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Primer combination and application and detection kit for detecting animal-derived components

A detection kit and a technology for detecting animals, which are applied in the field of biological and biological inspection and quarantine, can solve problems such as few reports, and achieve the effects of strong pertinence, favorable for application and promotion, and accurate and reliable detection results.

Active Publication Date: 2021-01-12
HEILONGJIANG ZBD PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are few reports on the genetic level detection of animal-derived components in osteopeptide, and targeted detection methods are needed

Method used

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  • Primer combination and application and detection kit for detecting animal-derived components
  • Primer combination and application and detection kit for detecting animal-derived components
  • Primer combination and application and detection kit for detecting animal-derived components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] This embodiment provides a primer combination for detecting animal-derived components, which includes the first pair of primers for detecting porcine-derived components; the base sequences of the first primer pair are shown in SEQ ID No. 1-2.

Embodiment 2

[0051]This embodiment provides a primer combination for detecting animal-derived components, which includes the first primer pair for detecting porcine-derived components, the second primer pair for detecting horse-derived components, the third primer pair for detecting bovine-derived components, and the third primer pair for detecting sheep-derived components. At least one of the fourth primer pair of the source component; the base sequences of the first-fourth primer pair are respectively shown in SEQ ID No.1-8.

Embodiment 3

[0053] This embodiment provides a detection kit for detecting animal-derived components, which includes the primer combination for detecting animal-derived components provided in Example 1.

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PUM

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Abstract

The invention provides a primer composition for detecting animal source components and application and a detection kit, and belongs to the field of biological inspection and quarantine. The primer composition for detecting the animal source components, which is provided by the invention, is good in pertinency, high in specificity and relatively good in sensitivity; the primer composition for detecting the animal source components is used for detecting animal components in injection ossotide, detection results can be relatively accurate and reliable, and the quality of a finished product can be effectively controlled; the primer composition is applied to preparation of detection kits for detecting animal source components, animal source components of injection ossotide raw materials can be relatively conveniently and rapidly detected, application and popularization of the kit are facilitated, and relatively high reagent application values and popularization values can be made.

Description

technical field [0001] The invention relates to the field of biological and biological inspection and quarantine, in particular to a combination of primers for detecting animal-derived components, its application, and a detection kit. Background technique [0002] Osteopeptide is widely used clinically and has good curative effect. Studies have shown that osteopeptide contains a variety of active peptides that promote bone metabolism and inorganic elements such as calcium, phosphorus, and iron, which can regulate bone metabolism, stimulate osteoblast proliferation, promote new bone formation, and regulate calcium and phosphorus metabolism; Significantly enhance bone calcium metabolism and deposition, prevent osteoporosis; have obvious anti-inflammatory and analgesic effects, and play an important regulatory role in the repair and formation of bone tissue. Including bone morphogenetic protein (BMP), transforming growth factor-β (TGF-β), fibroblast growth factor (FGF), insuli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888
Inventor 方同华许照芹项彦华席桂才代喜国
Owner HEILONGJIANG ZBD PHARMA
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