Rapid identification method for carbapenem drug susceptibility, based on Raman spectra technology

Abstract

The invention relates to a rapid identification method for carbapenem drug susceptibility, based on a Raman spectra technology. According to the method, a portable Raman detector is adopted, pathogenic bacteria to be detected is irradiated through laser, different types of nanostructures are taken as detection substrates, and scattered spectrum, the frequency of which is different from the frequency of incident light, is analyzed, so that information, such as molecular vibration, of a sample to be detected is obtained, corresponding molecular structure analysis is performed, obtained spectroscopic data of carbapenem drug sensitive bacteria and drug-resistance bacteria is analyzed through a chemometrics method, so that drug-resistance bacteria and sensitive bacteria are distinguished, and the method becomes an ultrasensitive pathogenic bacteria rapid detection tool. Compared with the clinically traditional drug sensitivity pathogenic microorganism detection method, the detection technology has the advantages that the quantity of samples is less, the detection time is short, the sensitivity is high, the detection time of clinical samples is shortened, and particularly as for detection samples with complex chemical and biochemical components, the detection efficiency is improved on the basis that the detection quality is guaranteed.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a rapid identification method for carbapenem drug sensitivity based on Raman spectroscopy. Background technique [0002] In recent years, the spread of infectious diseases caused by pathogenic bacteria has shown an obvious upward trend in my country; therefore, in the fields of medicine, veterinary medicine, water and food safety, rapid clinical detection and identification of pathogenic bacteria is becoming increasingly important. Carbapenem antibacterial drugs are a type of atypical β-lactam antibacterial drugs with the broadest antibacterial spectrum and strongest antibacterial activity at present, and are most effective for treating multidrug-resistant Gram-negative bacteria. However, with its widespread clinical use, carbapenem-resistant strains of some common clinical Gram-negative bacteria have emerged, which has brought great challenges to clinical anti-infective treat...

AI Technical Summary

Problems solved by technology

[0003] The identification of traditional drug-sensitive pathogens requires cumbersome steps and long-term cultivation, which is time-consuming and laborious; traditional pathogen detection methods can no longer meet the needs of early and rapid detection of drug-sensitive pathogens; other detection methods such as PCR and immunological detection and other technologies have the advantages of fast detection speed, but they still have certain limitations in terms of detection specificity, sensitivity, and detection speed.

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