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Method for treating benzoic-acid-containing waste water and anaerobic biodegradation method for benzoic acid

An anaerobic biology and treatment method technology, which is applied in the field of anaerobic biodegradation of benzoic acid and the treatment of wastewater containing benzoic acid, can solve the problem of low anaerobic biodegradation rate of benzoic acid, and achieve the effect of increasing the degradation rate

Inactive Publication Date: 2017-12-22
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the natural system, due to the complex environmental factors, the anaerobic biodegradation rate of benzoic acid is very low, so it is of great practical value to seek a method to significantly increase the anaerobic biodegradation rate of benzoic acid in an anaerobic system.

Method used

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  • Method for treating benzoic-acid-containing waste water and anaerobic biodegradation method for benzoic acid
  • Method for treating benzoic-acid-containing waste water and anaerobic biodegradation method for benzoic acid
  • Method for treating benzoic-acid-containing waste water and anaerobic biodegradation method for benzoic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Prepare 500mL of medium concentrate: weigh 5g KH 2 PO 4 ,50gNH 4 Cl, 40gNaCl, 10gMgSO 4 ·7H 2 O,2gCaCl 2 2H 2 O was dissolved in a beaker filled with 500ml of deionized water. After the dissolution was complete, it was transferred to a 500mL blue-necked bottle for later use. Other ingredients are shown in Table 2.

[0049] Configure 1000 times of trace element and vitamin concentrates, as shown in Table 3 and Table 4.

[0050] Prepare 20g / L multi-walled carbon nanotubes: first dissolve 2g of multi-walled carbon nanotubes into a blue-necked bottle filled with 100ml of deionized water, mix well after ultrasonication for 30min, and set aside.

[0051] Configure 1M sodium benzoate stock solution: weigh 14.4g of sodium benzoate and dissolve it in a beaker filled with 100ml of deionized water, then transfer it to a 120ml serum bottle, and use N 2 :CO 2 = After 15 minutes of aeration with the mixed gas of 4:1, package it for later use.

[0052] Take 12 clean serum bot...

Embodiment 2

[0073] Taking activated carbon as an example, the effects of different concentrations of activated carbon on the anaerobic degradation rate of benzoic acid were explored.

[0074] Take 12 clean serum bottles with a volume of 120 mL, add different concentrations of activated carbon, transfer them to an anaerobic glove box, and add 45 mL of medium solution respectively.

[0075] Then, take out 100ml of the mother liquor containing benzoic acid-degrading bacteria, after centrifuging at 8000g, remove the supernatant, put into the glove box and dissolve the mud at the bottom with 100ml of new medium, pour it into a 250mL beaker, and Mix evenly under the agitator, then transfer 4.5mL to 12 vials with a 5mL pipette gun, respectively add to 120ml serum bottles (to ensure the same inoculum volume), and package.

[0076] Add 0.5 mL of benzoic acid stock solution to each vial, then wash with N 2 :CO 2 = 4:1 mixed gas replaces the headspace gas, and then cultures at 37°C in a dark and v...

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Abstract

The invention provides an anaerobic biodegradation method for benzoic acid. The anaerobic biodegradation method for benzoic acid comprises the step of carrying out anaerobic biodegradation on the benzoic acid by using benzoic acid degrading bacteria in the presence of a conductive carbon material. The conductive carbon material is added in a benzoic acid anaerobic degradation process, the benzoic acid anaerobic degradation speed can be increased, the method can be used for treating the benzoic-acid-containing waste water, and can also be used for treating benzoic-acid-containing solid wastes, the treatment speed is increased, and the treatment ability is improved. Experimental results show that by the conductive carbon material, anaerobic degradation of the benzoic acid can be promoted, and along with increasing of the concentration of the conductive carbon material, the biodegradation speed of the benzoic acid is increased.

Description

technical field [0001] The invention belongs to the technical field of organic wastewater treatment, and in particular relates to a treatment method for benzoic acid-containing wastewater and a benzoic acid anaerobic biodegradation method. Background technique [0002] Aromatic compounds are one of the most abundant compounds on earth. They exist in nature in various forms such as phenolic resins, lignin monomers in plants, and aromatic hydrocarbons. With the rapid development of industry and agriculture, aromatic compounds are widely used in plastics, food, dyes, leather, synthetic rubber and organic synthesis industries, etc., resulting in more and more aromatic compounds entering the environment, which are harmful to animals, plants and humans. Survival constitutes a great hazard, especially organic pollutants such as teratogenic, carcinogenic, and mutagenic small molecules such as phenol and aniline. [0003] On the other hand, when using chemical methods to treat high-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F3/34C12N1/36C02F101/34
CPCC02F3/28C02F3/34C02F2101/34C12N1/36
Inventor 曾建雄王贤斌
Owner UNIV OF SCI & TECH OF CHINA
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