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Actinomycetes-containing biocontrol preparation for preventing and controlling diseases of pseudo-ginsengs and application method

By using special actinomycete biocontrol preparations in Panax notoginseng cultivation, the problems of disease prevalence and pesticide residues in Panax notoginseng cultivation have been solved, yield and quality have been improved, and efficient and convenient disease control has been achieved.

Active Publication Date: 2017-12-15
JILIN PROVINCIAL ACADEMY OF FORESTRY SCIENCES JILIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Harmful germs reproduce in large numbers, causing epidemics and outbreaks of crop diseases, resulting in severe production reductions and even crop failures
At present, in order to control the prevalence of diseases of Panax notoginseng, a large amount of pesticides are usually used in the planting process, which may easily cause pesticide residues, reduce the quality of Panax notoginseng, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Prepare 1L culture medium, which includes the following components: 30g / L soybean powder, 8g / L starch, 3g / L sucrose, 2g / L yeast powder, 2g / L sodium chloride, 1g / L hydrogen phosphate Dipotassium, 0.2g / L MgSO 4 ·7H 2 O, 0.01g / L zinc sulfate, 0.01g / L FeSO 4 ·7H 2 O, the pH is 7.2; after the preparation is completed, sterilize at 121°C for 30 minutes;

[0031] 2) inoculate actinomycetes according to mass percentage 7% (the quality of actinomycetes accounts for 7% of the quality of culture medium, the implication of following embodiment is also the same as this embodiment), culture temperature is 30 ℃, culture time is 24h, The speed is 150rpm;

[0032] 3) Lower the temperature to 24°C at a rate of 1°C / h, and then culture for 12 hours;

[0033] 4) prepare the brown sugar solution of 500g / L, filter and sterilize;

[0034] 5) Add 60mL of the above-mentioned filter-sterilized brown sugar solution to the culture medium at a temperature of 24°C and incubate for 48h;

[0...

Embodiment 2

[0037] 1) Prepare 1L culture medium, which includes the following components: 30g / L soybean powder, 8g / L starch, 3g / L sucrose, 2g / L yeast powder, 2g / L sodium chloride, 1g / L hydrogen phosphate Dipotassium, 0.2g / L MgSO 4 ·7H 2 O, 0.01g / L zinc sulfate, 0.01g / L FeSO 4 ·7H 2 O, the pH is 7.2; after the preparation is completed, sterilize at 121°C for 30 minutes;

[0038] 2) Actinomycetes were inoculated according to 8% by mass, the culture temperature was 30°C, the culture time was 24h, and the rotation speed was 150rpm;

[0039] 3) Lower the temperature to 24°C at a rate of 1°C / h, and then culture for 12 hours;

[0040] 4) prepare the brown sugar solution of 500g / L, filter and sterilize;

[0041] 5) Add 60mL of the above-mentioned filter-sterilized brown sugar solution to the culture medium at a temperature of 24°C and incubate for 48h;

[0042] 6) Collect, pack, and prepare biocontrol preparations containing actinomycetes, the number of viable bacteria in the biocontrol pre...

Embodiment 3

[0044] 1) Prepare 1L culture medium, which includes the following components: 30g / L soybean powder, 8g / L starch, 3g / L sucrose, 2g / L yeast powder, 2g / L sodium chloride, 1g / L hydrogen phosphate Dipotassium, 0.2g / L MgSO 4 ·7H 2 O, 0.01g / L zinc sulfate, 0.01g / L FeSO 4 ·7H 2 O, the pH is 7.2; after the preparation is completed, sterilize at 121°C for 30 minutes;

[0045]2) inoculate actinomycetes according to the mass percentage of 10%, the culture temperature is 30°C, the culture time is 24h, and the rotation speed is 150rpm;

[0046] 3) Lower the temperature to 24°C at a rate of 1°C / h, and then culture for 12 hours;

[0047] 4) prepare the brown sugar solution of 500g / L, filter and sterilize;

[0048] 5) Add 60mL of the above-mentioned filter-sterilized brown sugar solution to the culture medium at a temperature of 24°C and incubate for 48h;

[0049] 6) Collect, pack, and prepare biocontrol preparations containing actinomycetes, the number of viable bacteria in the biocontr...

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Abstract

The invention relates to an actinomycetes-containing biocontrol preparation for preventing and controlling diseases of pseudo-ginseng and an application method. The actinomycetes-containing biocontrol preparation is prepared through the following steps: preparing 1L of a culture medium; after preparing the culture medium, sterilizing at 121 DEG C for 30min; inoculating actinomycetes according to the mass ratio of 7 percent to 10 percent, wherein the culture temperature is 30 DEG C, the culture time is 24h and the rotary speed is 150rpm; cooling to 24 DEG C according to the rate of 1 DEG C / h, and then culturing for 12h; preparing a 500g / L brown sugar solution, filtering and sterilizing; adding 20mL of the filtered and sterilized brown sugar solution into the culture medium and culturing at the temperature of 24 DEG C for 48h; collecting and packaging to obtain the actinomycetes-containing biocontrol preparation. The actinomycetes-containing biocontrol preparation prepared by the above method can be used for effectively controlling the diseases of plants and remarkably improving the yield and quality of the plants, and is especially suitable for the pseudo-ginseng.

Description

[0001] This application is a divisional application with application number 201510607650.7, application date of September 23, 2015, and title of the invention "a preparation method of a biocontrol agent containing actinomycetes". technical field [0002] The invention relates to the field of preparation of biocontrol preparations, in particular to a biocontrol preparation containing actinomycetes for the prevention and control of Panax notoginseng disease and its use method. Background technique [0003] Actinomycetes are a group of microorganisms that grow in soil, on the surface and inside of plants, as well as in fresh water or oceans. They are the earliest microorganisms studied and widely used in agricultural production. Most of the antibiotic-producing bacteria found so far are actinomycetes, and more than 80% of the antibiotics are from the genus Streptomyces. Streptomyces mainly exists in the soil. After the spores germinate, they first form basal hyphae, and then di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P1/00A01P3/00A01P21/00C12N1/20
CPCA01N63/00C12N1/20
Owner JILIN PROVINCIAL ACADEMY OF FORESTRY SCIENCES JILIN