Anti-oxidation, anti-wrinkle and moisturizing improvement functional cosmetic composition containing rice water fermentation culture and preparation method thereof
A cosmetic composition, fermentation culture technology, applied in the anti-wrinkle and moisturizing and improving functional cosmetic composition, anti-oxidation field, can solve the problems such as unpublished anti-oxidation, anti-wrinkle, etc., achieve excellent moisturizing improvement, excellent anti-wrinkle effect
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Embodiment 1
[0026] Embodiment 1: the preparation of rice washing water preparation of the present invention
[0027] 10-40% by weight of rice by total weight is soaked in water, stirred at 100-150 rpm for 1-3 hours at 25°C, and autoclaved at 121°C for 20 minutes to the resulting supernatant, Thus, rice washing water with a hydrogen ion concentration of pH 6 to 8 and a solid content of 0.1 to 3.0 is obtained.
Embodiment 2
[0028] Embodiment 2: Utilize the preparation of the yeast strain fermentation culture of rice washing water preparation
[0029] In order to prepare a fermentation culture using the rice washing water preparation obtained in Example 1 of the present invention, the preparation sterilized in a 2 to 5 L fermenter was inoculated with 2 to 5% of alkali-tolerant Saccharomyces or yeast It belongs to a yeast strain and is stirred at 100-300 rpm, most preferably at 200 rpm for 1-4 days at 25-40° C., most preferably at 30° C., thereby carrying out liquid fermentation. After the cultivation of the yeast strain is completed, pressurize at 90-121° C. for 5-20 minutes, and then pass through a filtration process to obtain the rice-washing water fermentation culture of the present invention.
[0030] From figure 1 and figure 2 It can be seen from the results that the rice washing water fermentation culture of the present invention can improve the color and precipitation problems that are n...
experiment example 1
[0031] Experimental example 1: Antioxidant activity according to DPPH scavenging ability
[0032] For the antioxidant activity of the rice washing water fermentation culture obtained in Example 2 of the present invention, 1,1-diphenyl-2-trinitrophenylhydrazine free radical (1,1-diphenyl-2picrylhydrazyl radical ) (DPPH) to measure the antioxidant activity. DPPH exists in the form of a free radical with a stabilized nitrogen center in the compound, and the antioxidant activity can be measured according to the degree of scavenging ability of the stabilized free radical.
[0033] In order to evaluate these, the rice-washing water fermentation culture obtained in Example 2 of the present invention was used as a test compound, mixed with 100 μL of 0.2 mM DPPH and 100 μL of the test compound, and reacted at room temperature for 20 minutes, and then used enzyme labeling Absorbance was measured at 517 nm using a microplate reader.
[0034] From image 3 As can be seen from the exper...
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