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Method for quickly detecting polyaromatic hydrocarbons in seawater or marine sediments by utilizing gene expression change

A technology for marine sediments and polycyclic aromatic hydrocarbons, which is applied in the field of polycyclic aromatic hydrocarbon pollution, can solve problems such as low sensitivity and long cycle, and achieve the effects of high reliability, stable and reliable quality, and easy large-scale promotion and application.

Active Publication Date: 2017-12-12
GUANGDONG LAB ANIMALS MONITORING INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to: overcome the problems of long period and low sensitivity in the detection of existing marine polycyclic aromatic hydrocarbons, and provide a rapid detection method for polycyclic aromatic hydrocarbons in seawater or marine sediments, so as to realize the rapid detection and detection of polycyclic aromatic hydrocarbons. Sensitive and accurate detection

Method used

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  • Method for quickly detecting polyaromatic hydrocarbons in seawater or marine sediments by utilizing gene expression change
  • Method for quickly detecting polyaromatic hydrocarbons in seawater or marine sediments by utilizing gene expression change
  • Method for quickly detecting polyaromatic hydrocarbons in seawater or marine sediments by utilizing gene expression change

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Experimental organisms

[0020] A 120-day-old adult mullet goby was used as the experimental organism. The experimental fish were cultured in a recirculating aquaculture system with a water temperature of 26°C and a salinity of 20°C. Artemia nauplii and compound feed were fed once a day, and half of the water was changed every week. 24 hours before the start of the experiment, the experimental fish were selected for temporary raising in still water. During the temporary raising, the temperature was controlled at 26±0.5°C, the salinity was 20±1, and the dissolved oxygen was above 60% saturation.

[0021] 2. Sample preparation

[0022] The test water is uncontaminated natural seawater, and the salinity is diluted to 20 with aerated tap water. According to Table 1, benzo(a)pyrene with a purity of 99.9% was used to prepare a control group and 3 concentration groups, and each group had 3 repetitions.

[0023] 3. Toxicity test

[0024] The test container is a 2L glass ...

Embodiment 2

[0051] 1. Experimental organisms

[0052]A 90-day-old adult mullet goby was used as the experimental organism. The experimental fish were cultured in a recirculating aquaculture system with a water temperature of 26°C and a salinity of 30°C. Artemia nauplii and compound feed were fed once a day, and 1 / 2 of the water was changed every week. 24 hours before the start of the experiment, the experimental fish were selected and raised in still water temporarily. During the temporary raising period, the temperature was controlled at 26±0.5°C, the salinity was 30±1, and the dissolved oxygen was above 60% saturation.

[0053] 2. Sample preparation

[0054] The test sample was seawater known to be polluted by PAHs in the South China Sea, and the control group was natural seawater without pollution. Prepare 1 control group and 1 concentration group, and set 3 repetitions for each group.

[0055] 3. Toxicity test

[0056] The test container is a 2L glass beaker, the test solution is ...

Embodiment 3

[0081] 1. Experimental organisms

[0082] A 90-day-old adult mullet goby was used as the experimental organism. The experimental fish were cultured in a recirculating aquaculture system with a water temperature of 26°C and a salinity of 30°C. Artemia nauplii and compound feed were fed once a day, and 1 / 2 of the water was changed every week. 24 hours before the start of the experiment, the experimental fish were selected and raised in still water temporarily. During the temporary raising period, the temperature was controlled at 26±0.5°C, the salinity was 30±1, and the dissolved oxygen was above 60% saturation.

[0083] 2. Sample preparation

[0084] The samples were sediments known to be polluted by PAHs in a port in the South China Sea, and the dilution water was unpolluted natural seawater with a salinity of 30. Take the sediment and dilution water, mix them in a volume ratio of 1:4, stir at 2000r / min for 30 minutes, let stand for 1 hour, and take the supernatant for toxic...

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Abstract

The invention discloses a method for quickly detecting polyaromatic hydrocarbons in seawater or marine sediments by utilizing gene expression change. The method disclosed by the invention is used for detecting the polyaromatic hydrocarbons in seawater or the marine sediments by taking mugilogobius chulae as an experimental animal, and a mugilogobius chulae cytochrome P4502K3 gene, a multidrug-resistant gene 1 or a phosphatide phosphatase gene as biological markers. The method disclosed by the invention is used for quickly detecting the polyaromatic hydrocarbons by using a standardized organism as the experimental animal for the first time and through screening the three screened novel biological markers, has the advantages of being high in credibility, good in repeatability, free from background pollution risk, short in detection period, high in sensitivity, low in cost and the like, plays a great role in promoting a current marine polyaromatic hydrocarbon detection technology and has good popularization and application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological detection of marine polycyclic aromatic hydrocarbons, more specifically, the invention relates to a rapid detection method using the mullet goby and its cytochrome P4502K3 gene, multi-drug resistance gene 1 or phosphatidic acid phosphatase gene Methods of PAH pollution in seawater or marine sediments. Background technique [0002] As a kind of persistent organic pollutants, polycyclic aromatic hydrocarbons (PAHs) mainly come from incomplete combustion of fossil fuels. They are highly carcinogenic, teratogenic and mutagenic, and many countries have listed them as key monitoring items. After years of sustained and rapid economic development in China, coastal areas have suffered from varying degrees of PAHs pollution. In some areas of the Liaodong Peninsula in the Bohai Sea, the concentrations of PAHs in seawater and sediments have reached 1717.87mg / L and 1606.89ng / L respectively. The concentratio...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 黄韧蔡磊李建军余露军魏远征苗宗余陈梅丽
Owner GUANGDONG LAB ANIMALS MONITORING INST
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