Pathological tissue specimen fixative

A tissue specimen and fixative technology, applied in the field of specimen processing, can solve the problems of specimen dehydration, high cost, deformation, etc., and achieve the effect of stable performance, safe use, and less dosage

Inactive Publication Date: 2018-01-09
LUOHE MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this preservation solution contains a large amount of ethanol, and long-term use will easily cause the specimen to lose water, condense, and deform, and the raw material contains nano-silver, so the cost is high

Method used

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  • Pathological tissue specimen fixative
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A fixative solution for pathological tissue specimens, comprising the following components: 10 mL of glycerol, 32 g of sodium chloride, 0.5 g of berberine, 1.2 g of menthol, 8 g of sodium dichloroisocyanurate, 2 mL of dimethyl sulfoxide, and a film-forming assistant Agent 6g, alkylphenol polyoxyethylene ether 0.5g, pure water to 1000mL.

[0021] The film-forming aid is formed by mixing sodium alginate and chitosan at a weight ratio of 1:1.

[0022] The pathological tissue specimen fixative is prepared by the following steps:

[0023] Weigh sodium chloride and 500mL pure water and mix evenly to obtain sodium chloride solution, then add berberine and dimethyl sulfoxide mixture in sequence, stir at a high speed of 1500r / min for 15min, then add glycerol, menthol, Sodium dichloroisocyanurate, film-forming aid, alkylphenol polyoxyethylene ether, and pure water were adjusted to 1000mL, and stirred at a low speed of 500r / min for 35min to obtain the product.

Embodiment 2

[0025] A pathological tissue specimen fixative, comprising the following components: glycerol 11mL, sodium chloride 31g, berberine 0.6g, menthol 1.1g, sodium dichloroisocyanurate 9g, dimethyl sulfoxide 2.2mL, film-forming 5g of additives, 0.6g of alkylphenol polyoxyethylene ether, and dilute to 1000mL with pure water.

[0026] The film-forming aid is formed by mixing sodium alginate and chitosan at a weight ratio of 1:1.

[0027] The pathological tissue specimen fixative is prepared by the following steps:

[0028] Weigh sodium chloride and 500mL pure water and mix evenly to obtain sodium chloride solution, then add berberine and dimethyl sulfoxide mixture successively, stir at a high speed of 1600r / min for 10min, then add glycerin, menthol, Sodium dichloroisocyanurate, film-forming aids and alkylphenol polyoxyethylene ethers were adjusted to a volume of 1000mL, and stirred at a low speed of 600r / min for 25min to obtain the product.

Embodiment 3

[0030] A fixative solution for pathological tissue specimens, comprising the following components: 12 mL of glycerol, 30 g of sodium chloride, 0.7 g of berberine, 1.0 g of menthol, 10 g of sodium dichloroisocyanurate, 2.4 mL of dimethyl sulfoxide, and Auxiliary 4g, alkylphenol polyoxyethylene ether 0.7g, pure water to 1000mL.

[0031] The film-forming aid is formed by mixing sodium alginate and chitosan at a weight ratio of 1.5:1.

[0032] The pathological tissue specimen fixative is prepared by the following steps:

[0033] Weigh sodium chloride and 500mL pure water and mix evenly to obtain sodium chloride solution, then add berberine and dimethyl sulfoxide mixture in sequence, stir at a high speed of 1700r / min for 14min, then add glycerol, menthol, Sodium dichloroisocyanurate, film-forming aids, alkylphenol polyoxyethylene ether, and pure water were adjusted to 1000mL, and stirred at a low speed of 700r / min for 30min to obtain the product.

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Abstract

The invention discloses a pathological tissue specimen fixative, and belongs to the technical field of specimen processing. The pathological tissue specimen fixative is prepared from the following components: 10 to 15 mL of glycerol, 25 to 32 g of sodium chloride, 0.5 to 1.2 g of berberine, 0.5 to 1.2 g of menthol, 8 to 15 g of sodium dichloroisocyanurate, 2 to 3 mL of dimethyl sulfoxide, 2 to 6 gof film forming aid, 0.5 to 1.2 g of alkylphenol ethoxylates, and pure water to 1000 mL. The pathological tissue specimen fixative is safe and environmentally friendly, has no irritating odor, has asignificant inhibitory effect on common bacteria and has excellent fixing effect on a pathological tissue specimen.

Description

technical field [0001] The invention relates to the technical field of specimen processing, in particular to a pathological tissue specimen fixative. Background technique [0002] With the rapid development of molecular pathology technology and the continuous improvement of genetic testing technology requirements, the effect of tissue fixation is becoming more and more important. This is not only closely related to the pathological diagnosis of the disease, but also directly related to the selection of subsequent molecular detection indicators and treatment methods. . The quality of pathological slice production technology involves many links, and pathological tissue fixation is the most basic and important step. Pathological tissue fixation refers to the use of some chemical reagents to treat tissues to preserve the position, shape and structure of tissues or cells. [0003] Traditional fixatives have always been based on 40% neutral formaldehyde buffered aqueous solution...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 刘恩娜张艳王艳伟
Owner LUOHE MEDICAL COLLEGE
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