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Composition for three-dimensional printing, method for preparing same, and method for manufacturing three-dimensional structure using same

A technology of three-dimensional printing and composition, applied in the direction of prosthesis, tissue regeneration, medical science, etc., can solve the problems of difficult preparation of organs, safety problems, low mechanical strength of three-dimensional structures, etc., and achieve high mechanical strength and uniform mechanical strength. and the effect

Inactive Publication Date: 2018-01-16
POSTECH ACAD IND FOUND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical strength of the three-dimensional structure obtained by gelation through thermal processing is relatively low, making it difficult to prepare organs requiring satisfactory mechanical strength
In addition, post-printing cross-linking requires the use of toxic cross-linking agents such as glutaraldehyde, leading to safety issues, and insufficient cross-linking inside the 3D structure, resulting in unevenly cross-linked 3D structures. body

Method used

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  • Composition for three-dimensional printing, method for preparing same, and method for manufacturing three-dimensional structure using same
  • Composition for three-dimensional printing, method for preparing same, and method for manufacturing three-dimensional structure using same
  • Composition for three-dimensional printing, method for preparing same, and method for manufacturing three-dimensional structure using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Preparation of composition for three-dimensional printing

[0039] Liquid nitrogen was added to the freeze-dried hdECM and crushed with a mortar and pestle. After adding hdECM powder (330 mg) to 0.5 M acetic acid aqueous solution (10 ml), pepsin (33 mg) (P7125, Sigma-Aldrich) was added, and it stirred at normal temperature for 48 hours. While maintaining the temperature of the obtained solution below 10°C, riboflavin (2 mg) was added, and a 10M NaOH solution cooled to below 10°C was added dropwise to adjust the pH to about 7. The obtained solution in the form of a pre-gel (pre-gel) was refrigerated and stored at about 4°C.

Embodiment 2

[0040] Embodiment 2: Preparation of three-dimensional structure

[0041]A three-dimensional structure was fabricated using the composition for three-dimensional printing obtained in Example 1 according to the method disclosed in Falguni Pati, et al., Nat Commun. 5, 3935 (2014). Specifically, the polycaprolactone (PCL) framework was loaded into the syringe of the multi-head tissue and organ printing system (Jin-Hyung Shim et al., J. Micromech. Microeng. 22 085014 (2012)) ), and heated to about 80°C, causing the polymer to melt. The three-dimensional printing composition in pregel form obtained in Example 1 was charged into another syringe (second syringe), and the temperature was maintained at about 10° C. or lower. Apply an air pressure of about 600 kPa to the first syringe, thereby fabricating a thin PCL frame with a line width of about 100 μm or less, a gap of about 300 μm, and a thickness of 120 μm, and spray the contents of the second syringe on the PCL frame, and then in...

Embodiment 3

[0043] Embodiment 3: Preparation of three-dimensional structure

[0044] A three-dimensional structure was prepared by the same method as in Example 2 except that the PCL framework was not used. That is, the composition for three-dimensional printing in the pregel form obtained in Example 1 was loaded into a syringe of a multi-head tissue and organ printing system (Jin-HyungShim et al., J. Mieromech. Microeng. 22 085014 (2012)), and The temperature was maintained below about 10°C. The contents were ejected by applying an air pressure of about 600 kPa to the syringe, and then irradiated with UVA at about 360 nm for 3 minutes to perform cross-linking. Thereafter, the content of the syringe is ejected and a layer-by-layer process through the cross-linking is performed to form a three-dimensional structure shape. The obtained shape of the three-dimensional structure was placed in an incubator (humid incubator) at about 37° C. for 30 minutes to perform thermal gelation, whereby a...

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Abstract

The present invention provides a composition for three-dimensional printing, containing: a decellularized extracellular matrix; and riboflavin as a cross-linking agent. A three-dimensional structure having high mechanical strength can be manufactured by performing a layer-by-layer process through printing using the composition for three-dimensional printing according to the present invention and cross-linking under UVA to prepare a three-dimensional structure shape and then subjecting the thus obtained three-dimensional structure shape to thermal gelation. Furthermore, the present invention provides a method for preparing the composition for three-dimensional printing, and a method for manufacturing a three-dimensional structure using the composition for three-dimensional printing.

Description

technical field [0001] The present invention relates to a composition for three-dimensional printing. In addition, the present invention relates to a method for producing the composition for 3D printing and a method for producing a three-dimensional structure using the composition for 3D printing. Background technique [0002] 3D printing refers to transforming arbitrary shape information obtained from medical data of complex-shaped tissues or organs into G-codes and constructing complex skeleton structure. This three-dimensional printing is also called "three-dimensional bioprinting (3D bioprinting)". For example, the "multi-head tissue and organ printing system" is one of the representative three-dimensional printing technologies, which consists of two pneumatic injectors that inject materials through air pressure and two injectors that use step motors to precisely inject materials in nanoliter units. Composed of plunger syringes, and can use multiple materials at the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50B33Y10/00B33Y70/00
CPCA61L2430/40B33Y70/00A61L27/3633A61L27/3687B33Y10/00A61L27/18A61L27/227A61L27/26
Inventor 曹东佑张珍娥
Owner POSTECH ACAD IND FOUND
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