Sample pretreatment method for determining nanoscale cerium oxide in plasma or serum

Abstract

The invention discloses a sample pre-treatment device for determining nano-grade cerium oxide in blood plasma or blood serum by adopting an inductively coupled plasma mass spectrometer. The sample pre-treatment device comprises the following steps: step 1, uniformly distributing nano-grade cerium oxide particles; step 2, adding a reduction agent into the nano-grade cerium oxide particles; step 3, digesting blood plasma protein or blood serum protein and carrying out reduction treatment on the cerium oxide particles; step 4, carrying out post-treatment. According to the sample pre-treatment device disclosed by the invention, a stock and working solution is prepared by adopting a mixed solution of nitric acid and Triton-X-100 and the nano-grade cerium oxide particles stably exist in the solution; a manner of digesting in a water bath at 80 DEG C for one night is adopted and the blood plasma protein or the blood serum protein is digested to reduce cerium oxide into trivalent cerium ions, so that accurate detection of an inductively coupled plasma mass spectrum can be carried out. According to the sample pre-treatment device disclosed by the invention, a nano-grade cerium oxide particle solution is stable and the protein is completely digested; a method is simple to operate and the testing cost is low; the determination of a large batch of blood plasma samples can be realized in one step.

Description

technical field [0001] The invention relates to a sample processing method, in particular to a sample pretreatment method for measuring nanoscale cerium oxide in plasma or serum by using an inductively coupled plasma mass spectrometer. Background technique [0002] Cerium oxide is a rare earth oxide. Nano-sized cerium oxide has the function of removing free radicals in the human body and preventing the spread of cancer cells. It has great medicinal value in medicine. [0003] In 2013, when Wason MS et al. conducted a study on mice inoculated with human pancreatic cancer cells, they found that nano-cerium oxide particles were beneficial to the scavenging of superoxide free radicals, and that nano-cerium oxide was used in the radiotherapy matrix before pancreatic cancer in mice. Can significantly reduce the cancer cells in the culture medium and tumors and protect normal cells. The results show that nano-cerium oxide may become a novel photosensitizer for the treatment of pan...

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Problems solved by technology

But among them, the microwave digestion method requires a special microwave digestion system, and cannot digest dozens or even hundreds of samples at the same time, so it is not suitable for the digestion of large quantities of blood samples; the main disadvantage of the enzymatic hydrolysis method is the need to control the amount of enzyme added and the time of enzymatic hydrolysis , enzymatic hydrolysis temperature and other related process conditions, the operation is more complicated, and special protease needs to be purchased, resulting in increased test costs

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