Schwann cell extraction, purification and culture method

Abstract

The invention discloses Schwann cell extraction, purification and culture method which comprises the following steps: cutting off brachial plexus and ischiadic nerve, further removing spinal cord, and extracting peripheral nerve from different foramen intervertebrales; digesting the peripheral nerve so as to obtain a single-cell suspension; and performing amplification culture, thereby obtaining Schwann cells. By adopting the method disclosed by the invention, the nerve extracted from the foramen intervertebrales is free of epineuria and is low in fibroblast residue. According to the method, the peripheral nerve (including the brachial plexus, the intercostal nerve and the ischiadic nerve) is extracted from the foramen intervertebrales, a nerve amount which is 6-8 times of that of a conventional method is obtained, the residue of fibroblast is remarkably reduced, and thus culture and amplification of the Schwann cells are facilitated.

Description

technical field [0001] The invention relates to a method for extracting, purifying and culturing Schwann cells. Background technique [0002] Primary cultured Schwann cells are not only the basis for studying Schwann cell function and surrounding myelin biology, but also important seed cells for neural tissue engineering. Glial cells in the peripheral nervous system are called Schwann cells, which are distributed along the processes of neurons. Schwann cells wrap around nerve fibers called myelinated nerve fibers. The morphology and function of Schwann cells of myelinated and unmyelinated nerve fibers are different. The outer surface of Schwann cells has a basement membrane, which can secrete neurotrophic factors and promote the survival of damaged neurons and the growth of axons. Regeneration, involved in the formation of nerve fibers in the peripheral nervous system. Immature Schwann cells can be stimulated by the microenvironment to initiate differentiation again. Spi...

AI Technical Summary

Problems solved by technology

However, in the prior art, the isolation, purification and culture methods of Schwann cells from mice and rats all have the problems of low nerve acquisition and large residual fibroblasts, which is ultimately unfavorable for the primary culture and expansion of Schwann cells. increase, the number of Schwann cells obtained is small and the activity is poor

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