Exosome targeted slow-release microsphere biological stent and preparation method and use thereof

A technology of slow-release microspheres and bio-scaffolds, applied in the field of medical biomaterials, can solve problems such as difficult formation of target sites, inability to concentrate MSC-Exo, targeted drug delivery, etc., to achieve effective activity retention and excellent sustained-release control effects , good targeted effect

Active Publication Date: 2018-01-16
李征宇 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still many problems in the use of MSC-Exo in the treatment of diseases. The most important point is that the existing methods of drug delivery, such as intravenous injection, intranasal administration, and intracerebroventricular administration, are easy to spread, resul

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  • Exosome targeted slow-release microsphere biological stent and preparation method and use thereof
  • Exosome targeted slow-release microsphere biological stent and preparation method and use thereof
  • Exosome targeted slow-release microsphere biological stent and preparation method and use thereof

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Embodiment Construction

[0037] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0038] 1. Main reagents and instruments

[0039] Pepsin, Chloroform, Glacial Acetic Acid, NaHCO 3 , NaCl, high-speed centrifuge, freeze dryer, deep-low temperature refrigerator, meat grinder, dialysis membrane, liquid nitrogen planetary ball mill, tissue mash homogenizer.

[0040] 2. Method

[0041] 2.1 Collagen preparation

[0042] (1) Pretreatment: take fresh bovine (pig, sheep or other animal) Achilles tendon, bovine (pig, sheep or other animal) skin, bovine (pig, sheep or other animal) articular cartilage and other biological materials (for example, take skin), wash and remove subcutaneous fat and dermis, remove fascia, remove hair, and chop finely.

[0043] (2) Degreasing: The shredded biological material is washed with double distilled water, minced in a meat grinder, soaked in a mixed solvent of chloroform-methanol (for example, vol...

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Abstract

The invention provides an exosome targeted slow-release microsphere biological stent and a preparation method and a use thereof; the biological stent includes collagen, degradable microspheres dispersed in the collagen, and an exosome combined on the microspheres. The preparation method comprises the steps: separating collagen from animal tissues; separating an exosome from a culture substance ofbone marrow or umbilical cord mesenchymal stem cells; preparing microspheres combined with the exosome by an emulsification-crosslinking method; and mixing the microspheres with the collagen, and freeze-drying. Chitosan and other high molecular materials are combined with the exosome by a microsphere structure, the microsphere structure is dispersed to the collagen, a biological stent material having a function of directional release of the exosome is formed, the exosome targeted repairing and treatment effects can be significantly improved, and the microsphere structure makes the release of the exosome have slow-release controllable characteristics, accords with development trend of accurate drug diversity, has diverse dosage forms, and has broad clinical application value.

Description

technical field [0001] The invention belongs to the field of medical biomaterials, in particular to an exosome-targeted slow-release collagen bioscaffold. Background technique [0002] Domestic and foreign studies have confirmed that bone marrow or umbilical cord mesenchymal stem cell exosomes (MSC-derived exosomes, MSC-Exo) have obvious immune regulation and neuroprotective functions. Compared with simple cell therapy, MSC-Exo is easier to penetrate the skin, Mucous membrane, nerve sheath, fascia, blood-brain barrier and post-traumatic damaged tissue can avoid the risk of cell tumorigenesis, and it is convenient for freeze-thaw storage. It is comprehensively suggested that MSC-Exo can become a more ideal clinical bioremediation agent. However, there are still many problems in the use of MSC-Exo in the treatment of diseases. The most important point is that the existing methods of drug delivery, such as intravenous injection, intranasal administration, and intracerebroventri...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K47/42A61K9/16A61K47/36A61P25/00A61P17/00A61P19/04A61P25/08A61P25/28A61P9/10
Inventor 李征宇龙乾发
Owner 李征宇
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