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Lung cancer diagnosis kit for detecting blood autoantibody and application of lung cancer diagnosis kit

A lung cancer diagnosis and autoantibody technology, applied in the field of medical diagnosis, to achieve the effect of improving survival rate, low cost, and less pain for patients

Active Publication Date: 2018-01-09
SHANGHAI PULMONARY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no human SRSF7 (serine-rich arginine cleavage factor 7), TRNP1 (TMF regulatory nucleoprotein 1), RBM42 (RNA binding protein 42), GGA2 (ADP ribosylation factor binding protein 2), XAGE3 ( X antigen family protein 3), ZNF696 (zinc finger protein 696), FADD (Fas-associated death receptor protein), HOMER1 (homer homologous protein 1), UBQLN2 (ubiquitin 2), SNAPC2 (snRNA activating protein complex subunit Base 2) and other antigenic proteins, used to detect serum / plasma autoantibodies, and a lung cancer diagnostic kit with high sensitivity and specificity

Method used

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  • Lung cancer diagnosis kit for detecting blood autoantibody and application of lung cancer diagnosis kit
  • Lung cancer diagnosis kit for detecting blood autoantibody and application of lung cancer diagnosis kit
  • Lung cancer diagnosis kit for detecting blood autoantibody and application of lung cancer diagnosis kit

Examples

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Effect test

Embodiment 1

[0046] Example 1 Screening of Serum Autoantibodies in Patients with Lung Cancer by Protein Chip Technology

[0047] The human proteome chip (CDI Laboratories, USA) contains about 14,000 human recombinant proteins. The process of screening serum antibodies is as follows: collect serum from patients with different types of lung cancer; incubate and hybridize serum samples with protein chips to allow serum antibodies to bind to antigens immobilized on the chip; after washing, use anti-human IgG / M fluorescently labeled secondary antibodies ( Anti-human IgG-Cy3, green, anti-human IgG-Cy5, red (incubation), after washing, the signal was read with a fluorescent scanner, and the strength of the signal was positively correlated with the affinity and quantity of autoantibodies. Proceed as follows:

[0048] ①Collect serum from lung cancer patients whose lung cancer stages are I, II, III, and IV. Serum from age-matched healthy people and lung non-malignant disease were collected as control...

Embodiment 2

[0054] Embodiment 2 is set up lung cancer diagnosis kit of the present invention

[0055] (1) Expression and preparation of specific binding partners

[0056] The specific binding partners of the above-mentioned autoantibodies screened in Example 1 can be synthesized by genetic engineering methods or artificial synthesis methods known to those skilled in the art. If genetic engineering is used for synthesis, prokaryotic expression system or eukaryotic expression system can be selected according to needs. The prokaryotic expression system is mainly represented by E. coli expression system, and the eukaryotic expression system is yeast expression system, insect cell expression system, mammalian Animal expression systems are represented. If the method of artificial synthesis is adopted, the synthesis of specific antigen fragments can be carried out directly through the classical solid-phase peptide synthesis method. No matter which method is used, the purpose is to obtain the i...

Embodiment 3

[0144] Embodiment 3 Determining the specificity and sensitivity of the lung cancer diagnostic kit of the present invention for diagnosing lung cancer

[0145] Collect 45 cases of serum from patients with stage I and II lung cancer, 30 cases of serum from patients with lung non-malignant diseases, and 15 cases of age-matched healthy human serum, and use the enzyme-linked immunosorbent assay (EIA) kit of the present invention to detect serum autoantibodies. Specific steps are as follows:

[0146] ① Take the microwell plate coated with the specific binding partner, add 100 μL of sample serum, and incubate at room temperature for 90 minutes;

[0147] ② Wash with PBS three times, 5 minutes each time;

[0148] ③ Add 100 μL Anti-IgG-HRP secondary antibody working solution and incubate at room temperature for 1 hour;

[0149] ④ Wash with PBS three times, 5 minutes each time;

[0150]⑤ Add 200 μL of TMB color developing solution, and develop color at 25°C for 10 minutes. TMB Chromo...

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Abstract

The invention relates to a lung cancer diagnosis kit for detecting a blood autoantibody and application of the lung cancer diagnosis kit. The invention provides application of any one or more of proteins containing SRSF7, TRNP1, RBM42, GGA2, XAGE3, ZNF696, FADD, HOMER1, UBQLN2 and SNAPC2 or fragments thereof in preparing a lung cancer diagnosis reagent or kit. The invention also provides the lungcancer diagnosis kit; the lung cancer diagnosis kit comprises a solid matrix, a specifically-bound gametophyte of protein autoantibodies, a second specifically-bound gametophyte and a detectable group. The kit disclosed by the invention has the advantages of simply and quickly diagnosing lung cancer, including early diagnosis of the lung cancer, risk prediction of suspected lung cancer, benign andmalignant grouping, recurrence monitoring, medication concomitant diagnosis and the like, and has excellent sensitivity and specificity and high diagnostic efficiency.

Description

technical field [0001] The invention relates to the technical field of medical diagnosis, in particular to a lung cancer diagnostic kit for detecting specific autoantibodies in blood and its application. Background technique [0002] The World Health Organization's Global Burden of Disease Study shows that cancer is the second highest mortality disease in the world and in China after cardiovascular disease. Early diagnosis of cancer is of great significance to the selection and curative effect of cancer treatment. However, for many types of cancer, there is still a lack of good early diagnostic indicators and screening techniques, resulting in an advanced diagnosis. Taking lung cancer as an example, although the popularization of medical imaging technology has improved the ability of early detection of lung cancer, most lung cancer patients are diagnosed with advanced diseases. Even if small pulmonary nodules are found on medical imaging, there are quite a few patients who...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
Inventor 粟波丁茜徐雯唐亮
Owner SHANGHAI PULMONARY HOSPITAL
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