically filtering or centrifuging the mixture obtained in step 2), take the supernatant, adjust its pH value to 7, use mannanase to enzymatically hydrolyze for 10 hours, and then concentrate in vacuo;

[0040] Step 4): The concentrated solution obtained in step 3) is subjected to alcohol precipitation and centrifuged, and the obtained precipitate is freeze-dried to obtain the crude mannooligosaccharide. The alcohol use...

Embodiment 3

[0043] A microwave-assisted low-alkali method for preparing mannooligosaccharides from palm kernel dregs:

[0044] Step 1): palm kernel meal pretreatment: crushing the palm kernel meal to a fineness of 200 mesh and sieving;

[0045] Step 2): After the pulverized palm kernel meal is mixed with water according to palm kernel meal: water=1:5, microwave-assisted extraction, the frequency of microwave is 2500mHz, and the pH value is adjusted to 10 respectively by using saturated sodium hydroxide solution. Treat 6min, 8min, 10min, 12min, 14min respectively;

[0046]Step 3): After hydraulically filtering or centrifuging the mixture obtained in step 2), take the supernatant, adjust its pH value to 7, enzymatically hydrolyze with mannanase for 8 hours, and then concentrate in vacuo;

[0047] Step 4): The concentrated solution obtained in step 3) is subjected to alcohol precipitation and centrifuged, and the obtained precipitate is freeze-dried to obtain the crude mannooligosaccharide....

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Abstract

The invention discloses a method for preparing manno-oligosaccharide from palm kernel meal by microwave-assisted low alkali. The method is characterized by comprising the following steps: carrying out microwave-assisted extraction on the crushed palm kernel meal; carrying out filter pressing or centrifuging on a mixed solution, then taking supernatant, and adjusting a pH value of the supernate to be 7; carrying out enzymolysis on the supernatant with mannase for 7 to 12 hours and then carrying out vacuum concentration; carrying out alcohol precipitation on a concentrated solution and then centrifuging to obtain a precipitate, and freeze-drying the obtained precipitate, thus obtaining a manno-oligosaccharide crude product. According to the method disclosed by the invention, the palm kernel meal is selected as a raw material and is subjected to crushing, sieving and microwave-assisted alkali treatment, so that the use amount of the alkali is reduced and the extraction effect of the alkali is improved; after solid-liquid separation is ended, a palm kernel meal pretreatment product with a good enzymolysis effect is obtained; in addition, the hindering effect of lignin on degradation of mannan in the palm kernel meal is reduced, and thereby the efficiency of converting the mannan in the palm kernel meal into the manno-oligosaccharide is effectively improved; besides, the dosage of a chemical reagent is reduced, environmental protection is facilitated and the production cost is reduced, thereby obtaining higher economic value.

Description

technical field [0001] The invention relates to a method for preparing mannan oligosaccharides from palm kernel dregs with low alkalinity assisted by microwaves, and belongs to the technical field of functional oligosaccharides. Background technique [0002] Palm kernel meal is a by-product of palm kernel dehulling and oil extraction. The mannan content in palm kernel meal is very high, and it is the best raw material for the preparation of mannan oligosaccharides. The study found that 78% of the non-starch polysaccharides of palm kernel meal are linear mannan (containing a small amount of galactose), 12% of cellulose, 3% of glucuronoxylan and 3% of arabinoxylan Sugar (Dusterhoft et al., 1992). Daud and Jarvis (1992) also reported that palm kernel meal also contains a small amount of galactomannan, which may be a small part of soluble non-cellulosic polysaccharides. Mannan in palm kernel meal is a polymer of mannose with β-branches, which constitutes 39% of the total dry m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/12
CPCC12P19/04C12P19/12
Inventor 刘国庆钱晓勇赵肖孟德连王祝平
Owner SHANGHAI LAMP BIOLOGICAL SCI & TECH
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