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Combined growth regulator containing pantoea polysaccharide, and application of combined growth regulator

A growth regulator and combined technology, applied in the field of agriculture, can solve the problems of crop growth, human health hazards, easy loss, etc., and achieve the effect of increasing the action time, improving the utilization rate, and reducing the dosage

Pending Publication Date: 2020-11-10
NANJING SHINEKING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently some problems with plant growth regulators: (1) easy to lose and low synergistic effect; (2) severe phytotoxicity, which may cause excessive growth of crops, or inhibit growth, or even die; (3) some Regulators will have a certain impact on the quality of agricultural products and cause harm to human health
Synthetic brassinolides have high activity and can be absorbed by leaves, stems, and roots of plants, and then transmitted to the site of action. However, there is no unified view on the mechanism of action, mainly because it can increase the activity of RNA polymerase , increase the content of RNA and DNA; increase the potential difference of the cell membrane and the activity of ATPase; and strengthen the effect of auxin, etc.

Method used

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  • Combined growth regulator containing pantoea polysaccharide, and application of combined growth regulator
  • Combined growth regulator containing pantoea polysaccharide, and application of combined growth regulator
  • Combined growth regulator containing pantoea polysaccharide, and application of combined growth regulator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: the preparation of pantoea polysaccharide

[0048] The strain used is Pantoea alhagi XK-11, and the preservation number is CGMCC NO.15526. It has been preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures. The preservation time is March 29, 2018. The preservation address is Beichenxi, Chaoyang District, Beijing Yard No. 3, No. 1 Road, is a plant endophyte Pantoea camelium. The specific preparation method of pantoea polysaccharide is as follows:

[0049] (1) Pick P.alhagi XK-11 into fresh LB liquid medium, culture at 37°C and 200rpm for 12 hours to obtain seed liquid;

[0050](2) The seed solution is transferred to the fermentation medium of pantoea polysaccharide (35g / L sucrose; 5.5g / L peptone; 1g / L MgSO 4 ;2g / L K 2 HPO 4 ), the fermentation temperature is 30°C, the pH is 6.5, the aeration rate is 0.7vvm, the stirring speed is 700rpm, and the fermentation is carried out for 24 hours to obtain the ferme...

Embodiment 2

[0055] Embodiment 2: Suspension preparation of the combined growth regulator containing pantoea polysaccharide

[0056] Prepare combined growth regulator as follows:

[0057] (1) Weighing the active ingredients of 0.02% pantoea polysaccharide, 0.08% thiadizuron, and 0.1% brassinolide according to mass percentage;

[0058] (2) Take by weight the auxiliary materials of 6% lignosulfonate, 1.5% Tween-60, 0.6% benzoic acid, 0.6% organic silicon, 0.4% urea, and 2% bentonite;

[0059] (3) Gradually add the above-mentioned ingredients to deionized water from low to high according to the percentage (make up to 100% of the total weight with deionized water), and keep stirring and mixing the ingredients. After all the additions are complete, disperse at high speed for 30 minutes ;

[0060] (4) The suspension of the combined growth regulator containing pantoea polysaccharide was prepared by sanding with a sand mill.

Embodiment 3

[0061] Example 3: Preparation of a dispersed oil suspension of a combined growth regulator containing pantoea polysaccharide.

[0062] Prepare combined growth regulator as follows:

[0063] (1) Weighing the active ingredients of 2% pantoea, 1% thiadizuron, and 0.5% brassinolide according to mass percentage;

[0064] (2) Take by weight the auxiliary materials of 6% lignosulfonate, 1.5% Tween-60, 0.6% benzoic acid, 0.6% organosilicon, and 0.4% urea;

[0065] (3) Gradually add the above-mentioned ingredients to the soybean oil according to the percentage from low to high (make up to 100% of the total weight with soybean oil), and constantly stir and mix the ingredients. After all the addition is completed, disperse at high speed for 30 minutes;

[0066] (4) After sanding with a sand mill, a dispersed oil suspension concentrate of a combined growth regulator containing pantoea polysaccharide was prepared.

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Abstract

The invention discloses a combined growth regulator containing pantoea polysaccharide, wherein the combined growth regulator is characterized by consisting of the following components in percentage bymass: 0.02-5% of pantoea polysaccharide, 0.005-1% of thidiazuron, 0.003-0.5% of brassinolide, and the balance of an auxiliary material, wherein the pantoea polysaccharide is a product prepared by fermenting pantoea alhagi. According to the invention, thidiazuron and brassinolide are compounded to achieve a remarkable growth promoting effect, and pantoea polysaccharide serving as a synergist can fully chelate effective components, assists the effective components to be attached to the surfaces of crops and is not prone to loss along with water, so that the pesticide effect is enhanced.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a combined growth regulator containing pantoea polysaccharide. Background technique [0002] With the increase of population and the improvement of people's living standards, the rigid demand for agricultural products is increasing year by year. Under the current situation of decreasing cultivated land area, it is necessary to increase the yield per unit area of ​​crops and the quality of agricultural products. In recent years, plant growth regulators have been widely used in my country, and the demand is increasing year by year. [0003] Plant growth regulators can effectively regulate the growth and development of plants, including the growth and division of plant cells, and a series of plant life processes such as rooting, germination, flowering, fruiting, maturation and shedding. Used in agricultural production, it can effectively regulate the growth process of crops, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/36A01N43/22A01N43/16A01P21/00C05G3/00
CPCA01N47/36A01N43/22A01N43/16C05G3/00
Inventor 雷鹏梁金丰殷文锋
Owner NANJING SHINEKING BIOTECH CO LTD
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