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Efficient extraction and determination method of carotenoids from Rosa spp petals

A carotene and Chinese rose technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high carotenoid degradation, large sample loss, low extraction efficiency, etc., and achieve the effect of efficient extraction

Active Publication Date: 2018-01-09
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Preliminary search results show that most of the existing studies focus on the extraction and determination of carotenoids in rose fruits, and there are disadvantages such as cumbersome operation steps, large sample loss, and low extraction efficiency.
Eugster et al. (1991) separated and identified carotenoids in rose petals using classic paper chromatography. This method not only degrades more carotenoids but also has poor separation effect

Method used

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  • Efficient extraction and determination method of carotenoids from Rosa spp petals
  • Efficient extraction and determination method of carotenoids from Rosa spp petals
  • Efficient extraction and determination method of carotenoids from Rosa spp petals

Examples

Experimental program
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Effect test

Embodiment 1

[0067] Example 1 Optimization of Carotenoid Extraction and Measurement Conditions

[0068] 1. Design 8 extraction agents to extract carotenoids from rose petals, and screen the optimal extraction agent. The 8 extraction agents are: methanol (containing 0.1% BHT), methanol-tetrahydrofuran (1:1, v / v, containing 0.1% BHT), ethanol (containing 0.1% BHT), ethanol-n-hexane ( 4:3, v / v, containing 0.1% BHT), acetone (containing 0.1% BHT), tetrahydrofuran-n-hexane (1:1, v / v, containing 0.1% BHT), n-hexane-acetone-ethanol (2: 1:1, v / v, with 0.1% BHT) and n-hexane-acetone (1:1, v / v, with 0.1% BHT).

[0069] The specific steps for preparing the sample solution to be tested are: (1) quickly grind the petals of 'Sun City' in full bloom into powder in liquid nitrogen, accurately weigh 0.2-0.3g sample and record the weight, add 4ml extractant, place in Shake and extract in a shaking incubator for 20 minutes; (2) Then add 4ml of n-hexane (containing 0.1% BHT), place in a shaking incubator an...

Embodiment 2

[0080] Example 2 Drawing of carotenoid standard curve

[0081] Accurately weigh 1mg carotenoid standard, specifically β-carotene, violaxanthin, antheraxanthin, zeaxanthin and lutein, add 10ml methanol-methyl tert-butyl ether (1:1, v / v, containing 0.1% BHT) to obtain a mother solution with a concentration of 100 μg / ml, and then dilute it by 2 times successively to prepare 6 standard solutions of concentration gradients, which are used to draw a standard curve. The standard solution was analyzed by high performance liquid chromatography to obtain the high performance liquid chromatogram of the mixed standard solution ( Figure 4 ( Figure 5 ). Chromatographic conditions were the same as in step 2 in Example 1.

Embodiment 3

[0082] Example 3 Extraction and determination of carotenoids in the petals of Chinese rose variety ‘Sun City’

[0083] 1. Prepare the solution to be tested

[0084] The petals of the modern rose variety 'Sun City' in full bloom were quickly ground into powder in liquid nitrogen, and the 0.2-0.3g sample was accurately weighed and the weight was recorded to prepare the sample solution to be tested. The method was the same as in Example 1, and the extracting agent was methanol ( with 0.1% BHT).

[0085] 2. Separation detection

[0086] (1) High performance liquid chromatography (HPLC)

[0087] The carotene dry powder was reconstituted with 1ml methanol-methyl tert-butyl ether (1:1, v / v, containing 0.1% BHT), then filtered through a 0.22μm microporous membrane, and placed in a 2ml Agilent sample bottle for use. analyzed by HPLC. Liquid chromatography conditions are the same as in Example 1. The resulting chromatogram is Image 6 shown.

[0088] (2) Liquid chromatography-ma...

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Abstract

The invention provides an efficient extraction and determination method of carotenoids from Rosa spp petals. The method comprises the following steps: a, preparing a carotenoid standard solution; b, preparing a to-be-detected carotenoid sample solution; c, performing HPLC (high performance liquid chromatography) analysis; d, performing LC-MS (liquid chromatography-mass spectrometry) analysis to obtain mass spectrum information of carotenoids in the Rosa spp petals; e, identifying the structure of the carotenoids in a Rosa spp petal sample according to retention time, UV-Vis absorption spectrogram, a mass spectrogram, a carotenoid standard substance and literature; f, performing quantitative analysis on the carotenoids in the Rosa spp petals according to a linear regression equation of thecarotenoid standard substance. The method has the advantages of low sample consumption, high extraction efficiency, good result repeatability, accurate and reliable data, and is an excellent method suitable for extraction and qualitative and quantitative analysis of the carotenoids in the Rosa spp petals.

Description

technical field [0001] The invention relates to a method for extracting carotenoids, in particular to a method for efficiently extracting and measuring carotenoids in rose petals. Background technique [0002] Chinese rose (Rosa spp.) is an ornamental plant with important economic value, and flower color is one of its most important ornamental characters. Yellow rose is a miracle in the history of rose breeding, and carotene is an important substance that gives roses yellow and orange. [0003] Carotenoids are terpenoids (mostly C40) composed of multiple isoprene skeletons connected in series. Due to the presence of a conjugated system, carotenoids appear yellow, orange or red under visible light. For plants, carotenoids have core biological functions, such as light energy transfer, light protection, and free radical scavenging in photosynthesis. Precursors of lactones. And carotenoids can give plants flowers, fruits and seeds various colors, and play an important role i...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 张启翔万会花韩瑜程堂仁王佳于超潘会堂李生蔡勇乔亚西王李亮
Owner BEIJING FORESTRY UNIVERSITY
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