External-source radish fragment specific marker and preparation method and application thereof

Abstract

The invention relates to a cabbage type rape external-source radish fragment specific marker and a preparation method and an application thereof. The method comprises the steps that whole-genome re-sequencing data of cabbage type rape radish cytoplasm infertility restoring material CLR650 is compared with radish genome rough draft information, and the external-source radish fragment specific marker CLR9-1-CLR9-20 is developed. Through a target single plant of the cytoplasm infertility restoring material CLR650 backcross population, BC4 segregation population is obtained; a fertile plant of theBC4 population is subjected to multi-generation mixed selection by adopting a mixing method until the proportion of the obtained fertile plant is beyond 75% of mixing segregation population; finally,homozygous stability is conducted through multi-generation selfing, and a cabbage type rape radish cytoplasm infertility restoring line is obtained. The external-source radish fragment specific marker overcomes the obstacles that the heritability of the restoring gene is severely low and the homozygous stability of phenotypic trait is difficultly achieved in the prior art.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding and the field of molecular biology, and in particular relates to a specific marker for exogenous radish segments and a preparation method for specific markers for exogenous radish segments, and also relates to the application of specific markers for exogenous radish segments in the breeding of Brassica napus . Background technique [0002] Rapeseed has obvious heterosis, and is the oil crop with the most potential to cope with the shortage of edible vegetable oil and develop renewable bioenergy at home and abroad. Radish cytoplasmic male sterility (Ogu CMS) is an ideal type of sterility for the use of heterosis in Brassica napus. It is a cytoplasmic male sterility system established by Ogura, Japan in 1968, which found the source of sterility in radish. Because the sterility of the sterile system is very stable and thorough, it has very important value in the utilization of heterosis in...

AI Technical Summary

Problems solved by technology

For example, Li Xufeng reported that the restorer gene was introduced from the radish var. indigo to rapeseed, but the restorative genes of the obtained materials were all on the episomes, but could not be stably inherited; After crossing with a sterile line, the F 1 In order to genetically improve R2000, the recovered individual plants with changed markers were screened for radiation mutagenesis, but the recovered sources and molecular markers used were all from foreign research.

There is no self-developed molecular marker in our country that can be used for ogura-CMS restorer line breeding

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