Efficient pre-treatment device for culturing human umbilical cord mesenchymal stem cells and related application thereof

A pretreatment device and stem cell technology, applied in the direction of biomass pretreatment, biomass post-treatment, bioreactor/fermenter for specific purposes, etc. cumbersome and other problems, to achieve the effect of promoting the use, low cost and stable technology

Active Publication Date: 2017-12-15
上海莱威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the human umbilical cord tissue is pretreated, the human umbilical cord tissue needs to be pretreated in different processing equipment or containers, and the human umbilical cord tissue is easily polluted by microorganisms such as bacteria and viruses in the air in this large space tran

Method used

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  • Efficient pre-treatment device for culturing human umbilical cord mesenchymal stem cells and related application thereof
  • Efficient pre-treatment device for culturing human umbilical cord mesenchymal stem cells and related application thereof
  • Efficient pre-treatment device for culturing human umbilical cord mesenchymal stem cells and related application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0062] Example 1 figure 1 As shown, this embodiment provides a high-efficiency pretreatment device for human umbilical cord mesenchymal stem cells, which specifically includes: a reversible upper cover 100, the upper cover 100 is provided with a knife groove 101, the knife groove 101 is composed of at least two groups The grid-like sub-knife grooves of different depths are composed of each other; the housing 200 is hingedly connected to the upper cover 100; the cutting assembly 300 is located at the opening of the housing 200, and the cutting assembly 300 is arranged in cooperation with the upper cover 100 for washing The rear umbilical cord segment is cut into small pieces of tissue of the same size; the cutting assembly 300 is composed of a grid-shaped blade 301 and an elastic sealing ring 302, and the grid-shaped blade 301 is composed of at least two sets of sub-blades arranged staggered up and down. And the grid-shaped blade 301 is arranged corresponding to the knife groove ...

Example Embodiment

[0076] Embodiment 2 This embodiment provides a method for pretreatment of human umbilical cord mesenchymal stem cells using the high-efficiency pretreatment device of Embodiment 1, including the following steps:

[0077] Step 1) Obtain human umbilical cord tissue

[0078] Obtain the human umbilical cord tissue of the newborn, try to use the umbilical cord of the cesarean section to avoid contamination; put the umbilical cord section in ice saline and keep it at a low temperature of about 4 degrees and quickly bring it back to the cell laboratory. In the safety cabinet, fully wash the umbilical cord with ice-cold saline to remove blood and impurities. After removing the umbilical artery and umbilical vein, fold the umbilical cord into 5-8 layers of 5cm×5cm tissue blocks. The tissue block is placed on the cutting assembly 300, the cutting specifications of the grid-like blade 301 and the knife groove 101 are selected according to the needs, and the upper cover 100 is pressed down to ...

Example Embodiment

[0083] Example 3 This example provides a method for culturing human umbilical cord mesenchymal stem cells pretreated in Example 2, which includes the following steps:

[0084] Step 1) Medium preparation

[0085] Prepare DMEM / F12 medium containing 20% ​​fetal bovine serum and 1% penicillin streptomycin, and DMEM / F12 medium containing 10% fetal bovine serum and 1% penicillin streptomycin; in order to improve the mesenchyme of human umbilical cord The adherent survival rate of stem cells is cultured in DMEM / F12 medium containing 20% ​​fetal bovine serum and 1% penicillin on the first day of primary culture, passage and recovery; at other times, use 10% fetal Cultivate in DMEM / F12 medium with bovine serum and 1% penicillin;

[0086] Step 2) Two-dimensional training

[0087] Take the relatively pure human umbilical cord mesenchymal stem cells pretreated in Example 2, and resuspend the relatively pure human umbilical cord mesenchymal stem cells in the above medium to prepare a human umbili...

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Abstract

The invention discloses an efficient pre-treatment device for culturing human umbilical cord mesenchymal stem cells and a related application thereof. The efficient pre-treatment device comprises a turnover upper cover, a cutting assembly, a digestion assembly and a cleaning centrifugal part, wherein a cutter groove is formed in the upper cover; the cutting assembly located at an opening of a shell is composed of latticed blades and an elastic seal ring and the latticed blades and the cutter groove are correspondingly arranged; the digestion assembly is located in the middle of the shell; and the cleaning centrifugal part is located on the lower portion of the shell. The invention also provides a cell pre-treatment and culture method based on the efficient pre-treatment device. The method comprises the following six steps: human umbilical cord cutting and separating; enzymic digestion; centrifugal purification; culture medium preparation; two-dimensional culture; and subculture. The cell culture method based on the efficient pre-treatment device disclosed by the invention is stable in technology and good in repeatability, avoids the problems of low cell activity and poor yield, improves the cell activity and yield greatly, simplifies the operating process, and is flexible and convenient to use.

Description

technical field [0001] The invention belongs to the technical field of human cell culture, in particular to a high-efficiency pretreatment device for culturing human umbilical cord mesenchymal stem cells and its corresponding application Background technique [0002] Mesenchymal stem cells are a kind of very characteristic adult stem cells. These cells have the potential to differentiate into a variety of mature cells including: adipocytes, chondrocytes, bone cells, tenocytes and muscle cells. These properties, as well as their developmental plasticity, have drawn considerable attention from the scientific community as they could be exploited in regenerative medicine research. In vitro, mesenchymal stem cells isolated from umbilical cord Wharton's jelly can differentiate into neurons and glial cells after being induced by nerve-directed induction medium, Shh and FGF8. Mesenchymal stem cells can also be differentiated into adipocytes and osteoblasts. In addition, in the my...

Claims

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Application Information

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IPC IPC(8): C12M1/40C12M1/00C12N5/0775
CPCC12M21/18C12M45/02C12N5/0665C12N2509/00
Inventor 王晓冰
Owner 上海莱威生物科技有限公司
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