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Rice kosakonia oryzae HN05 and application thereof

A Saxobacteria sp., M2021956HN05 technology, applied in rice Kossackia sp. HN05 and its application fields, can solve the problems such as no Kosakoniasp. literature reports, achieve good application prospects, promote anaerobic degradation, and broad spectrum of electron utilization Effect

Active Publication Date: 2022-01-11
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are currently 9 species of Kosakonia sp., and it is reported that except for Kosakonia quasisacchari and Kosakonia cowanii, which are isolated from human body, the other 7 species of Kosakonia sp. are all isolated from the rhizosphere of plants. They have the ability to fix nitrogen and are rice , corn, wheat, groundnut and other plants' dominant nitrogen-fixing bacteria, there is no literature report on the ability of Kosakonia sp. to reduce anthraquinone and degrade diquat

Method used

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  • Rice kosakonia oryzae HN05 and application thereof
  • Rice kosakonia oryzae HN05 and application thereof
  • Rice kosakonia oryzae HN05 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The enrichment and separation of embodiment 1-rice cossackia HN05

[0025] 1) Take 5g of sediment samples in 100mL anaerobic liquid medium under aseptic operation conditions, 0.5mmol / LAQDS (anthraquinone-2,6-sodium disulfonate, electron acceptor), 2.5g NaHCO 3 , 0.25g NH 4 Cl, 0.68gNaH 2 PO 4 2H 2 O, 0.1g KCl, 10.0mL vitamin stock solution, 10.0mL trace element stock solution.

[0026] Among them, the vitamin solution contains 5.0mg of folic acid, 0.2mg of vitamin B6, 6.5mg of vitamin B2, 3.0mg of vitamin B1, 10mg of nicotinamide, 1mg of calcium pantothenate, 0.2mg of vitamin B12, and 2.0mg of vitamin H per liter of deionized water; The solution was 1.6 g EDTA, 3.0 g MgSO per liter of deionized water 4 ·7H 2 O, 0.5g MnSO 4 ·H 2 O, 1.0gNaCl, 0.1gCoCl 2 ·6H 2 O, 0.1g CaCl 2 , 0.01g CuSO 4 ·5H 2 O, 0.01g AlK(SO 4 ) 2 12H 2 O, 0.01gH 3 BO 3 , 0.025g Na 2 MoO 4 2H 2 O. Fill the above soil-medium system (N 2 / CO 2 =80 / 20) mixed gas for 30 minutes to d...

Embodiment 2

[0030] Morphological, physiological, biochemical and molecular biological characteristics of embodiment 2-rice Cossackia HN05

[0031] 1) Morphological characteristics of bacteria

[0032] Observed under an optical microscope, the strain is Gram-negative bacteria, straight rod-shaped, single or in pairs. Under the transmission electron microscope, observe the perinatal flagella of the strain (such as figure 1 shown), sporty.

[0033] After aerobic culture at 30°C for 24 hours on the NA agar solid medium plate with a pH of 7.2, the colony is round, with a moist and smooth surface, a convex middle, translucent, and neat edges, and the diameter of the colony is 1 to 3 mm.

[0034] 2) Physiological and biochemical characteristics

[0035] The strain is fermentative and facultative anaerobic; the growth temperature range is 25-37°C (optimum 30°C), the pH range is 4-9 (optimum pH=6), and the NaCl% range is 0-5 (optimum 0.5%); Other physiological and biochemical characteristics a...

Embodiment 3

[0042] Example 3 - Cossackia oryzae HN05 uses different electron donors to reduce AQDS

[0043] The present invention uses AQDS as an electron acceptor to investigate the electron utilization spectrum of Cossackia oryzae HN05.

[0044] The composition of the reaction system: bacterial suspension, inorganic salts, vitamins, trace elements, the composition and content of each material are the same as the above-mentioned enrichment and separation medium; the electron acceptor is 0.5mmol / LAQDS; the electron donor is selected from acetic acid, glycerol, sucrose, or lactic acid, the added concentration is 5mmol / L; 1mL bacterial suspension, inoculate the strain HN05 in NA liquid medium, culture aerobically at 30℃ for 18h, collect the bacterial cells by centrifugation at 8000r / min, and wash the bacterial cells with inorganic salt medium 2 times, and finally suspend the bacteria in fresh inorganic salt medium to make a bacteria suspension.

[0045] Set up three replicates. The AQDS r...

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Abstract

The invention relates to the technical field of environmental microorganisms, and particularly relates to rice kosakonia oryzae HN05 and application thereof. The classification name of the strain is Kosakonia oryzae HN05, and the Kosakonia oryzae HN05 can oxidize and degrade diquat under the anaerobic condition, has the anthraquinone compound reduction activity and is wide in electron utilization spectrum; and meanwhile, the strain HN05 can take a synergistic effect with AQDS, can remarkably promote anaerobic degradation of the diquat, is applied to diquat polluted water treatment and soil remediation, and has a good application prospect in the fields of pesticide pollution treatment and soil remediation.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a strain of Cossackia oryzae HN05 and its application. Background technique [0002] Diquat (1,1'-ethylene-2,2'-bipyridyl dibromide, diquat), is a non-selective contact bipyridyl herbicide, widely used in no-tillage and rapid crop rotation , Live rice and other agricultural and forestry production. Diquat has persistence and environmental health toxicity: Interfering with the oxidative phosphorylation reaction of zebrafish embryos, causing impaired growth of rainbow trout embryos, chronic death of Northwestern salamanders, and resulting in imbalances in aquatic ecosystems; Produces strong toxicity, long-term exposure to diquat can cause reproductive toxicity to female mice, and farmers have an increased risk of Parkinson's disease; acute poisoning of diquat can cause 2,128 diseases, and there is no special effect after bioaccumulation or acute poisoning Ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/01A62D101/04A62D101/26C02F101/30C02F101/36C02F101/38
CPCC02F3/34B09C1/10A62D3/02C02F2101/306C02F2101/36C02F2101/38A62D2101/04A62D2101/26
Inventor 武春媛阴文芳吴东明
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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