Universal microfluidic chip apparatus for simultaneous detection of multiple tumor markers

Abstract

The present invention relates to a universal microfluidic chip apparatus for simultaneous detection of multiple tumor markers, and belongs to the field of analytical testing. According to the present invention, the use of PDMS to produce the substrate of a universal microfluidic chip for combined detection of more than ten kinds of typical tumor markers has a series of problems that the surface of the PDMS is strongly hydrophobic and the surface chemical modification effect is difficult to last. The technical scheme of the present invention is that the substrate material is PDMS with an original ecological surface, the pipeline of the chip is filled with doped micron-scale titanium dioxide particles, the surface super-hydrophilicity of the titanium dioxide particles can be induced by visible light irradiation in advance, and the set of the titanium dioxide particle filler and the super-hydrophilic gap between the particles can compensate and beyond the original strong hydrophobic pipeline, such that the constant and super hydrophilic modification effect is achieved. In addition, the titanium dioxide filler can further be used for preventing the pipeline of the chip from collapsing and deforming, and blocking biological macromolecules from contacting the inner wall of the pipeline.

Description

Technical field [0001] The invention relates to a universal microfluidic chip device for simultaneously detecting multiple tumor markers, belonging to the field of analysis and testing. Background technique [0002] Tumor marker (TM) refers to a class of substances that are produced by tumor cells themselves or by the body’s response to tumor cells during the occurrence and proliferation of tumors, and reflect the existence and growth of tumors, including Proteins, hormones, enzymes (isoenzymes) and oncogene products, etc. Testing the tumor markers in the blood or body fluid of the patient can detect the tumor early in the tumor screening, and observe the efficacy of tumor treatment and judge the prognosis of the patient. Currently commonly used clinical tumor markers are: (1) alpha-fetoprotein (AFP) is a marker for primary liver cancer, testicular cancer, ovarian cancer and other tumors; (2) carcinoembryonic antigen (CEA) is a tumor of the digestive system, Markers for lung ca...

AI Technical Summary

Problems solved by technology

[0008] But it's not that simple

[0009] First, this polydimethylsiloxane material, which is referred to by the acronym PDMS, is itself a strongly hydrophobic material, and channels are built on this material. modification operation, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the channel in the structure occupies most of the inner surface of the liquid flow channel, then the inner surface of the PDMS channel is The strong hydrophobicity is the decisive factor, it will make it very difficult for the polar liquid fine flow similar to the aqueous solution to pass through, and its flow resistance is so large that even the general micropump is difficult to push, of course, if the cover sheet is also If you choose to use the PDMS material, then the problems are basically the same, with minor differences; therefore, in the prior art, it is necessary to modify and modify the inner surface of the channel on the PDMS material; then, this is for the PDMS channel Is the modification operation of the inner surface of the channel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the channel modified by the surface unable to maintain for a long enough time. The maintenance time of the internal surface state is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the surface state of the PDMS channel inner surface after surface modification or surface modification is formed after modification It cannot last for a long time, but it will automatically tend to or change back to the surface state before the surface modification, and return to the original strongly hydrophobic surface state in a short period of time. Then, just imagine, such a slight Can flow control chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

This problem has also existed for many years, and so far, it has not been properly solved

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