Exosome targeted slow-release microsphere biological stent and preparation method and use thereof

Abstract

The invention provides an exosome targeted slow-release microsphere biological stent and a preparation method and a use thereof; the biological stent includes collagen, degradable microspheres dispersed in the collagen, and an exosome combined on the microspheres. The preparation method comprises the steps: separating collagen from animal tissues; separating an exosome from a culture substance ofbone marrow or umbilical cord mesenchymal stem cells; preparing microspheres combined with the exosome by an emulsification-crosslinking method; and mixing the microspheres with the collagen, and freeze-drying. Chitosan and other high molecular materials are combined with the exosome by a microsphere structure, the microsphere structure is dispersed to the collagen, a biological stent material having a function of directional release of the exosome is formed, the exosome targeted repairing and treatment effects can be significantly improved, and the microsphere structure makes the release of the exosome have slow-release controllable characteristics, accords with development trend of accurate drug diversity, has diverse dosage forms, and has broad clinical application value.

Description

technical field [0001] The invention belongs to the field of medical biomaterials, in particular to an exosome-targeted slow-release collagen bioscaffold. Background technique [0002] Domestic and foreign studies have confirmed that bone marrow or umbilical cord mesenchymal stem cell exosomes (MSC-derived exosomes, MSC-Exo) have obvious immune regulation and neuroprotective functions. Compared with simple cell therapy, MSC-Exo is easier to penetrate the skin, Mucous membrane, nerve sheath, fascia, blood-brain barrier and post-traumatic damaged tissue can avoid the risk of cell tumorigenesis, and it is convenient for freeze-thaw storage. It is comprehensively suggested that MSC-Exo can become a more ideal clinical bioremediation agent. However, there are still many problems in the use of MSC-Exo in the treatment of diseases. The most important point is that the existing methods of drug delivery, such as intravenous injection, intranasal administration, and intracerebroventri...

AI Technical Summary

Problems solved by technology

However, there are still many problems in the use of MSC-Exo in the treatment of diseases. The most important point is that the existing methods of drug delivery, such as intravenous injection, intranasal administration, and intracerebroventricular administration, are easy to spread, resulting in the inability of MSC-Exo to concentrate on the site. Therefore, it is urgent to construct a drug-loading system that can realize the targeted / directional sustained-release therapeutic effect of MSC-Exo, so as to fully utilize the potential wide range of therapeutic uses of MSC-Exo

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